Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

The version of record of this article, first published in Journal of Material Cycles and Waste Management, is available online at Publisher’s website: https://doi.org/10.1007/s10163-024-01974-y.The use of waste activated sludge (WAS) as a biocatalyst to produce polyhydroxyalkanoates (PHA) from waste streams may help promote the beneficial use of WAS for low-carbon, sustainable wastewater treatment. However, it remains unclear which types of substrates can be used for efficient PHA production, and how the PHA production can be maximized. This study aimed to assess the substrate versatility of mixed microbial cultures (MMCs) constructed from WAS by enriching PHA-accumulating bacteria using an aerobic dynamic discharge (ADD) process fed with acetate. Twelve different substrates, including organic acids, saccharides, and alcohols, were selected as the test substrates. In single-batch assays, the highest PHA production (583–680 mg/L) was achieved using butyrate, acetate, and pyruvate. In fed-batch assays, > 30 wt% PHA content was achieved using acetate, butyrate, propionate, lactate, and ethanol, with the highest content (60.3 wt%) using acetate. These results indicate that acetate-fed MMC by the ADD process could efficiently produce PHA from volatile fatty acids, lactate, pyruvate, and ethanol. Polyhydroxybutyrate was preferentially produced from acetate, butyrate, pyruvate, lactate, and ethanol, whereas polyhydroxyvalerate was notably produced from propionate. The results suggest that PHA can be efficiently produced from a wide range of substrates using MMCs enriched on a single substrate

DNA Microarray Analysis of Temporal and Spatial Variation of Bacterial Communities in Japanese Rivers

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2008.4.1～2009.3.31), pp.24～35, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2009.5 に掲

Multiple Detection of Occurrence of Bacterial Pathogens in Two Rivers in the Kinki District of Japan with a DNA Microarray

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2008.4.1～2009.3.31), pp.105～117, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2009.5 に掲

Characterization of Novel 4-n-Butylphenol- Degrading Pseudomonas Veronii Strains Isolated from Rhizosphere of Giant Duckweed, Spirodela polyrrhiza

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2009.4.1～2010.3.31), pp.32-41, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2010.5 に掲

Transfer of Plasmid pJP4 from Escherichia coli to Activated Sludge Bacteria by filter Mating

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2009.4.1～2010.3.31), pp.101-107, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2010.5 に掲

IDENTIFICATION OF SOME PREDOMINANT BACTERIA ISOLATED FROM JETA1 FUEL IN VIETNAM BY SEQUENCE ANALYSIS OF 16S RRNA GENE

Joint Research on Environmental Science and Technology for the Eart

EVALUATION OF ACCELERATED STABILIZATION OF LANDFILL BY MONITORING FUNCTIONAL MICROBES

Joint Research on Environmental Science and Technology for the Eart

カイスイチュウ ニオケル ビスフェノールA オヨビ ビスフェノールF ノ セイ ブンカイ ポテンシャル ノ ヒョウカ

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2010.4.1～2011.3.31), pp.95-102, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2011.5 に掲

Investigation by Imaging Mass Spectrometry of Biomarker Candidates for Aging in the Hair Cortex

BACKGROUND: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. METHODS: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. RESULTS: Among the 31 molecules detected specifically in hair sections, 2--one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)--exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. CONCLUSION: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution