Example of coccoliths derived at the end of the experiment (<i>t2</i>).

<p>Panel A: typical coccoliths from 395 p.p.m.v. CO₂ treatment; B: coccoliths from the lower pH (∼7.6) and 1340 p.p.m.v. CO₂ treatment which are typically larger and are slighly less calcified but possess no signs of dissolution or malformation.</p

Proteins (Homolgous Protein Groups) down-regulated in <i>Emilania huxleyi</i> NZEH under high CO₂.

a<p>Identifications are associated with either UniProtKB accession, <i>Emiliania huxleyi</i> genome protein ID or BUDAPEST consensus sequence. Further information can be found in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#pone.0061868.s002" target="_blank">Data S2</a>;</b> BUDAPEST sequence files can be found in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#pone.0061868.s001" target="_blank">Data S1</a></b>.</p>b<p>Ratio of protein identified between the two CO₂ treatments for each replicate incubation, whereby 114∶113 is the first replicate, 116∶115 is the second and 118∶117 the third. Reporter ions 114, 116 and 118 were applied to peptides extracted from the high CO₂ treatments and 113, 115, 117 to the current day treatment.</p>c<p>“Range” (<i>G</i>−<i>CI</i>)–(<i>G</i>+<i>CI</i>) as defined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#s2" target="_blank">Materials and Methods</a>.</p

Morphometric analysis of detached coccoliths obtained at the end of the experiment (<i>t2</i>) from monoclonal cultures of <i>Emiliania huxleyi</i> NZEH bubbled with 395 and 1340 p.p.m.v. CO_2.

<p>Morphometric analysis of detached coccoliths obtained at the end of the experiment (<i>t2</i>) from monoclonal cultures of <i>Emiliania huxleyi</i> NZEH bubbled with 395 and 1340 p.p.m.v. CO_2.</p

Proteins (Homologous Protein Groups) exhibiting non-significant expressional change in <i>Emilania huxleyi</i> NZEH under high CO₂.

a, b, c<p>As defined for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#pone-0061868-t003" target="_blank">table 3</a>.</p>d<p>If an identification was made from numerous ESTs with valid iTRAQ quantification, an geometric average has been made (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#s2" target="_blank">Materials and Methods</a>). Please refer to <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#pone.0061868.s002" target="_blank">Data S2</a></b> for individual EST quantification.</p

Mean carbonate chemistry values associated with experimental cultures (<i>t2</i>).

a<p>Average values at the beginning of the experiment before the inoculation of cells.</p>b<p>Figures in parentheses represent blank seawater medium bubbled with the appropriate <i>p</i>CO₂ mixture.</p>c<p>There was only one sample available for the initial pre-inoculation blank of the ambient/current day CO₂ condition. For this instance alone, values in parentheses are from the second 395 p.p.m.v. CO₂ treatment.</p>d<p>Average values at the end of the experiment (<i>t2</i>).</p

Physiological parameters of <i>Emiliania huxleyi</i> NZEH grown under 395 and 1340 p.p.m.v. CO₂ at <i>t1</i> and <i>2</i>.

*, **, ***<p>Significant results from t-tests comparing results from different <i>p</i>CO₂ treatments at the same time point (i.e. 395 v 1340 p.p.m.v. CO₂ at <i>t1</i>; 395 v. 1340 p.p.m.v. CO₂ at <i>t2</i>) are designated as follows: ^* p≤0.05; ^** p≤0.01; ^*** p≤0.001.</p>a, b,<p>Significant results from t-tests comparing results from the same <i>p</i>CO₂ treatment at different time points (e.g. 395 <i>t1</i> v 395 p.p.m.v. CO₂<i>t2</i>) are defined according to: ^a p≤0.05; ^b p≤0.01.</p

Comparison of <i>Emiliania huxleyi</i> NZEH coccosphere sizes at 395 and 1340 p.p.m.v. CO₂.

<p>* p≤0.05; ** p≤0.01; *** p≤0.001. A point with no star indicates differences were non-significant. Arrows indicate inoculations into media with different <i>p</i>CO₂ conditions, as outlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061868#s2" target="_blank">Materials and Methods</a>. Open circles represent cells grown under under 395 p.p.m.v. CO₂ that were harvested after 12–13 generations (<i>t2</i> = day 8). Solid circles indicate cells grown under 1340 p.p.m.v. CO₂. In order to ensure suitable biomass for proteomics, these were harvested after 9–12 generations (<i>t2</i> = day 9 or 10) because of their lower growth rates.</p