Effect of Huatan Quyu Decoction on Patients with Cerebral Infarction

The objective is to explore the clinical methods and effects of applying Huatan Quyu Decoction in the treatment of patients with cerebral infarction. The research work was carried out in our hospital, and the research was carried out from November 2019 to November 2020. Patients with cerebral infarction were selected as the research object, and the number of patients was 80. They were randomly divided into two groups and one group was given conventional rehabilitation therapy as the control group, the other group was given Huatan Quyu Decoction as the experimental group, and the effects of different treatments on patients were compared and analyzed. Before treatment intervention, there was no significant difference in the scores of neurological impairment, limb motor ability and ability of daily living between the two groups of patients, P>0.05. After treatment intervention, the CSS score of the experimental group decreased and the BI score increased, which has significant differences compared with the control group, and the experimental group has higher scores for sensory and motor function. In addition, the effective rate of treatment in the experimental group was 95.00%, which was significantly higher than the 77.50% in the control group. The difference between the two groups was significant and meaningful. The treatment effect was better in the experimental group.The application of Huatan Quyu Decoction in the process of treating patients with cerebral infarction can effectively promote the recovery of patients and improve the quality of life of patients. The clinical application is significant

Refugial isolation and range expansions drive the genetic structure of \u3cem\u3eOxyria sinensis\u3c/em\u3e (Polygonaceae) in the Himalaya-Hengduan Mountains

The formation of the Mekong-Salween Divide and climatic oscillations in Pleistocene were the main drivers for the contemporary diversity and genetic structure of plants in the Himalaya-Hengduan Mountains (HHM). To identify the relative roles of the two historical events in shaping population history of plants in HHM, we investigated the phylogeographic pattern of Oxyria sinensis, a perennial plant endemic to the HHM. Sixteen chloroplast haplotypes were identified and were clustered into three phylogenetic clades. The age of the major clades was estimated to be in the Pleistocene, falling into several Pleistocene glacial stages and postdating the formation of the Mekong-Salween Divide. Range expansions occurred at least twice in the early and middle Pleistocene, but the spatial genetic distribution rarely changed since the Last Glacial Maximum. Our results suggest that temporary mountain glaciers may act as barriers in promoting the lineage divergence in O. sinensis and that subsequential range expansions and secondary contacts might reshape the genetic distribution in geography and blur the boundary of population differentiation created in the earlier glacial stages. This study demonstrates that Pleistocene climatic change and mountain glaciers, rather than the Mekong-Salween Divide, play the primary role in shaping the spatial genetic structure of O. sinensis

Antagonism of miR-21 Reverses Epithelial-Mesenchymal Transition and Cancer Stem Cell Phenotype through AKT/ERK1/2 Inactivation by Targeting PTEN

BACKGROUND: Accumulating evidence suggested that epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) characteristics, both of which contribute to tumor invasion and metastasis, are interrelated with miR-21. MiR-21 is one of the important microRNAs associated with tumor progression and metastasis, but the molecular mechanisms underlying EMT and CSC phenotype during miR-21 contributes to migration and invasion of breast cancer cells remain to be elucidated. METHODOLOGY/PRINCIPAL FINDINGS: In this study, MDA-MB-231/anti-miR-21 cells were established by transfected hsa-miR-21 antagomir into breast cancer MDA-MB-231 cells. EMT was evaluated by the changes of mesenchymal cell markers (N-cadherin, Vimentin, and alpha-SMA), epithelial cell marker (E-cadherin), as well as capacities of cell migration and invasion; CSC phenotype was measured using the changes of CSC surface markers (ALDH1 and CD44), and the capacity of sphereforming (mammospheres). We found that antagonism of miR-21 reversed EMT and CSC phenotype, accompanied with PTEN up-regulation and AKT/ERK1/2 inactivation. Interestingly, down-regulation of PTEN by siPTEN suppressed the effects of miR-21 antagomir on EMT and CSC phenotype, confirming that PTEN is a target of miR-21 in reversing EMT and CSC phenotype. The inhibitors of PI3K-AKT and ERK1/2 pathways, LY294002 and U0126, both significantly suppressed EMT and CSC phenotype, indicating that AKT and ERK1/2 pathways are required for miR-21 mediating EMT and CSC phenotype. CONCLUSIONS/SIGNIFICANCE: In conclusion, our results demonstrated that antagonism of miR-21 reverses EMT and CSC phenotype through targeting PTEN, via inactivation of AKT and ERK1/2 pathways, and showed a novel mechanism of which might relieve the malignant biological behaviors of breast cancer

