31 research outputs found
Circular Dichroism Studies on C-terminal Zinc Finger Domain of Transcription Factor GATA-2
Get PDFThe C-terminal zinc finger domain of GATA-2 transcription factor from Rattus norvegicus has been expressed and purified to elucidate its secondary structure using circular dichroism spectroscopy. Circular dichroism spectra showed that native GATA-2 C-terminal domain of (Cys)4 type zinc finger has 12% α-helix, 36% β-sheet and 52% random coil content. The estimated structure was compared with predicted structures by sequence based prediction software SOPMA and found to be similar. Furthermore, the effect of pH on the secondary structure of GATA-2 C-finger was examined. This study provides an insight into the understanding of the structure and function of transcription factor GATA-2
Spectral analysis of the successive degradation process of kraft lignin by alkaline oxygen treatment
No full textHigh-resolution nuclear magnetic resonance spectroscopic study of metabolites in the cerebrospinal fluid of patients with cervical myelopathy and lumbar radiculopathy
No full textNuclear magnetic resonance studies of the role of histidine residues at the active site of rabbit muscle creatine kinase
No full textEthanol contamination of cerebrospinal fluid during standardized sampling and its effect on 1H-NMR metabolomics
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