{HST Studies of the WLM Galaxy. II. The Star Formation History from Field Stars

HST F555W and F814W photometry of a portion of the WLM galaxy are presented. The distance modulus is determined via fitting of the entire color-magnitude diagram to be (m-M)_0 = 24.88 +/- 0.09, which is consistent with the RGB tip distance. The galaxy's measurable star formation history appears to have begun no more than 12 Gyr ago, with about half of WLM's total star formation (by mass) formed before 9 Gyr ago. The star formation rate gradually decreased, until a recent increase in activity starting between 1 and 2.5 Gyr ago. This is still continuing to the present time, and is concentrated in the bar of the galaxy, as shown by the difference in recent star formation rates in the three WF chips.Comment: 20 pages, 7 figures to be published in Ap

Turbulent superfluid profiles in a counterflow channel

We have developed a two-dimensional model of quantised vortices in helium II moving under the influence of applied normal fluid and superfluid in a counterflow channel. We predict superfluid and vortex-line density profiles which could be experimentally tested using recently developed visualization techniques.Comment: 3 double figures, 9 page

Flow Phase Diagram for the Helium Superfluids

The flow phase diagram for He II and $^3$He-B is established and discussed based on available experimental data and the theory of Volovik [JETP Letters {\bf{78}} (2003) 553]. The effective temperature - dependent but scale - independent Reynolds number $Re_{eff}=1/q=(1+\alpha')/\alpha$, where $\alpha$ and $\alpha'$ are the mutual friction parameters and the superfluid Reynolds number characterizing the circulation of the superfluid component in units of the circulation quantum are used as the dynamic parameters. In particular, the flow diagram allows identification of experimentally observed turbulent states I and II in counterflowing He II with the turbulent regimes suggested by Volovik.Comment: 2 figure

Investigating The Possible Anomaly Between Nebular and Stellar Oxygen Abundances in the Dwarf Irregular Galaxy WLM

We obtained new optical spectra of 13 H II regions in WLM with EFOSC2; oxygen abundances are derived for nine H II regions. The temperature-sensitive [O III] 4363 emission line was measured in two bright H II regions HM7 and HM9. The direct oxygen abundances for HM7 and HM9 are 12+log(O/H) = 7.72 +/- 0.04 and 7.91 +/- 0.04, respectively. We adopt a mean oxygen abundance of 12+log(O/H) = 7.83 +/- 0.06. This corresponds to [O/H] = -0.83 dex, or 15% of the solar value. In H II regions where [O III] 4363 was not measured, oxygen abundances derived with bright-line methods are in general agreement with direct values of the oxygen abundance to an accuracy of about 0.2 dex. In general, the present measurements show that the H II region oxygen abundances agree with previous values in the literature. The nebular oxygen abundances are marginally consistent with the mean stellar magnesium abundance ([Mg/H] = -0.62). However, there is still a 0.62 dex discrepancy in oxygen abundance between the nebular result and the A-type supergiant star WLM15 ([O/H] = -0.21). Non-zero reddening values derived from Balmer line ratios were found in H II regions near a second H I peak. There may be a connection between the location of the second H I peak, regions of higher extinction, and the position of WLM15 on the eastern side of the galaxy.Comment: Accepted, Ap.J.; 19 pages (AASTeX 5.2) with 6 figures. Full paper with color figures at http://www.astro.umn.edu/~hlee

What next for preimplantation genetic screening? High mitotic chromosome instability rate provides the biological basis for the low success rate

Preimplantation genetic screening is being scrutinized, as recent randomized clinical trials failed to observe the expected significant increase in live birth rates following fluorescence in situ hybridization (FISH)-based screening. Although these randomized clinical trials are criticized on their design, skills or premature stop, it is generally believed that well-designed and well-executed randomized clinical trials would resolve the debate about the potential benefit of preimplantation genetic screening. Since FISH can analyze only a limited number of chromosomal loci, some of the embryos transferred might be diagnosed as ‘normal’ but in fact be aneuploid for one or more chromosomes not tested. Hence, genome-wide array comparative genome hybridization screening enabling aneuploidy detection of all chromosomes was thought to be a first step toward a better design. We recently showed array screening indeed enables accurate determination of the copy number state of all chromosomes in a single cell. Surprisingly, however, this genome-wide array screening revealed a much higher frequency and complexity of chromosomal aberrations in early embryos than anticipated, with imbalances in a staggering 90% of all embryos. The mitotic error rate in cleavage stage embryos was proven to be higher than the meiotic aneuploidy rate and as a consequence, the genome of a single blastomere is not representative for the genome of the other cells of the embryo. Hence, potentially viable embryos will be discarded upon screening a single blastomere. This observation provides a biological basis for the failure of the randomized clinical trials to increase baby-take-home rates using FISH on cleavage stage embroys

A Spitzer/IRAC Census of the Asymptotic Giant Branch Populations in Local Group Dwarfs. I. WLM

We present Spitzer/IRAC observations at 3.6 and 4.5 microns along with optical data from the Local Group Galaxies Survey to investigate the evolved stellar population of the Local Group dwarf irregular galaxy WLM. These observations provide a nearly complete census of the asymptotic giant branch (AGB) stars. We find 39% of the infrared-detected AGB stars are not detected in the optical data, even though our 50% completeness limit is three magnitudes fainter than the red giant branch tip. An additional 4% of the infrared-detected AGBs are misidentified in the optical, presumably due to reddening by circumstellar dust. We also compare our results with those of a narrow-band optical carbon star survey of WLM, and find the latter study sensitive to only 18% of the total AGB population. We detect objects with infrared fluxes consistent with them being mass-losing AGB stars, and derive a present day total mass-loss rate from the AGB stars of 0.7-2.4 x 10^(-3) solar masses per year. The distribution of mass-loss rates and bolometric luminosities of AGBs and red supergiants are very similar to those in the LMC and SMC and the empirical maximum mass-loss rate observed in the LMC and SMC is in excellent agreement with our WLM data.Comment: Accepted by ApJ, 34 pages, 13 figures, version with high-resolution figures available at: http://webusers.astro.umn.edu/~djackson

