1 research outputs found
Injectable and Degradable Poly(Oligoethylene glycol methacrylate) Hydrogels with Tunable Charge Densities as Adhesive Peptide-Free Cell Scaffolds
Injectable,
dual-responsive, and degradable poly(oligo ethylene
glycol methacrylate) (POEGMA) hydrogels are demonstrated to offer
potential for cell delivery. Charged groups were incorporated into
hydrazide and aldehyde-functionalized thermoresponsive POEGMA gel
precursor polymers via the copolymerization of N,<i>N</i>′-dimethylaminoethyl methacrylate (DMAEMA) or acrylic acid
(AA) to create dual-temperature/pH-responsive in situ gelling hydrogels
that can be injected via narrow gauge needles. The incorporation of
charge significantly broadens the swelling, degradation, and rheological
profiles achievable with injectable POEGMA hydrogels without significantly
increasing nonspecific protein adsorption or chronic inflammatory
responses following in vivo subcutaneous injection. However, significantly
different cell responses are observed upon charge incorporation, with
charged gels significantly improving 3T3 mouse fibroblast cell adhesion
in 2D and successfully delivering viable and proliferating ARPE-19
human retinal epithelial cells via an “all-synthetic”
matrix that does not require the incorporation of cell-adhesive peptides