Effect of Huatan Quyu Decoction on Patients with Cerebral Infarction

The objective is to explore the clinical methods and effects of applying Huatan Quyu Decoction in the treatment of patients with cerebral infarction. The research work was carried out in our hospital, and the research was carried out from November 2019 to November 2020. Patients with cerebral infarction were selected as the research object, and the number of patients was 80. They were randomly divided into two groups and one group was given conventional rehabilitation therapy as the control group, the other group was given Huatan Quyu Decoction as the experimental group, and the effects of different treatments on patients were compared and analyzed. Before treatment intervention, there was no significant difference in the scores of neurological impairment, limb motor ability and ability of daily living between the two groups of patients, P>0.05. After treatment intervention, the CSS score of the experimental group decreased and the BI score increased, which has significant differences compared with the control group, and the experimental group has higher scores for sensory and motor function. In addition, the effective rate of treatment in the experimental group was 95.00%, which was significantly higher than the 77.50% in the control group. The difference between the two groups was significant and meaningful. The treatment effect was better in the experimental group.The application of Huatan Quyu Decoction in the process of treating patients with cerebral infarction can effectively promote the recovery of patients and improve the quality of life of patients. The clinical application is significant

Paolo Tripodi: Dimenticare Wittgenstein. Una vicenda della filosofia analitica, Bologna, Il Mulino, 2009.

Paolo Tripodi ripercorre la vicenda della ricezione del pensiero di Wittgenstein nella filosofia analitica, soffermandosi in particolare sulle ragioni che l'hanno indotta a "dimenticare Wittgenstein"

Comparative analysis of genome sequences of the two cultivated tetraploid cottons, Gossypium hirsutum (L.) and G. barbadense (L.)

With innovations in sequencing technology and the progress of high-performance computing systems, it is now relatively straightforward to sequence and assemble complex genomes. Many genomes from multiple cotton species have been released in recent years, with the highly homozygous standard genetic lines of two cultivated allotetraploid cottons, i.e., Gossypium hirsutum TM-1 and G. barbadense 3–79, assembled multiple times by different research groups using diverse sequencing technologies. The assembly quality among these genomes is variable, even between multiple accessions or versions of the same species, which can generate both confusion in choosing the appropriate genome for genetic analysis and obstacles when comparing results among the different reference genomes. Accordingly, an assessment of the many cotton genome sequences is necessary to facilitate both choice of genome sequence and comparisons between different versions or species. Here we comprehensively assess and compare genome assembly accuracy, completeness, and contiguity for nine G. hirsutum assemblies and four G. barbadense assemblies using multiple analysis strategies with the same criteria. We identify centromeric regions and several large-scale inversions among genomes from the same accession, indicating structural errors introduced during sequence ordering and orientation in G. hirsutum and G. barbadense genome assembly. Gene relationships between annotations from multiple genomes are defined within and across species, and the results are available at the Cotton Paralogs Groups Search website (https://ihope.shinyapps.io/cottonParalogs/), a convenient resource for converting gene ids and comparing annotations between different genome versions. This study comprehensively assesses and compares assembly quality among multiple versions of the two cultivated tetraploid cotton species with different assembly strategies, illustrating the challenges of sequencing and assembling complex genomes and providing a resource for cotton genomics.This article is published as Meng, Qingying, Jiaqi Gu, Zhongping Xu, Jie Zhang, Jiwei Tang, Anzhou Wang, Ping Wang et al. "Comparative analysis of genome sequences of the two cultivated tetraploid cottons, Gossypium hirsutum (L.) and G. barbadense (L.)." Industrial Crops and Products 196 (2023): 116471. doi:https://doi.org/10.1016/j.indcrop.2023.116471.Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted