Inventaire des plantes-hôtes d'Aphis gossypii Glover (Hemiptera, Aphididae) en Afrique

L'inventaire des plantes-hôtes d'#Aphis gossypii$ sur le continent africain est établi à partir de données bibliographiques en tenant compte des dernières modifications et synonymies de la classification botanique. 658 espèces appartenant à 103 familles et 420 genres sont recensées. (Résumé d'auteur

Long term human primary hepatocyte cultures in a microfluidic liver biochip show maintenance of mRNA levels and higher drugs metabolisms when compared to Petri cultures.

International audienceHuman primary hepatocytes were cultivated in a microfluidic bioreactor and in Petri dishes for 13 days. mRNA kinetics in biochips showed an increase in the levels of CYP2B6, CYP2C19, CYP2C8, CYP3A4, CYP1A2, CYP2D6, HNF4a, SULT1A1, UGT1A1 mRNA related genes when compared with post extraction levels. In addition, comparison with Petri dishes showed higher levels of CYP2B6, CYP2C19, CYP2C8, CYP3A4, CYP1A2, CYP2D6 related genes at the end of culture. Functional assays illustrated a higher urea and albumin production over the period of culture in biochips. Bioreactor drug metabolism (midazolam and phenacetin) was not superior to the Petri dish after 2 days of culture. The CYP3A4 midazolam metabolism was maintained in biochips after 13 days of culture, whereas it was almost undetectable in Petri dishes. This led to a 5000-fold higher value of the metabolic ratio in the biochips. CYP1A2 phenacetin metabolism was found to be higher in biochips after 5, 9 and 13 days of culture. Thus, a 100-fold higher metabolic ratio of APAP in biochips was measured after 13 days of perfusion. These results demonstrated functional primary human hepatocyte culture in the bioreactor in a long-term culture

Grape seeds valorization: extraction of polyphenols by electrical treatments and ultrasound

19th International Congress of Chemical and Process Engineering - CHISA 2010 & 7th European Congress of Chemical Engineering - ECCE-7, Prague, Czech Republic, 28 August - 1 September 2010

Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.

International audienceBetanin is a natural pigment with significant antioxidant and biological activities currently used as food colorant. The isolation of betanin is problematic due to its instability. In this work, we developed a fast and economic procedure based on molecularly imprinted solid-phase extraction (MISPE) for the selective clean-up of betanin and its stereoisomer isobetanin from beetroot extracts. Dipicolinic acid was used as template for the MIP preparation because of its structural similarity with the chromophore group of betanin. The MISPE procedures were fully optimized allowing the almost complete removal of matrix components such as sugars and proteins, resulting in high extraction recovery of betanin/isobetanin in a single step. Moreover, the whole extraction procedure was performed in environmentally friendly solvents with either ethanol or water. Our MISPE method is very promising for the future development of well-formulated beetroot extract with specified betanin/isobetanin content, ready for food or medicinal use

2R-regeneration in a monolithically integrated four-section SOA-EA chip

Optical regeneration using a monolithically integrated chip formed by a cascade of semiconductor optical amplifiers and saturable absorbers is investigated. Static transfer functions, signal reshaping, extinction ratio enhancement, noise dynamics and device dependence on operation conditions are measured. Results show that by cascading two-pairs of SOA–EAs a steep static transfer function is achieved. Dynamical measurements show large improvements in extinction ratio as well as a large improvement in the receiver-sensitivity when used as a regenerator for NRZ signals at 10 Gb/s