The World Health Organisation Classification Of Myelodysplastic Syndromes Contains Prognostically Relevant Information Beyond The Prognostic Scores Ipss-r Or Wpss

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Melhorando o diagnóstico diferencial entre síndromes mielodisplásicas e citopenias periféricas reativas por citometria de fluxo multiparamétrica: o papel dos precursores de células B

Immunophenotyping is a valuable ancillary technique for the differential diagnosis between myelodysplastic syndromes (MDS) with low bone marrow (BM) blast counts and a normal karyotype, and reactive peripheral (PB) cytopenias. Our aim was to search for the most important variables for this purpose. We also analyzed the age variation of BM B-cell precursors (BCP) and its differences in reactive and clonal cytopenias. Immunophenotypic analyzes were performed in BM of 54 patients with MDS (76% with BM blasts 55 years. % BM BCP could be calculated by the formula: (-7.97 × log age) + (4.24 × log % CD34 (+) cells) - (0.22 x nr. alterations CD34 (+) cells) + 0.577. Corrected R(2) = 0.467. Analysis of myelomonocytic precursors and CD34(+) cells was satisfactory for the differential diagnosis between reactive PB cytopenias and MDS. The most specific alterations were found in CD34(+) cells. Comparison of the values obtained with those of normal age-matched controls is recommended. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1975931809154663.Immunophenotyping is a valuable ancillary technique for the differential diagnosis between myelodysplastic syndromes (MDS) with low bone marrow (BM) blast counts and a normal karyotype, and reactive peripheral (PB) cytopenias. Our aim was to search for th104419FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO25924846sem informaçãoFAEPEX (proc 1208/11 Fundo de Pesquisa da Universidade de Campinas) e Fundação MDS (concessão de Jovem Investigador Tito Bastianello 2009). Konradin Metze e Irene Lorand-Metze têm uma bolsa de pesquisa do CNPq (proc. 307270 / 2010-6 e 302277 / 2009-9, re

Umbilical cord blood cd34(+) stem cells and other mononuclear cell subtypes processed up to 96 h from collection and stored at room temperature maintain a satisfactory functionality for cell therapy

Background and ObjectivesUmbilical cord blood (UCB) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (MNCs) subtypes were viable and functional until 96h after collection, even stored at room temperature. Now, we analyzed the viability and functionality of the cells before and after cryopreservation. Materials and MethodsTwenty UCB units were analyzed at 24 and 96h after collection, frozen for 6months, thawed and re-evaluated. MNCs were analyzed by flow cytometry, viability by 7-AAD and clonogenic assays (CFU) were performed. ResultsAfter 96h of storage, no substantial loss of MNC was found (median 7320x10(6)x6305x10(6)). Percentage and viability CD34(+) cells, B-cell precursors and mesenchymal stem cells were not affected. However, mature B and T lymphocytes as well as granulocytes had a substantial loss. CFU growth was observed in all samples. Prefreezing storage of 96h was associated with a relative loss of colony formation (median 12%). Postthaw, this loss had a median of 49% (24h samples) to 56% (96h samples). ConclusionThe delay of 96h before UCB processing is possible, without a prohibitive impairment of CD34(+) loss in number and functionality.Umbilical cord blood (UCB) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (MNCs) subtypes were viable and functional until 96h after collection, even stored at room temperature. Now, we analyzed the10817281sem informaçãosem informaçã

The utility of fluorescence lifetime imaging in routine bone marrow

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Clinical outcomes of patients with acute myeloid leukemia: evaluation of genetic and molecular findings in a real-life setting

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Presence of b-cell precursors in bone marrow is a favorable independent prognostic factor for overall survival in patients with myelodysplastic syndromes

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Monocyte phenotypic aberrancies are an unfavorable prognostic factor in patients with myelodysplastic syndromes and low ipss-r scores

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Fluorescence lifetime imaging in routine cytology

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The importance of immunophenotyping of hemopoietic precursors in the diagnosis of childhood mds

sem informação1011503504sem informaçãosem informação21st Congress of the European-Hematology-Associatio

Characteristics of the phenotypic abnormalities of bone marrow cells in childhoodmyelodysplastic syndromes and juvenilemyelomonocytic leukemia

Immunophenotyping of bone marrow (BM) hemopoietic precursors is useful for diagnosis of adult myelodysplastic syndrome (MDS), but data concerning pediatric patients are limited. We analyzed immunophenotypic features of BM cells at diagnosis of children who were referred to the Brazilian Pediatric Cooperative Group of Myelodysplastic Syndromes. Methods: Diagnosis was based on clinical information, peripheral blood counts, BM cytology and cytogenetics. Patients with Down syndrome were excluded. Children with deficiency anemias or transitory neutropenias were used as controls (CTRLs). Immunophenotyping was performed on an eight-color antibody platform evaluatingmyelomonocytic maturation and progenitor cells. Results: Atotal of 32 patients were examined: 6 refractory cytopenia of childhood [RCC]; 5 refractory anemia with excess of blasts [RAEB]; 8 refractory anemia with excess of blasts in transformation [RAEB-t]; 13 juvenile myelomonocytic leukemia [JMML] and 10 CTRLs. Median age was 66 months (RCC), 68 months (RAEB/RAEB-t), 29 months (JMML) and 70 months (CTRLs). Median number of phenotypic alterations was 4 (range 1-6) in RCC; 6 (range 2-11) in RAEB/RAEB-t and 6 (range 2-11) in JMML (P = 0.004). The percentage of CD34(+)/CD117(+)/CD13(+) cells was 0.5% (range 0.1-2.8) in RCC; 4.2% (range 0.3-10.1) in RAEB/RAEB-t and 3.7 % (range 0.5-8.6) in JMML cases, compared with 0.7% (0.5-1.2) in CTRLs (P < 0.0005). Aberrancies in antigen expression of myeloid progenitors were seen in 63% of JMML and in 45% of RAEB/RAEB-t. CD34(+)/CD19(+)/CD10(+) cells were decreased or absent in patients compared with age-matched controls. T lymphocytes were decreased in JMML. Conclusions: Phenotypic abnormalities were similar to those found in adult MDS. A decrease in B-cell precursors was observed especially in RAEB/RAEB-t. JMML and RAEB showed a similar pattern.Immunophenotyping of bone marrow (BM) hemopoietic precursors is useful for diagnosis of adult myelodysplastic syndrome (MDS), but data concerning pediatric patients are limited. We analyzed immunophenotypic features of BM cells at diagnosis of children wh64417sem informaçãosem informaçãoThe authors would like to acknowledge the Barretos Pediatric Can-cer Center, Brazil, for their financial support. Drs. Konradin Metze andIrene Lorand-Metze have a research grant from the Brazilian ResearchCouncil. All the patients in this study had a diag