Quality Characteristics Of Wood Fish (Katsuobushi) Katsuo Arakamebushi And Katsuo Arahonbushi

Katsuobushi is a type of wood fish that has long been known to have a good flavor quality, usually used in traditional Japanese cuisine. Wood fish can also experience the process of damage, but when compared to other products the damage occurs more slowly as it is known that the low water content of the product can inhibit microbial activity. Basically, wood fish with good quality includes several processing processes including acceptance, boiling, extraction, bone, drying, and smoking. The selection of samples was taken randomly as many as 6 pieces of wooden fish products, the samples taken will be shaved and then will be in a blender and the prepared samples will be tested including analysis of water content, microbes, and organoleptic tests. The results obtained from the study were, skipjack tuna with sample code B2 had the best value in organoleptic color, loin shape, aroma, and texture by 15 semi-trained panelists. The moisture content of wooden skipjack tuna produced at PT. Celebes Minapratama meets the standards set by SNI. The results of the ALT study all samples of wood fish met the requirements because they did not exceed the specified limit. Keywords: Wood Fish, Smoking, Preservation. Abstrak Katsuobushi adalah jenis ikan kayu yang lama telah dikenal memiliki mutu flavor yang baik, biasanya digunakan dalam masakan tradisional jepang. Ikan kayu dapat juga mengalami proses kerusakan, namun bila dibandingkan dengan produk lain kerusakan yang terjadi lebih lambat seperti diketahui bahwa kadar air produk yang rendah dapat menghambat aktivitas mikroba. Pada dasarnya ikan kayu dengan kualitas baik yaitu meliputi beberapa proses pengolahan diantaranya penerimaan, perebusan, pencabutan, tulang, pengeringan dan pengasapan. Pemilihan sampel diambil secara acak sebanyak 6 potong produk ikan kayu, Sampel yang diambil akan di serut kemudian akan di blender dan sampel yang sudah disiapkan akan dilakukan pengujian diantarnya analisa kadar air, mikroba dan uji organoleptik. Hasil yang didapatkan  dari penelitian yaitu, Ikan cakalang kayu dengan kode sampel B2 memiliki nilai terbaik dalam organoleptik warna, bentuk loin, aroma dan tekstur oleh 15 panelis semi terlatih. Kadar air ikan cakalang kayu yang diproduksi di PT. Celebes Minapratama memenuhi standar yang ditetapkan SNI. hasil penelitian ALT semua sampel ikan kayu memenuhi syarat karena tidak melewati batas yang ditentukan. Kata Kunci: Ikan Kayu, Pengasapan, Pengaweta

Benzopyrene And moisture content In Wood Fish (Katsuobushi) Processed Using Conventional Smoking And Oven

Wooden fish is one type of processed fish product that has undergone a series of processes such as boiling and multilevel smoking until the texture becomes as hard as wood and dark brown-black in color. Usually, wooden fish uses skipjack tuna, but wood fish which is treated with smoke causes high levels of benzoic acid. a) pyrene in wood fish meat is high. The sample selection was taken as many as 2 pieces of wooden fish products, the samples taken would be shaved and then blended and the prepared samples would be tested including analysis of benzo(a)pyrene content and water content. The test method for benzo(a)pyrene uses HPLC and water content testing, using the Moisture Determination Balance FD-660, Analysis of benzo(a)pyrene levels and water content taken from the data of the last 4 years, trend data in research that has been carried out on levels of benzo(a) pyrene and water content, in the electric oven the highest content of benzo(a)pyrene in 2022 is 4.52 ppb and the highest water content in 2021 is 23.50% while conventional smoking has the highest benzo(a)pyrene content in 2019 of 56.6 ppb and the highest water content in 2020 by 19.40%. Testing the water content analysis of the moisture content in the oven is 22.50% and conventional smoking.is 19.40% Keywords: Wood Fish, Benzo(a)pyrene, Moisture Content, Smoking, Oven Abstrak Ikan kayu adalah salah satu jenis produk olahan ikan yang telah mengalami rangkaian proses seperti perebusan dan pengasapan bertingkat, hingga teksturnya menjadi sekeras kayu dan berwarna cokelat tua kehitaman. Biasanya olahan ikan kayu menggunakan ikan cakalang, akan tetapi ikan kayu yang diolah dengan asap menyebabkan kadar benzo(a)piren pada daging ikan kayu tinggi. Pemilihan sampel diambil sebanyak 2 potong produk ikan kayu sampel yang diambil akan diserut kemudian akan diblender dan sampel yang sudah disiapkan akan dilakukan pengujian diantaranya anilisa kadar benzo(a)piren dan kadar air. Metode pengujian benzo(a)piren menggunakan HPLC dan pengujian kadar air menggunakan alat Moisture Determination Balance FD-660, Analisa kadar benzo(a)piren dan kadar air diambil dari data 4 tahun terakhir. data trend pada penelitian yang telah dilakukan kadar benzo(a)piren dan kadar air, pada oven listrik paling tinggi kadar benzo(a)piren ditahun 2022 sebesar 4.52 ppb dan kadar air tertinggi ditahun 2021 sebesar 23.50% sedangkan pengasapan konvensional kadar benzo(a)piren paling tinggi ditahun 2019 sebesar 56.6 ppb dan kadar air tertinggi ditahun 2020 sebesar 19.40%. Pengujian analisa kadar air kadar air pada oven sebesar 22.50% dan pengasapan konvensional sebesar 19.40% Kata Kunci: Ikan Kayu, Benzo(a)piren, Kadar Air, Pengasapan, Oven

