Effect of a new biostimulant made by Fabaceae tissues on ripening dynamics and must technological main parameters in Vitis vinifera cv. Ribolla Gialla

Bio stimulants are organic compounds that influence biochemical activity within the treated plant, increasing the efficiency of the whole plant or any of its parts, yet can increase the uptake of necessary nutrients. Yet, they are very safe for humans, animals, and environment; thus, their use decreases the pollution caused by chemicals. From this point of view, recently, great attention has been focused on the possibility of using natural and safe stimulants in viticulture, which is one of the most cultivated and remunerative crop worldwide. Furthermore, protein hydrolysates (PHs) bio stimulants are an important group of such products based on a mixture of peptides and amino acids, that have received increasing attention in the recent years due to their positive effects on crop performances. Hence, the aim of this work was to investigate the foliar application of a bio stimulant developed by Ilsa SPA. This product is characterized by the presence of amino acids and peptides along with the presence of triacontanol of natural origin (> 6 mg / kg), saponins and various polyphenolic compounds (tannins, flavonoids, chlorogenic; gallic and cafferico acids). All of these have a bio stimulating and antioxidant action, naturally present in the plants of the Fabaceae family and which, thanks to the enzymatic hydrolysis process, are effectively extracted and made available for the plants. Thus, it was tested in order to study its effect of on the yield components, technological maturity trend, and its effect on some must parameters interesting for winemaking. This study was conducted during the season 2021 in a private vineyard of Ribolla Gialla grapevine (Vitis vinifera, L.) grafted onto rootstock SO4, in the north-east of Italy, Friuli Venezia Giulia region. The vines were 15- year-old, cultivated at 1 x 2 m, grown in clay soil without artificial irrigation system. The trellis system adopted was a trained single Guyot. In this region, the climate is generally high rainy (about 1800 mm/year), hence for farmers and winemakers is of great interest anticipating harvest time, to avoid phytoiatric problems, especially for those variety, as Ribolla Gialla, that tend to reach the technological maturity later than other variety, without compromising any agronomic and oenological parameters. After one-studied year, the natural bio stimulant had a positive action. In fact, data showed that ripening was anticipated in the treated plants (TT), having had higher parameters at every stage than the not treated (NT). In addition, we report how the TT treatment reached a full maturity for local standards one week earlier than NT. Finally, there also was a good effect on must microbiome main components, since the Yeasts present in the must was higher in TT, meaning that the proteins in the bio stimulant promoted the growth of the microbial community of berry skin

Effect of manure application timing on roots, canopy and must quality in Vitis vinifera 'Merlot': a case study in Italy, North-East

The maintenance and improvement of soil fertility are among the most important management practices in viticulture. The system efficiency fertilization (SEF) which is a new concept based on a maximum utilization of organic fertilizers (i.e., manure) has become very important, especially within the organic viticulture sector, since other fertilizers are not allowed. The aim of this study was to determine the effect of different manure application timing on the root, shoot, and the grapevine yield, accumulation, and quality of biochemical compounds in the grape must since the timing effect was not previously investigated. The study was carried out on 'Merlot' variety organically cultivated, whose production aims at obtaining high-quality red wines. Three treatments were applied: NT (Non-Treated), T1 (Treated1- manure applied in late October) and T2 (Treated2 - manure applied in late February). After two study-years, the undertaken research has shown positive influences of soil manure application on the canopy features (T1), yield, and yield components (T2), along with a major accumulation of the primary metabolites (T2) (soluble solid, carbohydrates, chlorophyll). Yet, the secondary metabolites (polyphenols and anthocyanins) were promoted in the grape must at harvest time, especially when the manure was applied in late October (T2). Considering the benefits of manure application in the T2, after two study years, this timing is recommended in order to improve 'Merlot' grapes for high-quality red wine production

In vitro application of Eruca vesicaria subsp. sativa leaf extracts and associated metabolites reduces the growth of Oomycota species involved in Kiwifruit Vine Decline Syndrome