Neuronal Distribution in Colorectal Cancer:Associations With Clinicopathological Parameters and Survival

Over the past years, insights in the cancer neuroscience field increased rapidly, and a potential role for neurons in colorectal carcinogenesis has been recognized. However, knowledge on the neuronal distribution, subtypes, origin, and associations with clinicopathological characteristics in human studies is sparse. In this study, colorectal tumor tissues from the Netherlands Cohort Study on diet and cancer (n = 490) and an in-cohort validation population (n = 529) were immunohistochemically stained for the pan-neuronal markers neurofilament (NF) and protein gene product 9.5 (PGP9.5) to study the association between neuronal marker expression and clinicopathological characteristics. In addition, tumor and healthy colon tissues were stained for neuronal subtype markers, and their immunoreactivity in colorectal cancer (CRC) stroma was analyzed. NF-positive and PGP9.5-positive nerve fibers were found within the tumor stroma and mostly characterized by the neuronal subtype markers vasoactive intestinal peptide and neuronal nitric oxide synthase, suggesting that inhibitory neurons are the most prominent neuronal subtype in CRC. NF and PGP9.5 protein expression were not consistently associated with tumor stage, sublocation, differentiation grade, and median survival. NF immunoreactivity was associated with a worse CRC-specific survival in the study cohort (P = .025) independent of other prognostic factors (hazard ratio, 2.31; 95% CI, 1.33-4.03; P = .003), but these results were not observed in the in-cohort validation group. PGP9.5, in contrast, was associated with a worse CRC-specific survival in the in-cohort validation (P = .046) but not in the study population. This effect disappeared in multivariate analyses (hazard ratio, 0.81; 95% CI, 0.50-1.32; P = .393), indicating that this effect was dependent on other prognostic factors. This study demonstrates that the tumor stroma of CRC patients mainly harbors inhibitory neurons and that NF as a single marker is significantly associated with a poorer CRC-specific survival in the study cohort but necessitates future validation.</p

The Expression and Localization of N-Myc Downstream-Regulated Gene 1 in Human Trophoblasts

The protein N-Myc downstream-regulated gene 1 (NDRG1) is implicated in the regulation of cell proliferation, differentiation, and cellular stress response. NDRG1 is expressed in primary human trophoblasts, where it promotes cell viability and resistance to hypoxic injury. The mechanism of action of NDRG1 remains unknown. To gain further insight into the intracellular action of NDRG1, we analyzed the expression pattern and cellular localization of endogenous NDRG1 and transfected Myc-tagged NDRG1 in human trophoblasts exposed to diverse injuries. In standard conditions, NDRG1 was diffusely expressed in the cytoplasm at a low level. Hypoxia or the hypoxia mimetic cobalt chloride, but not serum deprivation, ultraviolet (UV) light, or ionizing radiation, induced the expression of NDRG1 in human trophoblasts and the redistribution of NDRG1 into the nucleus and cytoplasmic membranes associated with the endoplasmic reticulum (ER) and microtubules. Mutation of the phosphopantetheine attachment site (PPAS) within NDRG1 abrogated this pattern of redistribution. Our results shed new light on the impact of cell injury on NDRG1 expression patterns, and suggest that the PPAS domain plays a key role in NDRG1's subcellular distribution. © 2013 Shi et al

Multitarget Stool DNA Test Performance in an Average-Risk Colorectal Cancer Screening Population

INTRODUCTION: We set out to evaluate the performance of a multitarget stool DNA (MT-sDNA) in an average-risk colonoscopy-controlled colorectal cancer (CRC) screening population. MT-sDNA stool test results were evaluated against fecal immunochemical test (FIT) results for the detection of different lesions, including molecularly defined high-risk adenomas and several other tumor characteristics. METHODS: Whole stool samples (n = 1,047) were prospectively collected and subjected to an MT-sDNA test, which tests for KRAS mutations, NDRG4 and BMP3 promoter methylation, and hemoglobin. Results for detecting CRC (n = 7), advanced precancerous lesions (advanced adenoma [AA] and advanced serrated polyps; n = 119), and non-AAs (n = 191) were compared with those of FIT alone (thresholds of 50, 75, and 100 hemoglobin/mL). AAs with high risk of progression were defined by the presence of specific DNA copy number events as measured by low-pass whole genome sequencing. RESULTS: The MT-sDNA test was more sensitive than FIT alone in detecting advanced precancerous lesions (46% (55/119) vs 27% (32/119), respectively, P < 0.001). Specificities among individuals with nonadvanced or negative findings (controls) were 89% (791/888) and 93% (828/888) for MT-sDNA and FIT testing, respectively. A positive MT-sDNA test was associated with multiple lesions (P = 0.005), larger lesions (P = 0.03), and lesions with tubulovillous architecture (P = 0.04). The sensitivity of the MT-sDNA test or FIT in detecting individuals with high-risk AAs (n = 19) from individuals with low-risk AAs (n = 52) was not significantly different. DISCUSSION: In an average-risk screening population, the MT-sDNA test has an increased sensitivity for detecting advanced precancerous lesions compared with FIT alone. AAs with a high risk of progression were not detected with significantly higher sensitivity by MT-sDNA or FIT