The Effects of Cisplatin on MTOR, AKT, CCND1 and STAT3 mRNA Expressions on HeLa Cells

WOS: 000505071500002Objective: Cervical cancer is one of the most tumors seen in womans and second leading cause of cancer death in women. Cisplatin (CDDP), a platin based compound, is the most important chemotherapeutic agent and effectively used for the treatment of sarcomas and solid tumors. It binds to the DNA with crosslinks and leads inhibition of replication, causing DNA damage. As a result of this action, apoptotic pathways are induced and cell death occurs. In our study, we aimed to investigate the effect of CDDP on relative mRNA expression of mTOR, AKT, Cyclin D1 (CCND1) ve STAT3 on cervical cancer cell line. Method: In this study, HeLa cells were treated with different concentrations of CDDP at 24 and 48 hours. Cell viability was determined by XTT method. Moreover, after treatment with selected doses of cisplatin, quantitative mRNA expression of mTOR, AKT, CCND1 and STAT-3 genes was analyzed using Real-Time PCR. Results: IC50 concentration of CDDP was found to be about 60 mu M for 24h and 8 mu M for 48h treatment. Moreover, all analyzed genes' expression was shown to diminish only after 24 h treatment. On the other hand, no statistically significant change was found after 48 h cisplatin exposure with respect to quantitative mRNA expression. Conclusion: In summary, different mRNA expression pattern was found after CDDP treatment regarding to exposure time. Our study has been contributed the literature in terms of detecting the effect of conventional chemotherapeutic CDDP on cell survival pathways

Association between TNF-beta NcoI (A252G) gene polymorphism and type I diabetes mellitus in Turkish population

WOS: 000314288600004Objective: Diabetes mellitus is a common metabolic disorder characterized by hyperglycemia. Cytokines induce signal transduction and secondary messenger pathways after binding their specific ligands. In this way, these proteins control and also modulate activity of the target cells. Tumor necrosis factor plays an important role in diabetes and is a member of the family of cytokines. In this study, we aimed to investigate the effect of A252G (TNF-beta NcoI, rs909253) polymorphism in the tumor necrosis factor beta gene on the Type I Diabetes Mellitus in the Turkish population. Materials and Method: Distribution of A252G polymorphism was determined in 96 patients with Type I diabetes and 101 healty individuals by PCR-RFLP. Results: When obtained data was evaluated, the A allele of A252G polymorphism were observed more frequently than G allele in patients and controls. Moreover, G alleles were observed more frequently among patients than in controls. This high frequency of G allele found in patients demonstrates a 1.6 fold increased risk of susceptibility to type I diabetes in Turkish population (P=0.032). However, there was no significant difference between patients and controls in terms of the distribution of genotypes (P=0.132). Conclusion: As a result, although presence of a G allele demonstrated an increased risk of susceptibilty to type I diabetes, TNF-beta A252G polymorphism's genotype distrubution was not associated with type I diabetes in Turkish population. Polymorphisms involved in the development of Type I Diabetes Mellitus results in better understanding of the development and the progression of disease