1 research outputs found
Transcriptome and Metabolome Analysis Reveals the Effect of Nitrogen–Potassium on Anthocyanin Biosynthesis in “Fuji” Apple
Nitrogen
(N) and potassium (K) have significant effects on apple
peel color. To further understand the molecular mechanism of N–K
regulation of apple color, we analyzed the apple peel under different
N and K treatments using isotope labeling, transcriptomics, and metabolomics.
Under high N treatments, fruit red color and anthocyanin content decreased
significantly. High N decreased the 13C distribution rate
and increased the Ndff values of fruits, while K increased the expression
of MdSUTs and MdSOTs and promoted 13C transportation to fruits. Anthocyanin-targeted metabonomics
and transcriptome analysis revealed that high N downregulated the
expression of structural genes related to the anthocyanin synthesis
pathway (MdPAL, Md4CL, MdF3H, MdANS, and MdUFGT) and their
regulators (MdMYBs and MdbHLHs),
and also decreased some metabolites contents. K alleviated this inhibition
and seven anthocyanins were regulated by N–K. Our results improve
the understanding of the synergistic regulation of apple fruit coloring
by N–K