MTS viability assay performed with different concentrations of G-1, after 3 and 12 hours.

<p>Data was analyzed using two-way ANOVA with time and concentrations as factors.</p

Testosterone production in human testis biopsy after 3 hours.

<p>Experiments in humans were performed using 12 testis samples. All results were pooled in statistical analysis using one-way Anova.</p

Dose response analysis of testosterone production in rat Leydig cells with E2, G-1 and ICI incubation.

<p>Final concentration of 10, 50, 100 and 500-statistically significant difference between 10, 50 and 100 nM.</p

Localization studies of GPER-1 in rat testis.

<p>A. Immunohistochemistry imaging of adult rat testis. GPER-1 antibody is labeled in red. B. Immunofluorescence localization analysis of adult rat testis, with GPER-1 labeled in red, and nuclei conterstained with DAPI (blue). C. High resolution Z-stack imaging with 3D deconvolution obtained from each fluorescent channel using motorized microscope.</p

Testosterone production in isolated LH stimulated Leydig cells after 3 hours.

<p>Co incubation with G-1, E2, G-1 and E2.</p