Anadolu Mandalarında Q Fever Hastalığının Serolojik Olarak Araştırılması

Buffaloes as in other animals have been demonstrated to play a role in certain diseases transmitted to susceptible animalsand human populations. In this study, serum samples were collected from Anatolian Buffaloes in breeding Samsun and around were examinedfor Q Fever. For this purpose, 184 sera were analyzed with commercial ELISA test kit. Totally 29 (15.8%) were determined positive for Q Feverfrom examined 184 serum samples. Serum samples obtained from Anatolian Buffaloes were examined first time in terms of serologically in ourregion respect to Q Fever. As a result, the data provided within the scope of the research indicate a Q Fever seropositivity level that could pose arisk for our indigenous buffalo population. We concluded that the data obtained from this study can constitute a resource to similar studies in ourregion. The epidemiology of the disease can be elaborated in the light of studies that will be carried out with more comprehensive researches inour region.Mandaların, diğer hayvanlarda olduğu gibi bazı hastalıkların duyarlı hayvan popülasyonlarına ve insanlara bulaştırılmasında rol oynadıkları ortaya konulmuştur. Bu çalışmada, Samsun ili ve ilçelerinde yetiştiriciliği yapılan Anadolu Mandalarına ait kan serum örnekleri Q fever hastalığı yönünden incelendi. Bu amaçla, 184 kan serumu ticari bir ELISA kiti ile test edildi. İncelenen 184 serum örneğinin 29 (%15.8)’u Q fever hastalığı açısından seropozitif bulundu. Bu araştırma ile bölgemizde ilk kez Anadolu Mandalarına ait serum örnekleri Q fever yönünden serolojik olarak incelendi. Sonuç olarak, proje kapsamında sağlanan veriler bölgemiz manda populasyonu için risk oluşturabilecek düzeyde Q fever seropozitifliğine işaret etmektedir. Yürütülen araştırmadan elde edilen verilerin, yöremizde yapılacak benzer çalışmalara kaynak teşkil edebileceği kanısına varıldı. Bölgemizde daha kapsamlı projelerle gerçekleştirilecek araştırmalar ışığında hastalığın epidemiyolojisi detaylı bir şekilde ortaya konulabilecektir

CD4(+) T cells of myasthenia gravis patients are characterized by ıncreased IL-21, IL-4, and IL-17A productions and higher presence of PD-1 and ICOS

Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies predominantly against the acetylcholine receptor (AChR). Specific T cell subsets are required for long-term antibody responses, and cytokines secreted mainly from CD4(+) T cells regulate B cell antibody production. The aim of this study was to assess the differences in the cytokine expressions of CD4(+) T cells in MG patients with AChR antibodies (AChR-MG) and the effect of immunosuppressive (IS) therapy on cytokine activity and to test these findings also in MG patients without detectable antibodies (SN-MG). Clinically diagnosed AChR-MG and SN-MG patients were included. The AChR-MG patients were grouped as IS-positive and -negative and compared with age- and sex-matched healthy controls. Peripheral blood mononuclear cells were used for ex vivo intracellular cytokine production, and subsets of CD4(+) T cells and circulating follicular helper T (cTfh) cells were detected phenotypically by the expression of the chemokine and the costimulatory receptors. Thymocytes obtained from patients who had thymectomy were also analyzed. IL-21, IL-4, IL-10, and IL-17A productions in CD4(+) T cells were increased in AChR-MG compared to those in healthy controls. IS treatment enhanced IL-10 and reduced IFN-gamma production in AChR-MG patients compared to those in IS-negative patients. Increased IL-21 and IL-4 productions were also demonstrated in SN-MG patients. Among CD4(+) T cells, Th17 cells were increased in both disease subgroups. Treatment induced higher proportions of Th2 cells in AChR-MG patients. Both CXCR5(+) and CXCR5(-) CD4(+) T cells expressed higher programmed cell death protein 1 (PD-1) and inducible costimulatory (ICOS) in AChR-MG and SN-MG groups, mostly irrespective of the treatment. Based on chemokine receptors on CXCR5(+)PD-1(+) in CD4(+) T (cTfh) cells, in AChR-MG patients without treatment, the proportions of Tfh17 cells were higher than those in the treated group, whereas the Tfh1 cells were decreased compared with those in the controls. The relevance of CXCR5 and PD-1 in the pathogenesis of AChR-MG was also suggested by the increased presence of these molecules on mature CD4 single-positive thymocytes from the thymic samples. The study provides further evidence for the importance of IL-21, IL-17A, IL-4, and IL-10 in AChR-MG. Disease-related CD4(+)T cells are identified mainly as PD-1(+) or ICOS+ with or without CXCR5, resembling cTfh cells in the circulation or probably in the thymus. AChR-MG and SN-MG seem to have some similar characteristics. IS treatment has distinctive effects on cytokine expression.Istanbul Universit

The Effect of Fucoidan on Prostate Cancer Cells

The Effect of Fucoidanon Prostate Cancer Cells</span

The Effects of SAP3 and SAP6 Molecules on DU-145 Cells; PreliminaryStudy.

The Effects of SAP3 andSAP6 Molecules on DU-145 Cells; PreliminaryStudy.</p