Impacts of Spatial Interpolation Methods on Daily Streamflow Predictions with SWAT

Precipitation is a significant input variable required in hydrological models such as the Soil & Water Assessment Tool (SWAT). The utilization of inaccurate precipitation data can result in the poor representation of the true hydrologic conditions of a catchment. SWAT utilizes the conventional nearest neighbor method in assigning weather parameters for each subbasin; a method inaccurate in representing spatial variations in precipitation over a large area, with sparse network of gauging stations. Therefore, this study aims to improve the spatial variation in precipitation data to improve daily streamflow simulation with SWAT, even pre-model calibration. The daily streamflow based on four interpolation methods, nearest neighbor (default), inverse-distance-weight, radial-basis function, and ordinary kriging, were evaluated to determine which interpolation method is best represents the precipitation at Yongdam watershed. Based on the results of this study, the application of spatial interpolation methods generally improved the performance of SWAT to simulate daily streamflow even pre-model calibration. In addition, no universal method can accurately represent the long-term spatial variation of precipitation at the Yongdam watershed. Instead, it was observed that the optimal selection of interpolation method at the Yongdam watershed is dependent on the long-term climatological conditions of the watershed. It was also observed that each interpolation method was optimal based on certain meteorological conditions at Yongdam watershed: nearest neighbor for cases when the occurrence probability of extreme precipitation is high during wet to moderately wet conditions; radial-basis function for cases when the number of dry days were high, during wet, severely dry, and extremely dry conditions; and ordinary kriging or inverse-weight-distance method for dry to moderately dry conditions. The methodology applied in this study improved the daily streamflow simulations at Yongdam watershed, even pre-model calibration of SWAT

Detailed Trend Analysis of Extreme Climate Indices in the Upper Geum River Basin

To investigate the recent effects of climate change in the upper Geum River basin in South Korea, a detailed trend analysis of 17 extreme climate indices based on 33 years (1988–2020) of daily precipitation, and daily (minimum and maximum) temperature data has been analyzed in this study. Out of the 17 extreme climate indices, nine (eight) indices were based on temperature (precipitation) data. Trend analysis based on detailed temporal scales (annual, seasonal, monthly) were performed through the Mann–Kendall trend test and the Theil–Sen slope method. Furthermore, the Mann–Whitney–Pettit test was also applied in this study, to detect abrupt changes in the extreme climate indices. Based on the results of this study, the climate conditions at the upper Geum River basin for the past three decades can be summarized as follows: general increase in temperature intensity, decrease in cold duration, increased heat duration, increased precipitation intensity, and increased consecutive wet and dry durations. Furthermore, a prolonged summer season (shorter spring, and autumn periods) and precipitation shifts, were detected based on trend analysis results of seasonal, and monthly time scales. The results presented in this study can provide supplementary data for improving watershed management strategies in the upper Geum River basin

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field