Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export-6

<p><b>Copyright information:</b></p><p>Taken from "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export"</p><p>http://www.retrovirology.com/content/4/1/95</p><p>Retrovirology 2007;4():95-95.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2234431.</p><p></p>mined by immunofluorescent microscopy in transfected 293 cells. Tax is expressed in nuclear foci in the absence of DNA damage (A) and is localized in the cytoplasm following UV irradiation (B). The addition of a UB tag at either the N- (C) or C- (E) termini caused Tax to be localized in the cytoplasm but treatment with LMB (D and F) sequestered UB-Tax and Tax-UB to nuclear foci. All images are shown at a magnification of 63×. The percentage of cells expressing nuclear foci (black bar) or cytoplasmic Tax (gray bar) was scored in five hundred transfected cells, in three independent experiments (G). Western blot showing expression of UB-Tax, Tax-UB, and native Tax proteins (H)

Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export-3

<p><b>Copyright information:</b></p><p>Taken from "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export"</p><p>http://www.retrovirology.com/content/4/1/95</p><p>Retrovirology 2007;4():95-95.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2234431.</p><p></p> HA-UB. Cells were either mock (-) or UV irradiated (+) and the ubiquitination status of Tax was examined by immunoblot analysis for Tax

Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export-4

<p><b>Copyright information:</b></p><p>Taken from "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export"</p><p>http://www.retrovirology.com/content/4/1/95</p><p>Retrovirology 2007;4():95-95.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2234431.</p><p></p>ed in 293 cells for 48 hrs and subjected to 30 J/mUV irradiation (B, D, F, H, J) or mock irradiated (A, C, E, G, I) and allowed to recover for 30 min. sc35 (left column) and Tax (left center column) were visualized by immunofluorescent microscopy separately, together (Merged, right center column), or together with DAPI (DAPI Merged, right column). All images are shown at a magnification of 63×

Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export-2

<p><b>Copyright information:</b></p><p>Taken from "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export"</p><p>http://www.retrovirology.com/content/4/1/95</p><p>Retrovirology 2007;4():95-95.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2234431.</p><p></p>bsence (black bars) or presence of 30 J/mUV irradiation (gray bars) is shown. Error bars represent the standard error in three independent experiments. (B) Western blot showing expression of each Tax mutant analyzed

Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export-1

<p><b>Copyright information:</b></p><p>Taken from "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export"</p><p>http://www.retrovirology.com/content/4/1/95</p><p>Retrovirology 2007;4():95-95.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2234431.</p><p></p>or UV irradiated (30 J/m, 30 minute recovery). The immunoprecipitates were analyzed by immunoblot for Tax (left) or HA (right)