221 research outputs found
Unravelling the refolding of membrane proteins, one of the most challenging therapeutic targets
The intrinsically disordered DPF3 zinc finger protein: a promising new target in cancer therapy
The intrinsically disordered DPF3 zinc finger protein: a promising new target in cancer therapy
Endometrial osteoid metaplasia.
editorial reviewedEndometrial osteoid metaplasia is a rare condition characterised by the presence of bone in the uterine cavity. The main symptoms are secondary infertility, cycle disorders or dysmenorrhea. The discovery can also be fortuitous. Ultrasound and diagnostic hysteroscopy are diagnostic tools while operative hysteroscopy provides treatment. In the vast majority of cases, it is discovered in the aftermath of pregnancy, especially after curettage or miscarriage. There are several theories as to its etiopathogenesis.La métaplasie ostéoïde est définie par la présence de tissu osseux au niveau de l’endomètre. Il s’agit d’une affection rare. Les principaux symptômes sont l’infertilité secondaire, des troubles du cycle ou de la dysménorrhée. La découverte peut également être fortuite. L’échographie et l’hystéroscopie diagnostique sont des outils de diagnostic tandis que l’hystéroscopie opératoire assure le traitement. Dans la grande majorité des cas, elle est découverte dans les suites d’une grossesse, surtout après un curetage ou une fausse-couche. Il existe plusieurs théories quant à son étiopathogénie
Protecting role of cosolvents in protein denaturation by SDS: a structural study
<p>Abstract</p> <p>Background</p> <p>Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by addition of the amphipathic solvent 2,4-Methyl-2-PentaneDiol (MPD), used as protecting but also as refolding agent for these proteins. Although the persistence of the protein activity in the SDS/MPD mixture was clearly established, preservation of their structure was only speculative until now.</p> <p>Results</p> <p>In this paper, a detailed X-ray study addresses the pending question. Crystals of hen egg-white lysozyme were grown for the first time in the presence of MPD and denaturing concentrations of SDS. Depending on crystallization conditions, tetragonal crystals in complex with either SDS or MPD were collected. The conformation of both structures was very similar to the native lysozyme and the obtained complexes of SDS-lysozyme and MPD-lysozyme give some insights in the interplay of protein-SDS and protein-MPD interactions.</p> <p>Conclusion</p> <p>This study clearly established the preservation of the enzyme structure in a SDS/MPD mixture. It is hypothesized that high concentrations of MPD would change the properties of SDS and lower or avoid interactions between the denaturant and the protein. These structural data therefore support the hypothesis that MPD avoids disruption of the enzyme structure by SDS and can protect proteins from SDS denaturation.</p
Propriétés associées au désordre et mécanisme d'agrégation d'une nouvelle IDP amyloïdogène:la protéine à doigts de zinc DPF3a
Propriétés associées au désordre et mécanisme d'agrégation d'une nouvelle IDP amyloïdogène:la protéine à doigts de zinc DPF3a
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