46 research outputs found

    Swelling and Ccnsolidaticn Characieristics of the Permian Clay

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    Civil Engineerin

    Coliform Bacteria and Hatching Egg Disinfectants

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    (This study aimed to monitoring the microbial status of broiler breeder hatching eggs through isolation of coliform bacteria, especially Salmonella and E coli from eggs and hatched chicks and identification of isolated microorganism by PCR. Besides, the reduction of the contamination rate by using different egg disinfectants. A total of 600 broiler breeder Saso fertile eggs was purchased from a commercial broiler breeder flock aged 27 wk, which reared on a deep litter system at a private farm in Gharbiya Governorate, Egypt. The eggs were allocated randomly into four equal treatment groups (n?150) according to the disinfectant used. The eggs in the first group were kept as a control without treatment. While, the eggs in the second group were disinfected by 1.4% H2O2. The eggs in the third group were disinfected by 0.5

    Impact of Boiler Breeders Hatching Eggs Disinfection Time on Some Hatchability Parameters

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    (Control of microorganisms on hatching eggshell surface requires an effective disinfectant to kill the pathogens without injury to the live chick embryo. The present experiment was carried out to study the effect of hatching egg sanitization time by different disinfectants on some hatchability parameters

    The Microbiome Stress Project: Toward a Global Meta-Analysis of Environmental Stressors and Their Effects on Microbial Communities

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    Microbial community structure is highly sensitive to natural (e.g., drought, temperature, fire) and anthropogenic (e.g., heavy metal exposure, land-use change) stressors. However, despite an immense amount of data generated, systematic, cross-environment analyses of microbiome responses to multiple disturbances are lacking. Here, we present the Microbiome Stress Project, an open-access database of environmental and host-associated 16S rRNA amplicon sequencing studies collected to facilitate cross-study analyses of microbiome responses to stressors. This database will comprise published and unpublished datasets re-processed from the raw sequences into exact sequence variants using our standardized computational pipeline. Our database will provide insight into general response patterns of microbiome diversity, structure, and stability to environmental stressors. It will also enable the identification of cross-study associations between single or multiple stressors and specific microbial clades. Here, we present a proof-of-concept meta-analysis of 606 microbiomes (from nine studies) to assess microbial community responses to: (1) one stressor in one environment: soil warming across a variety of soil types, (2) a range of stressors in one environment: soil microbiome responses to a comprehensive set of stressors (incl. temperature, diesel, antibiotics, land use change, drought, and heavy metals), (3) one stressor across a range of environments: copper exposure effects on soil, sediment, activated-sludge reactors, and gut environments, and (4) the general trends of microbiome stressor responses. Overall, we found that stressor exposure significantly decreases microbiome alpha diversity and increases beta diversity (community dispersion) across a range of environments and stressor types. We observed a hump-shaped relationship between microbial community resistance to stressors (i.e., the average pairwise similarity score between the control and stressed communities) and alpha diversity. We used Phylofactor to identify microbial clades and individual taxa as potential bioindicators of copper contamination across different environments. Using standardized computational and statistical methods, the Microbiome Stress Project will leverage thousands of existing datasets to build a general framework for how microbial communities respond to environmental stress
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