Minigene splicing assay of patients synonymous substitutions.

<p>Effect of synonymous <i>BRCA1</i> substitutions on splicing products full-length, D(11q) and D(11). RT-PCR products from transfection experiments using mutated minigenes are shown.</p

Minigene splicing assay of BRCA1 exon 11.

<p><b>A</b>. Sequence of the critical region in exon 11 showing the deletion 1,2,3,4 (highlighted). The donor site (5′ss) generating D11q isoform is boxed. Arrows indicate nucleotide positions of the variations reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037255#pone-0037255-g002" target="_blank">Figure 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037255#pone-0037255-g003" target="_blank">3</a> to affect splicing. <b>B</b>. Transient transfection results for the hybrid minigenes carrying deletions.</p

Minigene splicing assay of BRCA1 exon 11.

<p><b>A</b>. The pB1 wild type (WT) version of the minigene is shown. PCMV = promoter of the pCDNA3 vector. ATG = start codon. TAG = stop codon. +3C = insertion of cytosine as the third nucleotide in exon 8. pA = poly A signal. 1 = exon 1 of the alfa globin gene. BRCA1 exons from 8 to 12 are numbered. The black solid line represents introns. Dotted lines show alternative splicing of exon 11. <b>B</b>. The three splicing isoforms FL, D11q and D11 and the position of specific oligos used for detection, are shown. <b>C</b>. Detection of BRCA1 exon 11 splicing isoforms for: _MCF7 endogenous BRCA1 (endogenous WT). _pB1 WT minigene transfected in MCF7 (pB1 WT). _pB1 minigene carrying the c.696G>A nucleotide substitution transfected in MCF7 (pB1 c.696G>A).</p

Concorde : le journal du groupe Air France

25 novembre 20042004/11/25 (N163)-2004/11/25

Additional file 6: Figure S6. of A role for endothelial nitric oxide synthase in intestinal stem cell proliferation and mesenchymal colorectal cancer

Kaplan–Meier plots from an analysis of the correlation between eNOS (a) or iNOS (b) mRNA expression level and patient survival using the best separation. eNOS upregulation and low iNOS expression significantly decrease the 5-year survival in colorectal cancer, colon adenocarcinoma and rectum adenocarcinoma patients. Patients were divided into low or high groups based on the level of expression of the NOS isoform. Data were taken from The Human Protein Atlas database. (TIF 76270 kb

Additional file 1: Figure S1. of A role for endothelial nitric oxide synthase in intestinal stem cell proliferation and mesenchymal colorectal cancer

Qualitative supervised classification of human CRC tumors into different CRC subtypes. Data analysis was performed using nSolver software from NanoString Technologies. Raw expression data were normalized and an overall average was established for each classifier gene, as described by Sadanandam et al. [10] and De Sousa et al. [6]. Positive expression indicates a value of expression above the average and it is shown as a green square, whereas negative expression is a value below the overall mean and it is shown as a red square. (TIF 64176 kb