Maximum parsimony tree of 12 aquaporin genes and the three clones (sb 005 09I19, CL138 and sb 006 05H07) (arrowed)

<p><b>Copyright information:</b></p><p>Taken from "Surviving extreme polar winters by desiccation: clues from Arctic springtail () EST libraries"</p><p>http://www.biomedcentral.com/1471-2164/8/475</p><p>BMC Genomics 2007;8():475-475.</p><p>Published online 21 Dec 2007</p><p>PMCID:PMC2246132.</p><p></p> Accession numbers: Human AQPs 1-12A: P29972, P41181, Q92482, P55087, P55064, Q13520, O14520, O94778, O43315, Q96PS8, Q8NBQ7, Q8IXF9; Mouse AQPs 1-12A (10 is absent): Q02013, P56402, Q8R2N1, P55088, Q9WTY4, Q8C4A0, O54794, P56404, Q9JJJ3, Q8BHH1, Q8CHJ2. Bovin AQPs 1, 3, 4: P47865, Q08DE6, O77750. Dog (CANFA) AQP 1: Q9N2J4. Sheep AQP5: Q866S3. Frog AQP (RANES) (Rana esculenta): P50501. Insect AQPs: Q0IG28, Q9NHW7: AEDAE: (yellow fever mosquito); Q23808: CICVR: (green leaf hopper); Q9V5Z7: DROME () (fruit fly); Q25074: HAEIX () (buffalo fly)

Alignment of the three putative aquaporin clones identified in the libraries

<p><b>Copyright information:</b></p><p>Taken from "Surviving extreme polar winters by desiccation: clues from Arctic springtail () EST libraries"</p><p>http://www.biomedcentral.com/1471-2164/8/475</p><p>BMC Genomics 2007;8():475-475.</p><p>Published online 21 Dec 2007</p><p>PMCID:PMC2246132.</p><p></p> Transmembrane domains are marked above the sequence (TM6 is only partial in both CL138 and sb_ 005_09I19). Red lines denote the two conserved NPA motifs of the aquaporin family. Only CL138 has conserved the site for N-glycosylation [31]. Red asterisks below the consensus line identify 13/14 amino acids conserved throughout the mammalian aquaporin family, as outlined in previous protein fragment analyses [59]. Percentage amino acid identities between the different springtail aquaporin clones. Figures in brackets are the percentage amino acid similarities. Each clone was clipped to the same size when performing the calculations

Example of GO molecular function (level 2) characterisation of the libraries, the data from all libraries combined is shown

<p><b>Copyright information:</b></p><p>Taken from "Surviving extreme polar winters by desiccation: clues from Arctic springtail () EST libraries"</p><p>http://www.biomedcentral.com/1471-2164/8/475</p><p>BMC Genomics 2007;8():475-475.</p><p>Published online 21 Dec 2007</p><p>PMCID:PMC2246132.</p><p></p