Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization.

Soil types heavily influence ecological dynamics. It remains controversial to what extent soil types shape microbial responses to land management changes, largely due to lack of in-depth comparison across various soil types. Here, we collected samples from three major zonal soil types spanning from cold temperate to subtropical climate zones. We examined bacterial and fungal community structures, as well as microbial functional genes. Different soil types had distinct microbial biomass levels and community compositions. Five years of maize cropping (growing corn or maize) changed the bacterial community composition of the Ultisol soil type and the fungal composition of the Mollisol soil type but had little effect on the microbial composition of the Inceptisol soil type. Meanwhile, 5 years of fertilization resulted in soil acidification. Microbial compositions of the Mollisol and Ultisol, but not the Inceptisol, were changed and correlated (P < 0.05) with soil pH. These results demonstrated the critical role of soil type in determining microbial responses to land management changes. We also found that soil nitrification potentials correlated with the total abundance of nitrifiers and that soil heterotrophic respiration correlated with the total abundance of carbon degradation genes, suggesting that changes in microbial community structure had altered ecosystem processes. IMPORTANCE Microbial communities are essential drivers of soil functional processes such as nitrification and heterotrophic respiration. Although there is initial evidence revealing the importance of soil type in shaping microbial communities, there has been no in-depth, comprehensive survey to robustly establish it as a major determinant of microbial community composition, functional gene structure, or ecosystem functioning. We examined bacterial and fungal community structures using Illumina sequencing, microbial functional genes using GeoChip, microbial biomass using phospholipid fatty acid analysis, as well as functional processes of soil nitrification potential and CO2 efflux. We demonstrated the critical role of soil type in determining microbial responses to land use changes at the continental level. Our findings underscore the inherent difficulty in generalizing ecosystem responses across landscapes and suggest that assessments of community feedback must take soil types into consideration. Author Video: An author video summary of this article is available

Stochastic evolutionary game analysis of food cooperation among countries along the Belt and Road from the perspective of food security

IntroductionIn recent years, the combination of the public health crisis and the climate crisis has greatly weakened the resilience of food systems. The Belt and Road food cooperation will make outstanding contributions to consolidate food security and reduce global poverty in countries along the Belt and Road.MethodsThis paper constructs a three-party stochastic evolutionary game model from the perspective of food security, distinguishes the difference between large and small countries by calculating the cereal self-sufficiency rate, which studies the cooperation strategy with the Belt and Road cereal large countries, cereal small countries and regulatory committees as the object. In addition, we introduce the Gaussian white noise characteristics to characterize the stochastic disturbance of the game system. Then we combine the mathematical derivation of the stochastic evolutionary game analysis with the simulation method, examine the effect of the stochastic disturbance factors on the cooperation, and find out the factors that affect the cooperation. And finally verifies the model and proves the reasonableness and practicability of the model.ResultsThe stochastic evolutionary game model analyzes the interaction of stochastic disturbance and influencing factors from a dynamic perspective, and the stability of evolution is related to the strength of Gaussian white noise. The level of effort of large cereal countries and the incentives of regulatory committees can have a positive impact, but high income in small cereal countries can lead to instability in the strategic choices of other players.DiscussionTaking the countries along the Belt and Road as the research object, Gaussian white noise is introduced to describe the stochastic external environment, discriminate the stability of the game system through stochastic differential equations, and analyze the influencing factors of the dynamic behavioral strategies of the parties in combination with simulation methods. To improve the capacity of national food security governance and help improve the resilience of the world food system

Antibody-Targeted Immunocarriers for Cancer Treatment

Nanocarrier’s engineering based on fine chemical design and novel structural tailoring can provide practical solution to solve the problems in traditional cancer immunotherapy. Nanoimmunotherapy is thus defined as the application and further development of novel nanocarriers for enhancing immunotherapy. It has become one of the most intriguing fields due to its unique power in treatment and even cure of cancer since reported in last year. Herein, this chapter illustrates the state-of-the-art development in antibody engineering and cancer immunotherapy and gives an explanation why functional nanocarries including micelles and liposomes can be efficient for nanoimmunotherapy. We further illustrate how to promote the nanoimmunotherapy by the chemical design and carrier’s engineering for the first time

Research on a symmetric non-resonant piezoelectric linear motor

Nowadays, piezoelectric linear actuators draw wide attention of researchers around world as its advantages of simple structure, high precision and rapid response. To improve the performance of the non-resonant piezoelectric motor, a symmetric piezoelectric linear motor driven by double-foot is studied in the paper. The vibration model of the stator is established based on the structure and the working mechanism of motor. Then guide mechanism and preload device is designed and a prototype is fabricated to verify the feasibility of structure. The performances of motor under different driving signal are tested in experiment. By applying three-phase square-triangular waves signal and four-phase sine waves signal of peak to peak value 100 V with 50 V offset and frequency of 100 Hz, the speed of prototype reaches 733 μm/s and 667 μm/s and the maximum thrust is 8.34 N and 6.31 N respectively

Microbial responses to inorganic nutrient amendment overridden by warming: Consequences on soil carbon stability.

Eutrophication and climate warming, induced by anthropogenic activities, are simultaneously occurring worldwide and jointly affecting soil carbon stability. Therefore, it is of great interest to examine whether and how they interactively affect soil microbial community, a major soil carbon driver. Here, we showed that climate warming, simulated by southward transferring Mollisol soil in agricultural ecosystems from the cold temperate climate zone (N) to warm temperate climate (C) and subtropical climate zone (S), decreased soil organic matter (SOM) by 6%-12%. In contrast, amendment with nitrogen, phosphorus and potassium enhanced plant biomass by 97% and SOM by 6% at the N site, thus stimulating copiotrophic taxa but reducing oligotrophic taxa in relative abundance. However, microbial responses to nutrient amendment were overridden by soil transfer in that nutrient amendment had little effect at the C site but increased recalcitrant carbon-degrading fungal Agaricomycetes and Microbotryomycetes taxa derived from Basidiomycota by 4-17 folds and recalcitrant carbon-degrading genes by 23%-40% at the S site, implying a possible priming effect. Consequently, SOM at the S site was not increased by nutrient amendment despite increased plant biomass by 108%. Collectively, we demonstrate that soil transfer to warmer regions overrides microbial responses to nutrient amendment and weakens soil carbon sequestration

Dietary patterns and breast cancer risk, prognosis, and quality of life: A systematic review

BackgroundStatistics indicate that the morbidity of breast cancer is increasing globally, and its (overall figures) incidence has now surpassed that of lung cancer for the first time. The relation between a whole dietary pattern, rather than of a single food or nutrient, and breast cancer (BC) should be examined for findings to capture the complexities of diet and the potential for synergism between dietary components. Hence, the effects of dietary patterns on breast cancer have recently attracted increasing attention.ObjectiveTo systematically review the effects of dietary patterns on breast cancer risk, prognosis, and quality of life in survivors.MethodsThis systematic review was conducted following PRISMA guidelines and was registered in PROSPERO. Data from Ovid, China Biomedical Literature Database, Wanfang Data Knowledge Service Platform, CNKI, PubMed, Weipu, The Cochrane Library, Duxiu Data, ProQuest, Embase, Web of Science, and Scopus Database were retrieved and evaluated.ResultsA total of 47 studies that investigated the association between eating patterns and breast cancer were identified. Ten studies evaluated the effect of the model on treatment outcome and prognosis of breast cancer and two cross-sectional studies examined the influence of dietary patterns on quality of life. The resulting favorable dietary patterns were shown to regulate metabolic biomarkers, antioxidants, anti-inflammatory agents, and protective genes, and inhibit cell proliferation and invasion.ConclusionNumerous studies have examined the effects of healthy eating, plant-based, anti-inflammation, low-fat, and other favorable dietary patterns in relation to breast cancer. However, few studies reported significant associations and the studies had limitations, suggesting that the current findings should be interpreted with caution.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, CRD4202 2350171

Design and Experiment of a Clamping-Drive Alternating Operation Piezoelectric Actuator

In recent years, piezoelectric actuators, represented by inertial and inchworm actuators, have been widely applied because of their high accuracy and excellent responsiveness. Despite the development of various piezoelectric actuators, there remain some flaws in this technology. The sticking point is that the piezoelectric actuators based on the friction driving principle are prone to unwanted backward motion when outputting stepping motion. It is thus urgent to explore solutions from the perspectives of principle and structure. In this paper, a clamping-drive alternating operation piezoelectric actuator is proposed, the two feet of which are driven by two piezoelectric stacks, respectively. Due to double-foot alternate drive guide movement, backward movement is prevented in theory. By adopting the double-layer stator structure, integrated processing and assembly are facilitated. Meanwhile, a double flexible hinge mechanism is installed in the stator to prevent the drive foot from being overturned due to ineffectiveness and premature wear. In addition, the stator is equipped with the corresponding preload mechanism and clamping device. After the cycle action mechanism of one cycle and four steps is expounded, a model is established in this study to further demonstrate the principle. With the prototype produced, a series of experiments are performed. In addition, the amplitude of actuation of the stator is tested through amplitude experiment. The performance of the stator is evaluated by conducting experiments in the alternating step and single step actuation modes. Finally, the test results are analyzed to conclude that the actuator operating in either of these two modes can meet the practical needs of macro and micro actuation

Design, Analysis, and Experiment on a Novel Stick-Slip Piezoelectric Actuator with a Lever Mechanism

A piezoelectric actuator using a lever mechanism is designed, fabricated, and tested with the aim of accomplishing long-travel precision linear driving based on the stick-slip principle. The proposed actuator mainly consists of a stator, an adjustment mechanism, a preload mechanism, a base, and a linear guide. The stator design, comprising a piezoelectric stack and a lever mechanism with a long hinge used to increase the displacement of the driving foot, is described. A simplified model of the stator is created. Its design parameters are determined by an analytical model and confirmed using the finite element method. In a series of experiments, a laser displacement sensor is employed to measure the displacement responses of the actuator under the application of different driving signals. The experiment results demonstrate that the velocity of the actuator rises from 0.05 mm/s to 1.8 mm/s with the frequency increasing from 30 Hz to 150 Hz and the voltage increasing from 30 V to 150 V. It is shown that the minimum step distance of the actuator is 0.875 μm. The proposed actuator features large stroke, a simple structure, fast response, and high resolution