Studi In Silico Senyawa Aktif Daun Tagalolo (Ficus septica Burm F) sebagai Ligan Uji pada Enzim L-Histidin Decarboxylase

Histamine is one of the parameters in the tuna export trade that needs to be considered so that the product can be well received in the United States (USA), European Union (EU), and Japan. Histamine is used as a quality indicator for food safety of tuna products, because high histamine can cause toxic effects in humans. The leaves of the tagalolo/awar-awar plant (Ficus septica Burm. F) contain active compounds that function to cure several types of diseases such as ulcers, itching, wounds, diarrhea, dysentery and drugs to reduce fever and are used as inflammatory drugs. This study aims to determine the types of active compounds in tagalolo/awar-awar leaves from the literature search and their potential to react with the enzyme L-histidine decarboxylase through non-empirical research in silico. The compounds obtained from the literature search are Genistin, ß-amyrin, Pyrrolidine and Phenanthroindollizidine. These compounds were then tested In Silico with molecular docking. The results of the in silico study showed that the compounds Genistin, ß-amyrin, Pyrrolidine and Phenanthroindollizidine could not react with the L-histidine decarboxylase enzyme because all the tested ligands did not react or bind to the receptor.Keyword:     Tagalolo plant, Histamine, In Silico, Molecular Docking. Histamin merupakan salah satu parameter dalam perdagangan ekspor tuna yang perlu diperhatikan agar produk dapat diterima dengan baik di Amerika Serikat (USA), Uni Eropa (UE), maupun Jepang. Histamin dijadikan indikator mutu untuk keamanan pangan produk tuna, karena histamin yang tinggi dapat menyebabkan efek keracunan pada manusia. Daun tanaman tagalolo/awar-awar (Ficus septica Burm. F) mengandung senyawa aktif yang berfungsi menyembuhkan beberapa jenis penyakit seperti obat bisul, gatal-gatal, luka, diare, disentri dan obat untuk menurunkan demam serta digunakan sebagai obat radang atau inflamasi. Penelitian ini bertujuan untuk mengetahui jenis-jenis senyawa aktif daun tagalolo/awar-awar hasil penelusuran pustaka dan potensinya untuk bereaksinya dengan enzim L-histidin dekarboksilase melalui riset non-empirik secara in silico. Senyawa yang didapatkan dari penelusuran pustaka yaitu Genistin, β- amyrin, Pyrrolidine dan Phenanthroindollizidine. Senyawa-senyawa tersebut kemudian diuji secara In Silico dengan penambatan molekul (molecular docking). Hasil studi secara in silico menunjukkan bahwa senyawa Genistin, β- amyrin, Pyrrolidine dan Phenanthroindollizidine tidak dapat bereaksi dengan enzim L-histidin dekarbokasilase karena semua ligan uji tidak bereaksi atau terikat dengan reseptor.Kata kunci:  Tanaman Tagalolo, Histamin, In Silico, Molecular Docking

Identifikasi Vibrio SP pada Gonad Ikan Cakalang (Katsuwonus Pelamis L)

This research was conducted to identify the presence of Vibrio sp. at the gonads of fresh and smoked skipjack tuna. The method used in this research is descriptive method with the parameters examined, ie total bacteria, total Vibrio, morphology and biochemical tests. Results showed that the total value of Vibrio in fresh skipjack tuna gonad highest TVC 1.1x105/g and the lowest is 2.0x103 TVC/gr. While the smoke skipjack tuna gonad bacterial growth does not occur. The identification results found 43 strains of Vibrio sp., (7 strains), Vibrio fluvialis (2 strains), Vibrio cincinnatiensis (4 strains), Vibrio damsela (2 strains), Vibrio metschnikovii (1 strain) and 27 other strains was not identified

Mold On Cakalang Fish (Katsuwonus pelamis L) Smoke With Various Packaging In The Supermarket

This research aims to obtain total mold and identify the types of mold found in smoked skipjack tuna (Katsuwonus pelamis L) which is marketed in supermarkets with various packaging. The method used in this research is the descriptive method. The sample used was smoked skipjack tuna (Katsuwonus pelamis L) packaged in styrofoam, plastic, and wrapping. The parameters used are mold, water content, and pH. The results showed that the lowest total mold was found in samples of smoked skipjack tuna (Katsuwonus pelamis L) with plastic packaging. The water content of the three samples of smoked skipjack tuna (Katsuwonus pelamis L) in various packaging at supermarkets still met SNI 2725:2013 standards. The pH of smoked skipjack tuna (Katsuwonus pelamis L) in plastic packaging at the first collection did not meet the quality requirements. Keywords: smoked skipjack tuna, packaging, mold Abstrak Penelitian ini bertujuan untuk mendapatkan total kapang dan mengidentifikasikan jenis-jenis kapang yang terdapat pada ikan cakalang (Katsuwonus pelamis L) asap yang dipasarkan di pasar swalayan dengan berbagai pengemasan. Metode yang digunakan dalam penelitian ini adalah metode deskriptif. Sampel yang digunakan adalah ikan cakalang (Katsuwonus pelamis L) asap dengan pengemasan styrofoam, plastik dan wrapping. Parameter yang digunakan adalah kapang, kadar air dan pH. Hasil penelitian menunjukkan bahwa total kapang terendah terdapat pada sampel ikan cakalang (Katsuwonus pelamis L) asap  dengan kemasan plastik. Kadar air ketiga sampel ikan cakalang (Katsuwonus pelamis L) asap dengan berbagai pengemasan di Pasar Swalayan masih memenuhi standar SNI 2725:2013. pH ikan cakalang (Katsuwonus pelamis L) asap dengan kemasan plastik pada pengambilan pertama tidak memenuhi syarat mutu.perebusan 58.9% – 62.3% dan pada produk akhir ikan kayu 16.9 – 18.3%. ALT ikan segar 5.1 x 104 koloni/g – 6.5 x 104 koloni/g, setelah mengalami proses perebusan 3.0 x 103 koloni/g – 1.0 x 104 koloni/g dan pada produk akhir ikan kayu 2.5 x 102 koloni/g – 4.0 x 102 koloni/g. Total kapang pada ikan kayu hasilnya negatif. Kata kunci: cakalang asap, pengemasan, kapan

Quality Characteristics Of Wood Fish (Katsuobushi) Katsuo Arakamebushi And Katsuo Arahonbushi

Katsuobushi is a type of wood fish that has long been known to have a good flavor quality, usually used in traditional Japanese cuisine. Wood fish can also experience the process of damage, but when compared to other products the damage occurs more slowly as it is known that the low water content of the product can inhibit microbial activity. Basically, wood fish with good quality includes several processing processes including acceptance, boiling, extraction, bone, drying and smoking. The selection of samples was taken randomly as many as 6 pieces of wooden fish products, the samples taken will be shaved then will be in a blender and the prepared samples will be tested including analysis of water content, microbes and organoleptic tests. The results obtained from the study were, skipjack tuna with sample code B2 had the best value in organoleptic color, loin shape, aroma and texture by 15 semi-trained panelists. The moisture content of wooden skipjack tuna produced at PT. Celebes Minapratama meets the standards set by SNI. The results of the ALT study all samples of wood fish met the requirements because they did not exceed the specified limit.Keywords: Wood Fish, Smoking, Preservation.AbstrakKatsuobushi adalah jenis ikan kayu yang lama telah dikenal memiliki mutu flavor yang baik, biasanya digunakan dalam masakan tradisional jepang. Ikan kayu dapat juga mengalami proses kerusakan, namun bila dibandingkan dengan produk lain kerusakan yang terjadi lebih lambat seperti diketahui bahwa kadar air produk yang rendah dapat menghambat aktivitas mikroba. Pada dasarnya ikan kayu dengan kualitas baik yaitu meliputi beberapa proses pengolahan diantaranya penerimaan, perebusan, pencabutan, tulang, pengeringan dan pengasapan. Pemilihan sampel diambil secara acak sebanyak 6 potong produk ikan kayu, Sampel yang diambil akan di serut kemudian akan di blender dan sampel yang sudah disiapkan akan dilakukan pengujian diantarnya analisa kadar air, mikroba dan uji organoleptik. Hasil yang didapatkan  dari penelitian yaitu, Ikan cakalang kayu dengan kode sampel B2 memiliki nilai terbaik dalam organoleptik warna, bentuk loin, aroma dan tekstur oleh 15 panelis semi terlatih. Kadar air ikan cakalang kayu yang diproduksi di PT. Celebes Minapratama memenuhi standar yang ditetapkan SNI. hasil penelitian ALT semua sampel ikan kayu memenuhi syarat karena tidak melewati batas yang ditentukan.Kata Kunci: Ikan Kayu, Pengasapan, Pengaweta

Benzopyrene And Moisture Content In Wood Fish (Katsuobushi) Processed Using The Conventional Smoking And Oven

Wooden fish is one type of processed fish product that has undergone a series of processes such as boiling and multilevel smoking until the texture becomes as hard as wood and dark brown-black in color. Usually, wooden fish uses skipjack tuna, but wood fish which is treated with smoke causes high levels of benzoic acid. a) pyrene in wood fish meat is high. The sample selection was taken as many as 2 pieces of wooden fish products, the samples taken would be shaved and then blended and the prepared samples would be tested including analysis of benzo(a)pyrene content and water content. The test method for benzo(a)pyrene uses HPLC and water content testing, using the Moisture Determination Balance FD-660, Analysis of benzo(a)pyrene levels and water content taken from the data of the last 4 years, trend data in research that has been carried out on levels of benzo(a) pyrene and water content, in the electric oven the highest content of benzo(a)pyrene in 2022 is 4.52 ppb and the highest water content in 2021 is 23.50% while conventional smoking has the highest benzo(a)pyrene content in 2019 of 56.6 ppb and the highest water content in 2020 by 19.40%. Testing the water content analysis of the moisture content in the oven is 22.50% and conventional smoking.is 19.40%Keywords: Wood Fish, Benzo(a)pyrene, Moisture Content, Smoking, OvenAbstrakIkan kayu adalah salah satu jenis produk olahan ikan yang telah mengalami rangkaian proses seperti perebusan dan pengasapan bertingkat, hingga teksturnya menjadi sekeras kayu dan berwarna cokelat tua kehitaman. Biasanya olahan ikan kayu menggunakan ikan cakalang, akan tetapi ikan kayu yang diolah dengan asap menyebabkan kadar benzo(a)piren pada daging ikan kayu tinggi. Pemilihan sampel diambil sebanyak 2 potong produk ikan kayu sampel yang diambil akan diserut kemudian akan diblender dan sampel yang sudah disiapkan akan dilakukan pengujian diantaranya anilisa kadar benzo(a)piren dan kadar air. Metode pengujian benzo(a)piren menggunakan HPLC dan pengujian kadar air menggunakan alat Moisture Determination Balance FD-660, Analisa kadar benzo(a)piren dan kadar air diambil dari data 4 tahun terakhir. data trend pada penelitian yang telah dilakukan kadar benzo(a)piren dan kadar air, pada oven listrik paling tinggi kadar benzo(a)piren ditahun 2022 sebesar 4.52 ppb dan kadar air tertinggi ditahun 2021 sebesar 23.50% sedangkan pengasapan konvensional kadar benzo(a)piren paling tinggi ditahun 2019 sebesar 56.6 ppb dan kadar air tertinggi ditahun 2020 sebesar 19.40%. Pengujian analisa kadar air kadar air pada oven sebesar 22.50% dan pengasapan konvensional sebesar 19.40%Kata Kunci: Ikan Kayu, Benzo(a)piren, Kadar Air, Pengasapan, Oven