Uji Efektifitas Ekstras Etanol Buah Naga Putih (Hylocereus undatus) Terhadap Penurunan Kadar Asam Urat Darah Pada Mencit (Mus musculus)

Buah naga putih (Hylocereus undatus) mengandung flavonoid yang dapat menghambat kerja enzim xantin oksidase yang dapat mengurangi kadar asam urat. Penelitian bertujuan untuk mengetahui efektivitas ekstrak etanol buah naga putih dalam menurunkan kadar asam urat darah pada mencit yang diinduksi kafein secara oral dengan dosis 0,39 mg/25 g BB. Sampel buah naga putih diambil saat berumur 50-55 hari setelah muncul bunga. Pada uji efektifitas, hewan coba mencit dibagi menjadi lima kelompok perlakuan dan diinduksi dengan kafein yaitu kelompok kontrol negatif yang hanya diinduksi kafein, kelompok kontrol positif diberikan allopurinol  0,26 mg/25 g BB, kelompok dosis 0,455 mg/25 g BB, kelompok dosis 0,91 mg/25 g BB, kelompok dosis 1,82 mg/25 g BB ekstrak etanol buah naga putih. Pengukuran kadar asam urat darah dilakukan sebelum induksi (hari ke 1), sesudah induksi atau sebelum pemberian ekstrak (hari ke 6) dan sesudah pemberian ekstrak pada (hari ke 9, 12 dan 15). Hasil penelitian menunjukkan bahwa ekstrak etanol buah naga putih dosis 1,82 mg/25 g BB dapat menurunkan kadar asam urat darah pada mencit secara bermakna dibandingkan dengan dosis 0,455 mg dan dosis 0,91 mg/25 g BB, tetapi tidak berbeda bermakna dengan kontrol positif (allopurinol) dengan presentase penurunan sebesar 92,16%. Kata kunci: Ekstrak buah naga putih, asam urat darah, kafein, allopurinol.White dragon fruit (Hylocereus undatus) contains flavonoids that can inhibit the action of xanthine oxidase enzymes that can reduce uric acid levels. The aim of this research is to know the effectiveness of white dragon ethanol extract in decreasing uric acid level in caffeine-induced mice with dose 0.39 mg/25 g BW (body weight). White dragon fruit samples taken at the age of 50-55 days after the flower appeared. In the effectiveness test, animals were divided into five groups of treatments and induced by caffeine i.e. negatively control group induced only by caffeine, positive control group given allopurinol 0.26 mg/25 g BW, group of dose 0.455 mg/25 g BW, group of dose 0.91 mg/25 g BW, group of dose 1.82 mg/25 g BW ethanol extract of white dragon fruit. Measurements of blood uric acid levels were performed before induction (day 1), after induction or before administration of the extract (day 6) and after administration of the extract on (days 9, 12 and 15). The results showed that ethanol extract of white dragon fruit dose 1.82 mg/25 g BW could significantly lower blood uric acid levels in mice compared to dose 0.455 mg and dose 0.91 mg/25 g BW, but not significantly different with positive control (allopurinol) with percentage decrease of 92.16%. Keywords: White dragon fruit extract, blood uric acid, caffeine, allopurinol

KEBIASAAN MAKAN YANG BERHUBUNGAN DENGAN KESEHATAN REPRODUKSI REMAJA PUTRI DI KABUPATEN BOGOR

This research aims was to study (1) food habits of adolescent girls; (2) the adolescent girls’ efforts in taking care of and obtaining reproductive health; and (3) the relation between food habits, school level, parent’s education level, and social economics level of family towardreproductive health of adolescent girls. This research was a cross-sectional study executed in class level of 1 and 2 of SMA Negeri I and SMP Negeri I of Dramaga District, in Bogor Regency in which 3 classes of each level so that totalize 12 classes.  The number of subject was 169 adolescent girls. The data was collected by interviews and filling up questioners on May through June 2008. The research result showed that food habit between adolescent girls of SMP and SMA was not different and both have not yet meet PUGS, especially still less in number consume of rice, animal products and plain water. Strive in overcoming the sigh appearance nearing and during menstruation which is often done by adolescent girls were by keeping it quiet, body massages, and lay down. Variables of eating frequency together with habitual of eating fruits, animal products, and sour or hot food during menstruation are significantly related with reproductive health of adolescent girls.

EDUKASI MASYARAKAT DENGAN PENINGKATAN PENGETAHUAN KADER SEBAGAI UPAYA PENCEGAHAN DAN PENANGGULANGAN PENYAKIT TUBERKULOSIS (TB) PADA MASA PANDEMI DI KELURAHAN DURI KEPA JAKARTA BARAT

Tuberkulosis (TB) merupakan penyakit menular langsung yang disebabkan oleh bakteri Mycobacterium tuberculosis. Diperkirakan 95% kasus TB dan 98% kematian akibat TB di dunia, terjadi pada negara-negara berkembang. Indonesia merupakan negara ke-3 tertinggi penderita tuberkulosis. Hal tersebut mendorong pengendalian tuberkulosis nasional terus dilakukan dengan intensifikasi, akselerasi, ekstensifikasi dan inovasi program. Strategi pengendalian yang dapat dilakukan dengan memberikan penyuluhan tentang penyakit tuberkulosis (TB). Program pengabdian masyarakat ini dilaksanakan di Kelurahan Duri Kepa Jakarta Barat, dengan tujuan memberikan edukasi kepada masyarakat tentang pencegahan dan penanggulangan TB. Metode yang digunakan dalam pengabdian ini adalah metode edukasi langsung dengan memberikan penyuluhan kepada warga melalui ceramah dan diskusi dan metode edukasi tidak langsung dengan penyampaian informasi melalui media sosial. Hasil dari kegiatan ini adalah bertambahnya pemahaman masyarakat tentang pencegahan dan pengendalian TB dan menerapkannya dalam kehidupan sehari-har

