4 research outputs found
S‑Nitroso-N-acetylpenicillamine (SNAP) Derivatization of Peptide Primary Amines to Create Inducible Nitric Oxide Donor Biomaterials
An
S-nitroso-N-acetylpenicillamine (SNAP) derivatization approach
was used to modify existing free primary amines found in fibrin (a
natural protein-based biomaterial) to generate a controlled nitric
oxide (NO) releasing scaffold material. The duration of the derivatization
reaction affects the NO release kinetics, the induction of controlled
NO-release, hydrophobicity, swelling behavior, elastic moduli, rheometric
character, and degradation behavior. These properties were quantified
to determine changes in fibrin hydrogels following covalent attachment
of SNAP. NO-releasing materials exhibited minimal cytotoxicity when
cultured with fibroblasts or osteoblasts. Cells maintained viability
and proliferative character on derivatized materials as demonstrated
by Live/Dead cell staining and counting. In addition, SNAP-derivatized
hydrogels exhibited an antimicrobial character indicative of NO-releasing
materials. SNAP derivatization of natural polymeric biomaterials containing
free primary amines offers a means to generate inducible NO-releasing
biomaterials for use as an antimicrobial and regenerative support
for tissue engineering
Unadjusted preventive behaviors used by adults to avoid catching or spreading influenza by sociodemographic characteristics.
<p>Unadjusted preventive behaviors used by adults to avoid catching or spreading influenza by sociodemographic characteristics.</p
Preventive behaviors used by adults as precautions taken to avoid catching influenza or spreading influenza.
<p>Preventive behaviors used by adults as precautions taken to avoid catching influenza or spreading influenza.</p