This study aimed to determine whether leaf extracts from seven Eruca vesicaria subsp. sativa cultivars and their biochemical active compounds (glucosinolates and downstream-derived products) inhibit mycelia growth of three well-known pathogenic oomycetes, Phytopythium chamaehyphon, Phytopythium vexans and Phytophthora citrophthora; being the most significant in the development of Kiwifruit Vine Decline Syndrome (KVDS). Leaf extract quantity of 10, 20 and 30 mg were inoculated in Petri dish (90 mm Ø, each 22 mL of liquid medium – Potato Dextrose Agar), for in vitro bioassays. A pathogen plug was placed in the centre of each plate and the Oomycota colony perimeter was marked 5 days after inoculation. Radial colony growth was measured from 4 marks per plate 5, 10, and 15 days after inoculation, further elaborated with Image J software image analysis. Growth rates for all strains were inhibited by around 67% after 15 days. This was most pronounced when applying the highest concentration of leaf extract. By using Liquid Chromatography Mass Spectrometry (LC-MS) and Gas Chromatography Mass Spectrometry (GC-MS), fifteen glucosinolate compounds, of which glucosativin was found in the highest quantity, were identified. Concentrations of hydrolysis products produced by leaves (erucin and sativin) were also investigated, and were significantly associated with colony radial growth, especially towards Pp. chamaehyphon and Pp. vexans. Three downstream products of glucosinolates (two pure isothiocyanates, AITC and PEITC; and one indole I3C; all commonly present in Brassicaceae) were also tested, and a statistically significant inhibition of growth was observed at the highest concentration (0.6 µL)

Influence of Soil Chemical Features on Aromatic Profile of V. vinifera cv. Corvina Grapes and Wines: A Study-Case in Valpolicella Area (Italy) in a Calcareous and Non-Calcareous Soil

In the Valpolicella area (Verona, Italy) one of the most important Italian wines is being produced: Amarone, predominately made by Vitis vinifera cv. Corvina. This wine is subjected to a specific postharvest process, namely, withering, which aims to increase alcohol content and/or high residual sugars while retaining richness in aromas and organoleptic compounds. In accordance with guidelines and strict Amarone protocol set by the Consorzio of Amarone-Valpolicella, withering must be carried out by setting the grapes in a suitable environment. In our study, the withering process was performed following the traditional methods, i.e., in open-air, natural environmental conditions, as generally performed by winegrowers, leading to a weight loss in grapes of up to 30%. Whilst the effect of different postharvest conditions is clear and studied, it is interesting to know how soil can affect both grapes and wine profile, in terms of aromas, which are of great importance for this crafted wine. For this purpose, for two study years, the influence of two different sites (with regards to the carbonates’ content) on aromas were investigated. Furthermore, microvinifications and the sensory profile of the resulting wines were analysed. Our results clearly indicated that different soils’ characteristics, particularly differences in carbonate content, had an important effect, not only on the aromatic compounds of grapes and wines, but on the sensory profile as well. This information will help winegrowers and winemakers in the process of determining site selection for future vineyards in order to obtain a final optimum Amarone wine, in terms of its aromatic composition—one that is able to respond to the market demand

CTLA-4 and PD-1 ligand gene expression in epithelial thyroid cancers

The dysregulation of PD-1 ligands (PD-L1 and PD-L2) and CTLA-4 ligands (CD80 and CD86) represents a tumor strategy to escape the immune surveillance. Here, the expression of PD-L1, PD-L2, CD80 and CD86 was evaluated at mRNA level in 94 patients affected by papillary thyroid carcinoma (PTC) and 11 patients affected by anaplastic thyroid carcinoma (ATC). Variations in the mRNAs in PTC patients were then correlated with clinicopathological features. The expression of all genes was deregulated in PTC and ATC tissues compared to normal tissues. In particular, the down-regulation of CD80 was observed in above all ATC. In addition, the increased expression of CD80 associated to longer disease-free survival in PTC. Higher expression of PD-L1 associated with the classical histological variant and with the presence of BRAFV600E mutation in PTC. The increased PD-L2 expression correlated with BRAFV600E mutation and lymph node metastasis, while its lower expression correlated with the follicular PTC variant. The latter was also associated with the CD80 down-regulation, which was also related to the absence of lymph node metastasis. In conclusion, we documented the overall dysregulation of PD-1 and CTLA-4 ligands in PTC and ATC tissues and a possible prognostic value for CD80 gene expression in PTC