2,592 research outputs found
Characterisation of a methyl-CpG binding domain protein in Caenorhabditis elegans and mammals
LAND RIGHTS AND INTRA-HOUSEHOLD EMPLOYMENT AND RESOURCE USE IN THE PERI-URBAN AREA OF BANJUL, THE GAMBIA
As part of cooperative research program examining factor markets in peri-urban areas of The Gambia (Banjul and Serekunda) to see if they are constraining agricultural growth and employment, particularly in the horticultural subsector, the household production survey reported in this study assesses inter- and intrahousehold issues of market access and constraints to land-improving investment and productivity. Implemented in 1993, survey is highly disaggregated by plot, gender, enterprise, and type of employment.Land tenure--Gambia--Banjul Region, Vendors and purchasers--Gambia--Banjul Region, Household production--Gambia, Research methods, Rural conditions--Gambia, Rural off-farm employment--Gambia, Income and wealth--Gambia, Land markets--Gambia, Tenure types, Traditional--Gambia, Labor and Human Capital, Land Economics/Use,
Pharmacology of airway afferent nerve activity
Afferent nerves in the airways serve to regulate breathing pattern, cough, and airway autonomic neural tone. Pharmacologic agents that influence afferent nerve activity can be subclassified into compounds that modulate activity by indirect means (e.g. bronchial smooth muscle spasmogens) and those that act directly on the nerves. Directly acting agents affect afferent nerve activity by interacting with various ion channels and receptors within the membrane of the afferent terminals. Whether by direct or indirect means, most compounds that enter the airspace will modify afferent nerve activity, and through this action alter airway physiology
Racial Disparities in Intravenous Recombinant Tissue Plasminogen Activator Use Persist at Primary Stroke Centers.
BACKGROUND: Primary stroke centers (PSCs) utilize more recombinant tissue plasminogen activator (rt-PA) than non-PSCs. The impact of PSCs on racial disparities in rt-PA use is unknown.
METHODS AND RESULTS: We used data from the Nationwide Inpatient Sample from 2004 to 2010, limited to states that publicly reported hospital identity and race. Hospitals certified as PSCs by The Joint Commission were identified. Adults with a diagnosis of ischemic stroke were analyzed. Rt-PA use was defined by the International Classification of Diseases, 9th Revision procedure code 99.10. Discharges (304 152 patients) from 26 states met eligibility criteria, and of these 71.5% were white, 15.0% black, 7.9% Hispanic, and 5.6% other. Overall, 24.7% of white, 27.4% of black, 16.2% of Hispanic, and 29.8% of other patients presented to PSCs. A higher proportion received rt-PA at PSCs than non-PSCs in all race/ethnic groups (white 7.6% versus 2.6%, black 4.8% versus 2.0%, Hispanic 7.1% versus 2.4%, other 7.2% versus 2.5%, all P
CONCLUSIONS: Racial disparities in intravenous rt-PA use were not reduced by presentation to PSCs. Black patients were less likely to receive thrombolytic treatment than white patients at both non-PSCs and PSCs. Hispanic patients were less likely to be seen at PSCs relative to white patients and were less likely to receive intravenous rt-PA in the fully adjusted model
Profound Learning and Living: An Exploratory Delphi Study
This study, using Delphi methodology conceptualizes the qualities of profound learning, the profound learner, and profound living
Science and the IS Researcher: Building an Empire Without Walls
The purpose of this paper is to introduce a radical alternative perspective into the debate on diversity, and the use of reference disciplines in IS research. It seeks to provide the foundation for a philosophical dialectic from which a new synthesis of the opposing views on the debate may emerge. Specifically, it is argued that the boundaries that divide academic disciplines are merely social conventions; products of convenience and individual and group self-interest. In contrast to this socially constructed view of scholarly inquiry, it is argued thatscience is a common good and that maturity of a field and of a researcher is evidenced not by defending the walls of a scientific empire but by contributing to the broader scientific community in which scholars in all fields may participate. The paradigm promulgated here is one of intellectual development and knowledge sharing that breaks down the walls of disciplines, views knowledge holistically, and considers the spread and evolution of ideas as the most important goal of all researchers. More than justa philosophical ideal, with the advent of the world wide web this new paradigm becomes a very real possibilit
Mass Spectrometry-based Methods for Phosphorylation Site Mapping of Hyperphosphorylated Proteins Applied to Net1, a Regulator of Exit from Mitosis in Yeast
Prior to anaphase in Saccharomyces cerevisiae, Cdc14 protein phosphatase is sequestered within the nucleolus and inhibited by Net1, a component of the RENT complex in budding yeast. During anaphase the RENT complex disassembles, allowing Cdc14 to migrate to the nucleus and cytoplasm where it catalyzes exit from mitosis. The mechanism of Cdc14 release appears to involve the polo-like kinase Cdc5, which is capable of promoting the dissociation of a recombinant Net1·Cdc14 complex in vitro by phosphorylation of Net1. We report here the phosphorylation site mapping of recombinant Net1 (Net1N) and a mutant Net1N allele (Net1N-19m) with 19 serines or threonines mutated to alanine. A variety of chromatographic and mass spectrometric-based strategies were used, including immobilized metal-affinity chromatography, alkaline phosphatase treatment, matrix-assisted laser-desorption post-source decay, and a multidimensional electrospray mass spectrometry-based approach. No one approach was able to identify all phosphopeptides in the tryptic digests of these proteins. Most notably, the presence of a basic residue near the phosphorylated residue significantly hampered the ability of alkaline phosphatase to hydrolyze the phosphate moiety. A major goal of research in proteomics is to identify all proteins and their interactions and post-translational modification states. The failure of any single method to identify all sites in highly phosphorylated Net1N, however, raises significant concerns about how feasible it is to map phosphorylation sites throughout the proteome using existing technologies
Conceptualizing An Abundance Mentality and Its Relationship to Lifelong Learning, Human Flourishing, and Profound Learning
The purpose of this exploratory literature review is to describe and continue to develop the concept of an abundance mentality and how it relates to lifelong learning, human flourishing, and profound learning
Genetic Variation in Clinical Varicella-Zoster Virus Isolates Collected in Ireland Between 2002 and 2003
Analysis of genetic variation in 16 varicella-zoster
virus (VZV) isolates selected at random and circulating in the Irish population between March 2002 and February 2003 was carried out. A 919 bp fragment of the glycoprotein E gene (open reading frame 68) encompassing codon 150, at which a non-synonymous mutation defines the escape
mutant VZV-MSP, and including two other epitope
regions e1 and c1, was sequenced. No new single nucleotide polymorphisms (SNPs) were detected, indicating stability of these epitopes in clinical isolates of VZV. However, when four informative polymorphic markers consisting of
defined regions from genes 1, 21, 50, and 54 were sequenced 14 variable nucleotide positions were identified. Phylogenetic analysis showed the presence of three highly supported clades A, B, and C circulating in the Irish population.
Approximately one third (6/16; 37.5%) of the Irish
VZV isolates in this study belonged to genotype
C, 4/16 (25%) to genotype A, and 4/16 (25%) to genotype B. A smaller number 2/16 (12.5%) belonged to genotype J1. This indicates remarkable heterogeneity in the Irish population given the small sample size. No evidence was found to
suggest any of the 16 isolates was a recombinant. These findings have implications for the model of geographic isolation of VZV clades to certain regions as the circulating Irish VZV population appears to comprise approximately equal numbers of each of the main genotypes. This data is inconsistent with a model of strict geographical separation of VZV genotypes and suggests that
VZVdiversity ismorepronounced in certain areas than had been thought previously
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