22 research outputs found
Efficacy of a Novel Contact Pathway Inhibitor, Ir-CPI, on in vitro Clotting Induced by PCI Catheter Segment
Anticoagulation With an Inhibitor of Factors XIa and XIIa During Cardiopulmonary Bypass
peer reviewedBackground: Exposure of blood to polyanionic artificial surfaces, for example, during cardiopulmonary bypass (CPB), induces a highly procoagulant condition requiring strong anticoagulation. Unfractionated heparin (UFH) is currently used during CPB but can lead to serious bleeding complications or development of a hypercoagulable state culminating in life-threatening thrombosis, highlighting the need for safer antithrombotics. Ixodes ricinus contact phase inhibitor (Ir-CPI) is a protein expressed by I. ricinus ticks, which specifically inhibits both factors XIIa and XIa, 2 factors contributing to thrombotic disease while playing a limited role in hemostasis. Objectives: This study assessed the antithrombotic activity of Ir-CPI in animal contact phase-initiated thrombosis models, including CPB. The safety of Ir-CPI also was evaluated. Methods: The authors evaluated the antithrombotic activity of Ir-CPI by using in vitro catheter-induced clotting assays and rabbit experimental models of catheter occlusion and arteriovenous shunt. During CPB with cardiac surgery in sheep, the clinical applicability of Ir-CPI was investigated and its efficacy compared to that of UFH using an uncoated system suitable for adult therapy. Taking advantage of the similar hemostatic properties of pigs and humans, the authors performed pig liver bleeding assays to evaluate the safety of Ir-CPI. Results: Ir-CPI prevented clotting in catheter and arteriovenous shunt rabbit models. During CPB, Ir-CPI was as efficient as UFH in preventing clot formation within the extracorporeal circuit and maintained physiological parameters during and post-surgery. Unlike UFH, Ir-CPI did not promote bleeding. Conclusions: Preclinical animal models used in this study showed that Ir-CPI is an effective and safe antithrombotic agent that provides a clinically relevant approach to thrombosis prevention in bypass systems, including highly thrombogenic CPB. © 2019 The Author
Contribution à l'étude de la différenciation induite des cellules érythroleucémiques de Friend
Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe
Contribution à l'étude de la différenciation induite des cellules érythroleucémiques de Friend
Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe
Induction and properties of β-adrenergic receptors during erythroid differentiation of Friend erythroleukemic cells
SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe
Regulation of β-adrenergic receptors in Friend leukemic cells
SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Changes in adenylate cyclase activity during erythroid differentiation of Friend erythroleukemic cells
Significant changes in membrane-bound enzyme, adenylate cyclase were found in Friend erythroleukemic cells during erythroid differentiation induced by different chemical agents.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Characterization of beta-adrenergic receptors on Friend erythroleukemic cells [proceedings]
Journal Articleinfo:eu-repo/semantics/publishe
Regulation of β-adrenergic receptor number and coupling during chemically induced erythroid differentiation of Friend erythroleukemic cells
SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe