2,764 research outputs found
Preoperative Factors as Predictors of Outcome of Midurethral Sling in Women with Mixed Urinary Incontinence
Investigation of thermal resistance and power consumption in Ga-doped indium oxide (In2O3) nanowire phase change random access memory
The resistance stability and thermal resistance of phase change memory devices using similar to 40 nm diameter Ga-doped In2O3 nanowires (Ga:In2O3 NW) with different Ga-doping concentrations have been investigated. The estimated resistance stability (R(t)/R-0 ratio) improves with higher Ga concentration and is dependent on annealing temperature. The extracted thermal resistance (R-th) increases with higher Ga-concentration and thus the power consumption can be reduced by similar to 90% for the 11.5% Ga: In2O3 NW, compared to the 2.1% Ga: In2O3 NW. The excellent characteristics of Ga-doped In2O3 nanowire devices offer an avenue to develop low power and reliable phase change random access memory applications. (C) 2014 AIP Publishing LLC.X113sciescopu
Morphological Studies on Seeds of Scrophulariaceae s.l. and Their Systematic Significance
This study employed scanning electron microscopy and light microscopy to observe seed surface micromorphology and seed coat anatomy in the Scrophulariaceae s.l. to investigate seed characters of taxonomic importance. Seeds of 41 taxa corresponding to 13 genera of the family were carefully investigated. Seeds were minute and less than or slightly larger than 1 millimeter in length except for Melampyrum and Pedicularis species. The seed shape ranged from elliptical to broad elliptical and ovoid. In the studied species the surface sculpture was predominantly reticulate-striate, regular reticulate, sometimes colliculate, and rugose, or - rarely - ribbed, as in Lindernia procumbens and Paulownia coreana. Seed coats comprised the epidermis and the endothelium. Nevertheless, in all Melampyrum and some Veronica species the seed coat was very poorly represented and only formed by a papery layer of epidermis. According to correspondence analysis (CA) and unweighted pair group method with arithmetic mean (UPGMA) based cluster analysis the close affinities among the species of Scrophularia were well supported by their proximity to one another. Similarly, the proximity of Melampyrum species and Pedicularis species cannot be denied. In contrast, Veronica species were divided into two groups in CA plots and even three in the UPGMA tree. Regardless of the limited range taxa considered we found that similarities and differences in seed morphology between different genera could help us to understand the systematic relationships involved
Potassium chloride elicits enhancement of bilobalide and Ginkgolides production by Ginkgo biloba cell cultures
This study investigated the ability of potassium chloride (KCl) to elicit the production of bilobalide (BB), ginkgolide A (GA) and ginkgolide B (GB) by Ginkgo biloba cell suspension cultures. The salt stress by KCl treatments increased production of BB, GA and GB in both suspended cells and cultured medium. Especially, treatment of KCl 800 mM of highest concentration was stimulated emission into cultured medium BB, GA and GB compounds accumulated in cells. Although KCl 800 mM severely inhibited cells growth, the maximum content of GA and GB in cells was obtained in the treatment of KCl 800 mM, which was 1.9 and 4.0 times higher than the control. These results thus suggest that salt stress can afford enhanced production of secondary metabolites by plant cell cultures
An unusual cause of duodenal perforation due to a lollipop stick
Children have a natural tendency to explore objects with their mouths; this can result in the swallowing of foreign objects. Most ingested foreign bodies pass uneventfully through the gastrointestinal tract. However, some foreign bodies cause obstruction or perforation of the gastrointestinal tract, requiring surgical intervention. Perforation of the gastrointestinal tract may be associated with considerable morbidity and mortality. The most common sites of intestinal foreign body perforation are the ileocecal and rectosigmoid regions. Foreign body perforation of the duodenum is relatively uncommon. We report the first Korean case of duodenal perforation by an ingested 8-cm lollipop stick. Lollipops are popular with the children and fairly accessible to them, as most parents are not aware of their potential harm. Pediatric clinicians should be aware of the risks associated with lollipop stick ingestion. Our report also describes the feasibility and safety of laparoscopic diagnosis and management of pediatric patients with peritonitis induced by the ingestion of foreign bodies
Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors
<p>Abstract</p> <p>Background</p> <p>Caspase-3, a principal apoptotic effector that cleaves the majority of cellular substrates, is an important medicinal target for the treatment of cancers and neurodegenerative diseases. Large amounts of the protein are required for drug discovery research. However, previous efforts to express the full-length caspase-3 gene in <it>E. coli </it>have been unsuccessful.</p> <p>Results</p> <p>Overproducers of thrombin-activatable full-length caspase-3 precursors were prepared by engineering the auto-activation sites of caspase-3 precursor into a sequence susceptible to thrombin hydrolysis. The engineered precursors were highly expressed as soluble proteins in <it>E. coli </it>and easily purified by affinity chromatography, to levels of 10–15 mg from 1 L of <it>E. coli </it>culture, and readily activated by thrombin digestion. Kinetic evaluation disclosed that thrombin digestion enhanced catalytic activity (<it>k</it><sub>cat</sub>/<it>K</it><sub><it>M</it></sub>) of the precursor proteins by two orders of magnitude.</p> <p>Conclusion</p> <p>A novel method for a large-scale preparation of active caspase-3 was developed by a strategic engineering to lack auto-activation during expression with amino acid sequences susceptible to thrombin, facilitating high-level expression in <it>E. coli</it>. The precursor protein was easily purified and activated through specific cleavage at the engineered sites by thrombin, generating active caspase-3 in high yields.</p
Sound waves delay tomato fruit ripening by negatively regulating ethylene biosynthesis and signaling genes
AbstractRegulation of tomato fruit ripening may help extend fruit shelf life and prevent losses due to spoilage. Here, tomato fruit were investigated whether sound treatment could delay their ripening. Harvested fruit were treated with low-frequency sound waves (1kHz) for 6h, and then monitored various characteristics of the fruit over 14-days at 23±1°C. Seven days after the treatment, 85% of the treated fruit were green, versus fewer than 50% of the non-treated fruit. Most of the tomato fruit had transitioned to the red ripening stage by 14 days after treatment. Ethylene production and respiration rate were lower in the sound-treated than non-treated tomatoes. Furthermore, changes in surface color and flesh firmness were delayed in the treated fruit. To investigate how sound wave treatment effects on fruit ripening, the expression of ethylene-related genes was analyzed by quantitative real-time RT-PCR analysis. The expression level of several ethylene biosynthetic (ACS2, ACS4, ACO1, E4 and E8) and ripening-regulated (RIN, TAGL1, HB-1, NOR, CNR) genes was influenced by sound wave treatment. These results indicated that sound wave treatment delays tomato fruit ripening by altering the expression of important genes in the ethylene biosynthesis and ethylene signaling pathways
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