The Alder Hey Henoch Schonlein Purpura pathway - demonstrating follow up stratified according to the presence of proteinuria at the day 7 review.

<p>The Alder Hey Henoch Schonlein Purpura pathway - demonstrating follow up stratified according to the presence of proteinuria at the day 7 review.</p

The urinalysis (day 7) and the occurrence of a renal outcome.

<p>Each patient diagnosed with HSP had a random urinalysis taken and subsequent monitoring frequency determined on the presence or absence of proteinuria.</p

The HSP cohort - recruitment, follow up and outcome.

<p>The HSP cohort - recruitment, follow up and outcome.</p

Individual MRM traces obtained upon injection of processed DBS with blank blood into a column post-infused with MTXPG solutions; chromatograms indicated no matrix effect over the time of analysis.

<p>Individual MRM traces obtained upon injection of processed DBS with blank blood into a column post-infused with MTXPG solutions; chromatograms indicated no matrix effect over the time of analysis.</p

Results of the five calibration curves for MTXPGs; calibration points (nmol/L)^a, slope±SD, intercept and correlation coefficient of the standard curves.

a<p>Spiked concentrations of calibration standards [measured concentrations (mean±SD) are shown within brackets].</p

Representative MRM chromatograms of a blank DBS sample (A), DBS spiked with MTXPGs (final total concentration of 100 nM) subjected to enzymatic conversion to MTXPG1 (B), and DBS sample spiked with MTXPG1–5 measured individually at a final concentration of 100 nM each (C).

<p>Representative MRM chromatograms of a blank DBS sample (A), DBS spiked with MTXPGs (final total concentration of 100 nM) subjected to enzymatic conversion to MTXPG1 (B), and DBS sample spiked with MTXPG1–5 measured individually at a final concentration of 100 nM each (C).</p

Results of within day (intra-day) and between days (inter-day) accuracy and precision of MTXPG analysis (n = 5).

a<p>Relative standard deviation (RSD)% = [standard deviation (SD)/mean]×100.</p>b<p>Relative error (RE)% = [(measured−nominal)/nominal]×100.</p

Representative chromatograms of an extracted DBS sample obtained from JIA patient treated with MTX; (pg1–5): MRM chromatograms of individual MTXPGs; concentrations found were MTXPG₁, 9 nmol/L; MTXPG₂, 10 nmol/L; MTXP₃, 15 nmol/L; MTXPG₄, 10 nmol/L and MTXPG₅, 7 nmol/L, respectively.

<p>(MTX-total): Chromatogram after conversion of total MTXPGs to MTXPG₁; total concentration found was 49 nmol/L.</p

MRM parameters for the analysis of MTXPGs.

<p>MRM parameters for the analysis of MTXPGs.</p

Effect of varying blood haematocrit on the measured area of DBS spot formed (mean area ± SD; n = 8).

<p>Effect of varying blood haematocrit on the measured area of DBS spot formed (mean area ± SD; n = 8).</p