The effects of detergent, sodium tripoly-phosphate and ethoxyled oleyl-cetyl alcohol on metabolic parameters of the fungus trichothecium roseum link

The degradation of detergents that are dispersed in water and soil partially depends on the metabolic activities of fungi. Among the fungi that have this ability, Deuteromycetes are particularly noted for their biochemical characteristics. Taking this into account, it was of interest to analyze the influence of detergent and its main compounds, ethoxyled oleylcetyl alcohol (AOC) and sodium tripoly-phosphate (TTP), on the metabolism of the fungus Trichothecium roseum. Our results revealed that both detergent and AOC had an inhibitory effect on the bioproduction of free organic acids, while TTP stimulated their production. Also, detergent inhibited the bioproduction of basic amino acids, with the exception of alanine. In addition, detergent applied at 1% concentration inhibited the bioproduction of proteins and the total biomass of the fungus, while AOC and TTP inhibited the production of proteins, but stimulatedl the production of Trichothecium

The influence of detergents, sodium tripoly-phosphates and ethoxyled oleyl-cetyl alcohol on metabolism of the fungi Penicillium Verrucosum peyronel

On the species of the fungi Penicillium verrucosum grown on liquid nutritious base, according to Czapek and on a variation of the same nutritious base with detergent MERIX ('Merima, Kruševac) and individual components of the same detergent: sodium tripolyphosphate and ethoxyled oleyl-cetyl alcohol in a concentration of 0.1 %, the following analyses were performed: pH, redox potential, proteolytic activity, the quantity of free and total organic acids, amino acids, proteins and total biomass. We can notice from the obtained results that the detergent, sodium tripoly-phosphate and ethoxyled oleyl-cetyl alcohol in a concentration of 0.1% had an inhibitory effect on protein bioproduction and total biomass on the 8th day from the day of inoculation, while the production of free and total organic acids was partially or significantly stimulated by the presence of the detergent, sodium tripoly-phosphate and ethoxyled oleyl-cetyl alcohol in a concentration of 0.1 %. With aging of the fungus culture Penicillium verrucosum, the exception was free organic acids production which was, with the age of the culture, partially inhibited by the detergent. Proteolytic activity of the fungi Penicillium verrucosum had a slightly variable value during the experimental period with added nutritions media to the detergent, sodium tripoly-phosphate and ethoxyled oleyl-cetyl alcohol in a concentration of 0.1% with a slight aberration in relation to the control in some phases of the fungal growth

Free light chains of immunoglobulin as a prognostic factor for some plasmaproliferative diseases

Quantitation of monoclonal immunoglobulins and their fragments is used for monitoring the plasmaproliferative disease course and the effect of therapy. The aim of free light chains examination was to evaluate the significance of the FLC ratio as a prognostic factor for remission, progression and survival in different disease groups. The concentrations of immunoglobulins and free light chains were measured by an immunonephelometric method on a SIEMENS DADE BN II analyser with reagents (Freelite, The Binding Site, UK). In this examination 151 patients from 3 different disease groups: 1. Light chain disease or Bence Jones myeloma (37), 2. Biclonal gammopathy with FLC (23) and 3. Monoclonal gammopathy of undetermined significance (91), were investigated during a period of 7 years. The reference interval for FLC ratio is 0.26-1.65. According to the International Staging System for multiple myeloma, a serum FLC ratio of 32 was taken as abnormal. The patients with light chain disease and biclonal gammopathy with FLC with an abnormal FLC ratio and a combination of adverse risk factors (76.7%) had median survival times of 22-30 months, versus patients with a normal or slightly varied FLC ratio without adverse risk factors (23.3%) with median survival times of 39-51 months. About 38% of patients who had shown lowered free light chains values by more than 50% under therapy, achieved disease remission in the light chain disease and biclonal gammopathy with FLC groups. In the group of patients with monoclonal gammopathy of undetermined significance, 66.0% had a normal or slightly modified FLC ratio which corresponds to low and low-intermediate risk of disease progression, as opposed to 34.0% with an abnormal FLC ratio (4) which corresponds to high and high-intermediate risk. An abnormal FLC ratio in the examined groups could be an independent risk factor for progression and poorer disease prognosis

Analysis of the amino acids of some types of fungi cultivated in the presence of detergent

The paper presents the results of the influence of anionic-type detergent containing sodium tripolyphosphate and ethoxylated oleylcetyl alcohol on the type and quantity of present amino acids in the fermentation broths of the fungi Aspergillus niger, Alternaria tenuis and Fusarium oxysporum cultivated on a medium with the addition of 1% detergent as a function of their application in the process of detergent biodegradation. In the case of A.niger the stimulation of the biosynthesis of 15 various amino acids was determined. In the case of A.tenuis the production of 14 amino acids was inhibited as compared to the total number of 15 amino acids identified when the fungi were cultivated in the absence of detergent, whereby the explicit inhibition of the synthesis of methionine, isoleucine and leucine was registered. Detergent (1% concentration) exhibits a stimulating effect on the bioproduction of amino acids in the case of the fungus F.oxysporum when the presence of 14 amino acids was identified