Relationships with God among Young Adults: Validating a Measurement Model with Four Dimensions

Experiencing a relationship with God is widely acknowledged as an important aspect of personal religiosity for both affiliated and unaffiliated young adults, but surprisingly few attempts have been made to develop measures appropriate to its latent, multidimensional quality. This paper presents a new model for measuring relationships with God based on religious role theory, attachment to God theory, and insights from interview-based studies, which allows for a wider array of dimensions than have been considered in prior work: anger, anxiety, intimacy, and consistency. To test our model's internal validity, we use confirmatory factor analysis with nationally representative data. To test its external validity, we (1) use difference-in-means tests across gender, race/ethnicity, geographical region, and religious affiliation; and (2) analyze correlations between our four new dimensions and four other commonly used measures of religiosity, thereby demonstrating both our model's validity and value for future studies of personal religiosity

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

HARNESS: a next generation distributed virtual machine

Heterogeneous Adaptable Reconfigurable Networked SystemS (HARNESS) is an experimental metacomputing system [L. Smarr, C.E. Catlett, Communications of the ACM 35 (6) (1992) 45–52] built around the services of a highly customizable and reconfigurable Distributed Virtual Machine (DVM). The successful experience of the HARNESS design team with the Parallel Virtual Machine (PVM) project has taught us both the features which make the DVM model so valuable to parallel programmers and the limitations imposed by the PVM design. HARNESS seeks to remove some of those limitations by taking a totally different approach to creating and modifying a DVM. ©1999 Elsevier Science B.V. All rights reserved. Keywords: Metacomputing; Message-passing library; Distributed application; Distributed virtual machine; PVM 1