3 research outputs found
Synthesis and Cytostatic and Antiviral Profiling of Thieno-Fused 7‑Deazapurine Ribonucleosides
Two
isomeric series of new thieno-fused 7-deazapurine ribonucleosides
(derived from 4-substituted thieno[2′,3′:4,5]pyrrolo[2,3-<i>d</i>]pyrimidines and thieno[3′,2′:4,5]pyrrolo[2,3-<i>d</i>]pyrimidines) were synthesized by a sequence involving
Negishi coupling of 4,6-dichloropyrimidine with iodothiophenes, nucleophilic
azidation, and cyclization of tetrazolopyrimidines, followed by glycosylation
and cross-couplings or nucleophilic substitutions at position 4. Most
nucleosides (from both isomeric series) exerted low micromolar or
submicromolar in vitro cytostatic activities against a broad panel
of cancer and leukemia cell lines and some antiviral activity against
HCV. The most active were the 6-methoxy, 6-methylsulfanyl, and 6-methyl
derivatives, which were highly active to cancer cells and less toxic
or nontoxic to fibroblasts
Prospective Evaluation of Free Energy Calculations for the Prioritization of Cathepsin L Inhibitors
Improving the binding affinity of
a chemical series by systematically
probing one of its exit vectors is a medicinal chemistry activity
that can benefit from molecular modeling input. Herein, we compare
the effectiveness of four approaches in prioritizing building blocks
with better potency: selection by a medicinal chemist, manual modeling,
docking followed by manual filtering, and free energy calculations
(FEP). Our study focused on identifying novel substituents for the
apolar S2 pocket of cathepsin L and was conducted entirely in a prospective
manner with synthesis and activity determination of 36 novel compounds.
We found that FEP selected compounds with improved affinity for 8
out of 10 picks compared to 1 out of 10 for the other approaches.
From this result and other additional analyses, we conclude that FEP
can be a useful approach to guide this type of medicinal chemistry
optimization once it has been validated for the system under consideration
Prospective Evaluation of Free Energy Calculations for the Prioritization of Cathepsin L Inhibitors
Improving the binding affinity of
a chemical series by systematically
probing one of its exit vectors is a medicinal chemistry activity
that can benefit from molecular modeling input. Herein, we compare
the effectiveness of four approaches in prioritizing building blocks
with better potency: selection by a medicinal chemist, manual modeling,
docking followed by manual filtering, and free energy calculations
(FEP). Our study focused on identifying novel substituents for the
apolar S2 pocket of cathepsin L and was conducted entirely in a prospective
manner with synthesis and activity determination of 36 novel compounds.
We found that FEP selected compounds with improved affinity for 8
out of 10 picks compared to 1 out of 10 for the other approaches.
From this result and other additional analyses, we conclude that FEP
can be a useful approach to guide this type of medicinal chemistry
optimization once it has been validated for the system under consideration