Self-Assembled DNA Nanostructures

<div><p>(A) DNA “tile” structure consisting of four branched junctions oriented at 90° intervals. These tiles serve as the primary “building block” for the assembly of the DNA nanogrids shown in (B). Each tile consists of nine DNA oligonucleotides as shown.</p> <p>(B) An atomic force microscope image of a self-assembled DNA nanogrid. Individual DNA tiles self-assemble into a highly ordered periodic two-dimensional DNA nanogrid.</p> <p>(Images were kindly provided by Thomas H. LaBean and Hao Yan.)</p></div

Additional file 2: Figure S1. of Novel miR-b2122 regulates several ALS-related RNA-binding proteins

3’UTR isoforms of RNA-binding proteins, and miR-194 and miR-b2122 are expressed in SH-SY5Y cells. (A) 3’RACE PCR showing TARDBP, FUS/TLS and RGNEF 3’UTR isoforms expressed in SH-SY5Y cells. FUS/TLS and RGNEF isoforms match those expressed in human spinal cord. TARDBP showed multiple isoforms, but only the 1398b isoform identified in spinal cord could be confirmed by sequencing. (B) Real-time PCR indicating the expression of miR-194 and miR-b2122 in SH-SY5Y cells. (TIFF 171 kb

Additional file 1: Table S1. of Novel miR-b2122 regulates several ALS-related RNA-binding proteins

Site-directed mutagenesis primers for TARDBP, FUS/TLS and RGNEF 3’UTRs. (DOCX 16 kb

Additional file 4: Figure S3. of Novel miR-b2122 regulates several ALS-related RNA-binding proteins

Let-7a has no effect on protein levels of TDP-43, FUS/TLS, or RGNEF within SH-SY5Y cells. Let-7a was transfected into SH-SY5Y cells to determine if it changed the basal protein levels of TDP-43, FUS/TLS or RGNEF, and was compared to a non-transfected control. The data indicated no significant change in the protein levels of either TDP-43 (p=0.71), FUS/TLS (p=0.28), or RGNEF (p=0.87) between the two conditions. Data is expressed as sample mean ± SEM, and significance was determined using a Student’s t-test. (TIFF 215 kb

Union of TB and NTM gene sets: network 1.

<p>Grey: Network identified genes; Blue: TB or NTM focus genes.</p

Network-identified immune-modulating chemicals.

<p>Grey: Network identified chemicals; Blue: Network identified genes.</p

Intersection of TB and NTM gene sets.

<p>Grey: Network identified genes; Blue: TB and NTM focus genes.</p

Database-derived TB and NTM-associated genes.

<p>Database-derived TB and NTM-associated genes.</p

TB network.

<p>Grey: Network identified genes; Blue: TB focus genes.</p

Network-identified nutrients.

<p>Grey: Network identified vitamins; Blue: Network identified genes.</p