PENGEMBANGAN MUTU PRODUK BIR PLETOK DI SENTRA INDUSTRI RUMAH TANGGA BIR PLETOK KELURAHAN KEDOYA SELATAN JAKARTA BARAT

Pada masyarakat etnis Betawi dikenal minuman ‘Bir Pletok’ yang sering dihidangkan dalam acara hajatan, acara penyambutan tamu, souvenir dan lain-lain. Minuman Bir Pletok tidak mengandung alkohol malahan baik untuk kesehatan karena mengandung bahan-bahan tanaman yang berkhasiat obat. Beberapa khasiat dari Bir Pletok adalah melancarkan peredaran darah, melindungi sistem pencernaan, menghangatkan badan, mengobati masuk angin, mengatasi sariawan, meredakan kram, meredakan migrain dan mengurangi gejala reumatik. Program pengabdian kepada masyarakat ini dilaksanakan pada mitra komunitas UMKM Bir Pletok Kedoya Selatan Jakarta Barat, dengan tujuan memberikan edukasi kepada masyarakat komunitas UMKM Bir Pletok Kedoya Selatan Jakarta Barat tentang proses produksi minuman herbal Bir Pletok sesuai HACCP/CPOTB dan standar Badan POM RI. Metode yang digunakan dalam pengabdian ini adalah memberikan edukasi dalam bentuk pendampingan yang terdiri atas penyuluhan dan pelatihan mengenai proses pengolahan Bir Pletok yang baik untuk mendapatkan produk Bir Pletok yang bermutu tinggi. Hasil dari kegiatan ini adalah peningkatan pengetahuan peserta mengenai pengembangan mutu minuman herbal Bir Pletok sebesar 95% dan mulai menerapkan pengetahuan yang didapatkan dalam kegiatan produksiny

Pendeteksian Ekspresi Biomarker ERK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positifCervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive

Pendeteksian Ekspresi Biomarker MNK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positif. Cervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive

OBESITY AND CARDIOVASCULAR DISEASE IN CHILDREN

Obesity is performed by genetic, environmental, and complex interaction between genetic and environmental factors.Fatness levels in obese children are indicative of increased risk for elevated blood pressure, cholesterol, and lipoproteinratios children and adolescents. Serum cholesterol and blood pressure are related to raise atherosclerotic lesion. Certaincardiovascular disease risk factors in obese children are related to the earliest stages of atherosclerosis disease. Obesechildren have the high risk factors of certain cardiovascular disease. Genetic factors affecting metabolic rate can besuccessfully managed by the introduction of environmental factors such as decrease caloric intake and increase physicalactivity. The treatment of obesity and of atherosclerotic patients should include dietary restriction (protein sparing modified fast and hypocaloric balanced diet), nutrition education, increased physical activity, behavior modification, and familial support. Success fully management of obesity can improve the quality of life and reduce the risk of morbidity and mortality associated with obesity.Keywords: Obesity, cardiovascular disease, energy balance, cholesterol, and lipoprotein

EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON THE IMMUNE SYSTEM

Protein-Energy Malnutrition (PEM) is believed to lead to an increased susceptibility to infection, or cause impaired immunity. Infection, occurring with malnutrition, is a major cause of morbidity in all age groups and is responsible fortwo-thirds of all death under 5 yr of age in developing countries. Many cells of the immune system are known to depend for their function on metabolic pathways that employ various nutrients as critical factors. The most consistent changes in immune competence in PEM are in cell-mediated immunity, the bactericidal function of neutrophils, the complement system, the secretory immunoglobin A, and antibody response.Keywords: protein-energy malnutrition, immune system, cell mediated immunity, amino acid deficiency, antibodyresponsesIntroductio

Protein-Energy Malnutrition (PEM) is Believed to Lead to an Increased Susceptibility to Infection, or cause Impaired Immunity

Infection, occurring with malnutrition, is a major cause of morbidity in all age groups and is responsible for two-thirds of all death under 5 yr of age in developing countries. Many cells of the immune system are known to depend for their function on metabolic pathways that employ various nutrients as critical factors. The most consistent changes in immune competence in PEM are in cell-mediated immunity, the bactericidal function of neutrophils, the complement system, the secretory immunoglobin A, and antibody response

KEBIASAAN MAKAN YANG BERHUBUNGAN DENGAN KESEHATAN REPRODUKSI REMAJA PUTRI DI KABUPATEN BOGOR

800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} This research aims was to study (1) food habits of adolescent girls; (2) the adolescent girls’ efforts in taking care of and obtaining reproductive health; and (3) the relation between food habits, school level, parent’s education level, and social economics level of family toward reproductive health of adolescent girls. This research was a cross-sectional study executed in class level of 1 and 2 of SMA Negeri I and SMP Negeri I of Dramaga District, in Bogor Regency in which 3 classes of each level so that totalize 12 classes.  The number of subject was 169 adolescent girls. The data was collected by interviews and filling up questioners on May through June 2008. The research result showed that food habit between adolescent girls of SMP and SMA was not different and both have not yet meet PUGS, especially still less in number consume of rice, animal products and plain water. Strive in overcoming the sigh appearance nearing and during menstruation which is often done by adolescent girls were by keeping it quiet, body massages, and lay down. Variables of eating frequency together with habitual of eating fruits, animal products, and sour or hot food during menstruation are significantly related with reproductive health of adolescent girls.   Key words: food habits, reproductive health, adolescent girl</em