2 research outputs found
Solvatochromic Two-Photon Fluorescent Probe Enables <i>In Situ</i> Lipid Droplet Multidynamics Tracking for Nonalcoholic Fatty Liver and Inflammation Diagnoses
Intracellular
lipid storage and regulation occur in lipid
droplets,
which are of great significance to the physiological activities of
cells. Herein, a lipid droplet-specific fluorescence probe (lip-YB) with a high quantum yield (QYlip‑YB = 73.28%), excellent photostability, and quickly polarity
sensitivity was constructed successfully. Interestingly, lip-YB exhibited remarkable two-photon (TP) characteristics, which first
realized real-time monitoring of the lipid droplet multidynamics process,
diagnosing nonalcoholic fatty liver disease (NAFLD) and inflammation
in living mice via TP fluorescence imaging. It is found that the as-prepared lip-YB provides a new avenue to design lipid droplet-specific
imaging probes, clarifies its roles and mechanisms in cell metabolism,
and can timely intervene in lipid droplet-related diseases during
various physiological and pathological processes
Auxochrome Dimethyl-Dihydroacridine Improves Fluorophores for Prolonged Live-Cell Super-Resolution Imaging
Superior
photostability, minimal phototoxicity, red-shifted absorption/emission
wavelengths, high brightness, and an enlarged Stokes shift are essential
characteristics of top-tier organic fluorophores, particularly for
long-lasting super-resolution imaging in live cells (e.g., via stimulated
emission depletion (STED) nanoscopy). However, few existing fluorophores
possess all of these properties. In this study, we demonstrate a general
approach for simultaneously enhancing these parameters through the
introduction of 9,9-dimethyl-9,10-dihydroacridine (DMA) as an electron-donating
auxochrome. DMA not only induces red shifts in emission wavelengths
but also suppresses photooxidative reactions and prevents the formation
of triplet states in DMA-based fluorophores, greatly improving photostability
and remarkably minimizing phototoxicity. Moreover, the DMA group enhances
the fluorophores’ brightness and enlarges the Stokes shift.
Importantly, the “universal” benefits of attaching the
DMA auxochrome have been exemplified in various fluorophores including
rhodamines, difluoride-boron complexes, and coumarin derivatives.
The resulting fluorophores successfully enabled the STED imaging of
organelles and HaloTag-labeled membrane proteins