Natural IgM production in PEC.

<p>PEC lavage before and after <i>C. albicans</i> inoculation were detected by ELISA using plate coated with anti-mouse IgM antibody or live <i>C. albicans</i>. (A) Total IgM, (B) <i>C.albicans</i>-specific IgM. Data shown are means±SD, n = 6. <b>*</b><i>P</i><0.01.</p

g‑C₃N₄/Metal–Organic Framework Nanosheet/CuO Heterostructure for the Visible Photocatalytic Degradation of Tetracycline

Developing highly efficient photocatalysts for the degradation of pharmaceutical and personal care product residues is of significance and challenge. Herein, a graphitic carbon nitride (g-C3N4)/metal–organic framework (MOF) nanosheet/CuO heterostructure was successfully constructed via electrostatic self-assembly and in situ growth strategies. The heterostructure at ultralow doses (6 mg) displayed the highest tetracycline (TC, 20 mg/L) degradation efficiency of 78.8% under visible light within 27 min. Moreover, this heterostructure maintained a good TC degradation efficiency after four cycles. Experimental data and density functional theory calculations demonstrate that the good photocatalytic performance of the as-prepared heterostructure is attributed to the positively charged MOF sheet interlayer and the coupling effect between the g-C3N4, MOF nanosheet and CuO, which can enrich electrons, ions, molecules, and block holes to greatly improve the rapid separation of photogenerated carriers from g-C3N4 or CuO and the adsorption of reactants. The types of reactive oxygen species and effects of inorganic ions on photocatalytic performance were further studied. This work presented a novel strategy to construct a self-assembled heterostructure for visible photocatalytic applications

<i>C. albicans</i> burden and inflammatory infiltration after i. p. inoculation.

<p>2×10⁶<i>C. albicans</i> yeasts were i. p. injected into TgV_H3B4 mice and littermate. At different time point the mice were sacrificed and PEC lavage was eluted by PBS. Results obtained from elution at 36 h after the inoculation were shown. Living yeasts in the elution were assessed by a colony-forming assay (A), and supernatant of the elution was subjected to inflammation cytokines analysis by a Cytometry Beads Array kit (B). Data shown are means±SD, n = 6. <b>*</b><i>P</i><0.01. (C) Cells collected from the PEC elution were stained with antibodies and analyzed by FCM. Data are representative of four independent experiments.</p

BrdU incorporation assay.

<p>BrdU was i. p. injected with <i>C. albicans</i> inoculation to TgV_H3B4 mice and littermate, and injected for the second time 12 h later. PEC cells were eluted at 36 h after inoculation and BrdU labeling was performed using a BrdU Labeling kit and analyzed by FCM. Data are representative of four independent experiments.</p

B cell analysis in PEC.

<p>Cells collected the PEC lavage before and after <i>C.albicans</i> inoculation were stained with antibodies and analyzed by FCM. Data are representative of four independent experiments.</p

Building Sustainable Saturated Fatty Acid-Zinc Interfacial Layer toward Ultra-Stable Zinc Metal Anodes

The commercialization pace of aqueous zinc batteries (AZBs) is seriously limited due to the uncontrolled dendrite growth and severe corrosion reaction of the zinc anode. Herein, a universal and extendable saturated fatty acid-zinc interfacial layer strategy for modulating the interfacial redox process of zinc toward ultrastable Zn metal anodes is proposed. The in situ complexing of saturated fatty acid-zinc interphases could construct an extremely thin zinc compound layer with continuously constructed zincophilic sites which kinetically regulates Zn nucleation and deposition behaviors. Furthermore, the multifunctional interfacial layer with internal hydrophobic carbon chains as a protective layer is efficient to exclude active water molecules from the surface and efficiently inhibit the surface corrosion of zinc. Consequently, the modified anode shows a long cycle life of over 4000 h at 5 mA cm–2. In addition, the assembled Zn||V2O5 full cells based on modified zinc anodes have excellent rate performance and long cycle stability

Comparison of sFasL release between PBMCs and cultured T cells.

<p>PBMCs from patient 1 and 5 were cultured with causal drugs for 10 days. The cells were subsequently washed, and re-stimulated with causal drugs for 3 days. The sFasL levels in supernatants were determined by sFasL ELISA kit and were compared with those in 3-day PBMC culture supernatants. Results represent mean ± SD from three independent experiments. *<i>P<0.05</i>.</p

No change of sFasL level in supernatants of PBMCs upon stimulation with higher concentration of amoxicillin.

<p>PBMCs from patient 3 and patient 7 were cultured with amoxicillin at concentrations of 0, 40, 80,160 μg/ml. The sFasl levels were then determined by ELISA. A. Patient 3. B, Paitent 7. Results represent mean ± SD from three independent experiments. *<i>P<0.05</i>.</p

<i>In vitro</i> toxicities of PBMC supernatant against keratinocytes.

<p>HaCat cells were incubated with culture medium alone or culture medium containing 5%, 10%, 20% PBMC supernatants for 24 hours. A. MTT assays showed that the supernatants of drug-stimulated PBMCs were cytotoxic against keratinocytes in a dose-dependent manner. B. Annexin V staining showed that the supernatants of drug-stimulated PBMCs induced apoptosis of keratinocytes in a dose – dependent manner. *<i>P<0.05</i>.</p

sFasL mAb blocking decreased the percentage of apoptotic keratinocytes. HaCat cells were incubated with culture medium containing 10% PBMC supernatants, the neutralizing anti-FasL mAb (NOK-2), the negative control mAb for 24 hours.

<p>Annexin V staining showed that the inhibitory anti-FasL mAb reduced the number of apoptotic cells in a dose-dependent manner. *<i>P<0.05</i>.</p