2 research outputs found
Lipid-Mediated Targeting with Membrane-Wrapped Nanoparticles in the Presence of Corona Formation
Membrane-wrapped nanoparticles represent
a versatile platform for
utilizing specific lipid–receptor interactions, such as siallyllactose-mediated
binding of the ganglioside GM3 to Siglec1 (CD169), for targeting purposes.
The membrane wrap around the nanoparticles not only serves as a matrix
to incorporate GM3 as targeting moiety for antigen-presenting cells
but also offers unique opportunities for constructing a biomimetic
surface from lipids with potentially protein-repellent properties.
We characterize nonspecific protein adsorption (corona formation)
to membrane-wrapped nanoparticles with core diameters of approximately
35 and 80 nm and its effect on the GM3-mediated targeting efficacy
as a function of surface charge through combined <i>in vitro</i> and <i>in vivo</i> studies. The stability and fate of
the membrane wrap around the nanoparticles in a simulated biological
fluid and after uptake in CD169-expressing antigen-presenting cells
is experimentally tested. Finally, we demonstrate in hock immunization
studies in mice that GM3-decorated membrane-wrapped nanoparticles
achieve a selective enrichment in the peripheral regions of popliteal
lymph nodes that contain high concentrations of CD169-expressing antigen-presenting
cells
Fly Light Split-GAL4 Driver Collection
<p>The data presented on this site are the work of the <a href="http://janelia.org/team-project/fly-light" target="_blank">Janelia FlyLight Project Team</a> and the laboratories of <a href="http://www.janelia.org/lab/rubin-lab" target="_blank">Gerald M. Rubin</a>. </p><p>The split-GAL4 lines can be requested from the Janelia fly facility by performing a search and adding the desired lines to your cart. You will then be able to use the FlyBank website to tell us where to send them. For additional help ordering lines, please contact us at <a href="mailto:flybank.janelia.org">flybank.janelia.org</a></p><p>In publications, please attribute the data presented on this site to one of the following papers, as follows: <br><br>For the overall strategy and methods used to produce the split-GAL4 lines for the mushroom body neurons: <br>Aso, Y., Hattori, D., Yu, Y., Johnston, R. M., Iyer, N., Ngo, T. B., Dionne, H., Abbott, L. F., Axel, R., Tanimoto, H. & Rubin, G. M. . The neuronal architecture of the mushroom body provides a logic for associative learning. <a href="http://elifesciences.org/content/3/e04577" target="_blank">eLife (2014) 3:e04577</a><br><br>For split-GAL4 lines for the Lobula Columnar (LC) visual projection neurons:<br>Wu, M., Nern, A., Williamson, W. R., Morimoto, M. M., Reiser, M. B., Card, G. M. & Rubin, G. M. Visual projection neurons in the Drosophila lobula link feature detection to distinct behavioral programs. under review<br><br>For refinement of the split-GAL4 vectors and methodology: <br>Pfeiffer, B. D., Ngo, T. T., Hibbard, K. L., Murphy, C., Jenett, A., Truman, J. W. & Rubin, G. M. Refinement of tools for targeted gene expression in Drosophila. <a href="http://www.genetics.org/content/186/2/735.long" target="_blank">Genetics (2010) 186: 735-55</a>. <br><br>For Multicolor Flp-out (MCFO) technique and single cell labeling:<br>Nern, A., Pfeiffer, B.D., and Rubin, G.M. Optimized tools for multicolor stochastic labeling reveal diverse stereotyped cell arrangements in the fly visual system. <a href="http://www.pnas.org/content/112/22/E2967.long" target="_blank">Proc Natl Acad Sci USA (2015) 112: E2967-2976</a>. <br><br>Split-GAL4 lines were designed based on the expression patterns of GAL4 driver lines in the adult nervous system: <br>The Janelia collection of lines is described in Jenett, A., Rubin, G.M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H., Pfeiffer, B.D., Cavallaro, A., Hall, D., Jeter, J., Iyer, N., Fetter, D., Hausenfluck, J.H., Peng, H., Trautman, E., Svirskas, R., Myers, G.W., Iwinski, Z.R., Aso, Y., DePasquale, G.M., Enos, A., Hulamm, P., Lam, S.C.B., Li, H-H., Laverty, T., Long, F., Qu, L., Murphy, S.D., Rokicki, K., Safford, T., Shaw, K., Simpson, J.H., Sowell, A., Tae, S., Yu, Y., Zugates, C.T. A GAL4-Driver Line Resource for Drosophila Neurobiology. <a href="http://www.cell.com/cell-reports/fulltext/S2211-1247(12)00292-6" target="_blank">Cell Reports (2012) 2: 991-1001</a> <br><br>The VT collection of lines is described in Kvon, E.Z., Kazmar, T., Stampfel, G., Yanez-Cuna, J.O., Pagani, M., Schernhuber, K., Dickson, B.J., and Stark, A. Genome-scale functional characterization of Drosophila developmental enhancers in vivo. <a href="http://www.nature.com/nature/journal/vaop/ncurrent/full/nature13395.html" target="_blank">Nature (2014) 512: 91-95</a> and Barry J. Dickson, unpublished data. <br><br>For opening and viewing h5j and LSM stacks:<br>Use <a href="http://fiji.sc/" target="_blank">Fiji</a> (<a href="http://fiji.sc/" target="_blank">http://fiji.sc</a>). Fiji has a built-in plugin (H5J_Loader_Plugin-1.0.4) for opening stack in h5j format, a "visually lossless" compression format.</p