頭頸部癌細胞におけるレスベラトロールのREG III発現誘導効果と癌の進展抑制・治療向上効果

Identification of reliable markers of chemo- and radiosensitivity and the key molecules that enhance the susceptibility of head and neck squamous cell carcinoma (HNSCC) to anticancer treatments is highly desirable. Previously, we have reported that regenerating gene (REG) Ⅲ expression was such a marker associated with an improved survival rate for HNSCC patients. In the present study, we investigated the stimulators for induction of REG Ⅲ expression using REG Ⅲ promoter assay in HNSCC cells transfected with REG Ⅲ promoter vector. We tested inflammatory cytokines, growth factors, polyphenols, PPARγ activator of thiazolidinediones, and histone deacetylase inhibitors, and found that 3,4',5-trihydroxy-trans-stilbene (resveratrol) significantly increased the REG Ⅲ promoter activity and the mRNA levels of REG Ⅲ in HNSCC cells. Moreover, we demonstrated the effect of resveratrol on cancer cell progression, such as cell proliferation, chemo‑ and radiosensitivity and cancer invasion of HNSCC cells. Resveratrol significantly inhibited cell growth, enhanced chemo‑ and radiosensitivity, and blocked cancer invasion of HNSCC cells. These data suggested that resveratrol could inhibit cancer progression through the REG Ⅲ expression pathway in HNSCC cells.博士（医学）・甲第682号・平成30年3月15日Copyright © Spandidos Publications 2017. All rights reserved.The Spandidos Publications link is available at " https://doi.org/10.3892/ijo.2016.3664

Effect of Asian dust on pulmonary function in adult asthma patients in western Japan: A panel study

AbstractBackgroundAsian dust (AD) has become a major health concern. The concentration of AD is typically expressed in particulate matter less than 10 μm (PM10) and 2.5 μm (PM2.5). However, PM10 and PM2.5 consist of various substances besides AD. Light detection and ranging (LIDAR) systems can selectively measure the quantity of AD particles to distinguish non-spherical airborne particles from spherical airborne particles. The objective of this study was to investigate the relationship between pulmonary function in adult asthma patients and AD using LIDAR data.MethodsSubjects were 231 adult asthma patients who had their morning peak expiratory flow (PEF) measured from March to May 2012. A linear mixed model was used to estimate the association of PEF with sand dust particles detected by LIDAR.ResultsIncreases in the interquartile range of AD particles (0.018 km−1) led to changes in PEF of −0.42 L/min (95% confidence interval [CI], −0.85 to 0.01). An increase of 11.8 μg/m3 in suspended particulate matter and 6.9 μg/m3 in PM2.5 led to decreases of −0.17 L/min (−0.53 to 0.21) and 0.03 L/min (−0.35 to 0.42), respectively. A heavy AD day was defined as a day with a level of AD particles >0.032 km−1, which was the average plus one standard deviation during the study period, and six heavy AD days were identified. Change in PEF after a heavy AD day was −0.97 L/min (−1.90 to −0.04).ConclusionsHeavy exposure to AD particles was significantly associated with decreased pulmonary function in adult asthma patients

Physical Image Quality of Rapid Processing Films

The imaging properties of Konica "new" SR series films and "old" MG-SR series films for rapid processing were measured in combination with green-emitting intensifying screens. The measured image quality prarameters include: characteristic curve, modulation transfer function (MTF) and Wiener spectrum. The relative speeds of the new SR series systems were slower than those of the old MG-SR series systems from 10 to 25%. The MTF's of the new systems were generally comparable to those of the old systems, except that SR-G system was improved with an increase of approximately 10%. In the Wiener spectrum measurement, however, the new systems showed from 10 to 35% decrease in low-frequency noise

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target