Laju Pertumbuhan Dan Kelulusan Hidup Benih Clownfish (Amphiprion Ocellaris) Dengan Pakan Pelet Berbeda (Love Larva, NRD Dan TetraBits) Skala Laboratorium

Availability of juveniles Clownfish which have economic value is high enough on nature still is lacking so that the necessary technology laboratory scale hatchery Clownfish. Therefore, Balai Besar pengembangan Budidaya Laut Lampung apply the techniques and marine fish breeding and preservation of the adult Clownfish of marine fish resources and marine environment to meet those needs. Cultivation technique of feed fish is the most important factor in the growth juveniles Clownfish. This experiment was aimed to information about the effect of different pellets treatments (Love Larva, NRD, and TetraBits) on the graduation rate of growth and the survival rate juveniles Clownfish (Amphiprion ocellaris).The research was conducted for 43 days at the Balai Besar pengembangan Budidaya Laut Lampung on October 1 to November, 12 2011. The experiment was laboratory experimental conducted with rancangan acak lengkap (RAL) consisting of two types of feed pellets (NRD and TetraBits) and one control (Love Larva). Analysis of experiment using one-way ANOVA.The results showed that feeding pellets (Love Larva, NRD and TetraBits) had no effect on the length and weight growth (Count F < F Table 0.05). Obtained the highest survival rate feed B (TetraBits) is 98%. Keywords : Growth rate, Survival Rate, Amphiprion ocellaris, Love Larva, NRD and TetraBit

Laju Pertumbuhan Udang Windu (Penaeus Monodon), Ikan Bandeng (Chanos Chanos), Dan Rumput Laut (Eucheuma Cottonii, Gracilaria SP) Pada Budidaya Polikultur Dengan Padat Tebar Yang Berbeda Di Desa Sungai Lumpur Kabupaten OKI Sumatera Selatan

Pengembangan USAha perikanan di Kabupaten Ogan Komering Ilir sangat prospektif ditinjau dari segi aspek teknis, sosial, ekonomi, maupun sumberdaya yang tersedia. Kabupaten Ogan Komering Ilir khususnyadesa Simpang Tiga Kecamatan Tulung Selapan telah mengembangkan budidaya udang dan bandeng secara polikultur. Penelitian ini bertujuan untuk mengetahui laju pertumbuhan pada Udang Windu (Penaeus monodon), Ikan Bandeng (Chanos-chanos),dan Rumpu Laut Eucheumacotonii,Gracilaria sp) pada budidaya polikultur dengan padat tebar yang berbeda. Metode pengumpulan data yang diterapkan dalam penelitian ini adalah metode eksperimental dengan menggunakan rancangan acak kelompok (RAK) dengan menggunakan uji One Away Anova dengan 2 perlakuan dan 9 pengulangan. Data yang digunakan adalah data yang didapat langsung dari lapangan, dengan pengukuran yang dilakukan 10 hari sekali dan penimbangan berat selama 80 hari kedepan. Lajupertumbuhan berat rata-rata udang windu tertinggi dengan nilai 7,963% pada perlakuan A dan pada perlakuan B dengan nilai 7,667%. Laju pertumbuhan berat rata-rata ikan bandeng lebih tinggi pada perlakuan A dengan nilai 6,867%. Hal ini disebabkan karena dilakukan pemberian pakan, sedangkan perlakuan B dengannilai 6,528%. Laju pertumbuhan panjang udang windu telihat pada Tabel 7, terlihat jelas padat tebar yang tinggi memiliki panjang rata-rata yang lebih tinggi dengannilai 0,288 cm dan diikuti perlakuan B dengan nilai 0,236 cm. Laju pertumbuhan panjang rata-rata ikan bandengpadaTambak A lebih tinggi dengan nilai 0,284 cm dari perlakuan B dengannilai 0,231 cm

Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma Cottoni Terhadap Bakteri Escherichia Coli, Staphylococcus Aureus, Vibrio Cholera Dan Salmonella Typhosa

Seaweed (Eucheuma cottonii) is one of the marine biological resources that contain flavonoid compounds as antibacterial agents. In a previous study the antibacterial Eucheuma cottonii, had been performed by dipping the disc paper into antibacterial extract, while in this study the antibacterial extract in exact volume will be dropped onto the disc paper, so we will know the volume certainty. The aims of the study were to determine the antibacterial activity and the minimum inhibitory concentration of the extract of Eucheuma cottonii test bacteria by using methanol solvent. The study was conducted in September-October 2012. In this study, seaweed extract made by using maceration method while the antibacterial activity testing by using agar diffusion method. The study results showed that the extract of Eucheuma cottonii antibacterial activity inhibited the growth of Staphylococcus aureus with inhibition zone diameter 17.33 mm, Escherichia coli with inhibition zone diameter 16,33 mm, Vibrio cholera with inhibition zone diameter 13,67 mm and Salmonella typhosa with inhibition zone diameter 11,67 mm. Minimum inhibitory concentration of Eucheuma cottonii to Escherichia coli, Staphylococcus aureus and Vibrio cholera were at 1% concentration while Salmonella typhosa was at 5 % concentration

Penambahan Serbuk Buah Avicennia Marina Terhadap Laju Pertumbuhan Ikan Kakap Putih (Lates Calcarifer) Pada Skala Laboratorium

The success of increased production of snapper feed depends on many factors, but the nutrients from food is a major factor in intensive care because of feed shortages would hamper growth and hasten the death of the fish are kept. The purpose of this study to look at graduation rates of life (Survival rate) perch (Lates calcarifer) and growth of perch (Lates calcarifer) after being given the addition of fruit powder A. marina. The study was conducted on May 16 to June 20 2011. Metode experimental method used is a laboratory. Experiments conducted it this study is 4 (four) treated with 3(three) times the loop a follows: P1 (control), P2 (10 gr/100 g feed), P3 (20 gr/100 g feed), and P4 (30g / 100 g feed). Highest passing rate is 93.33% of life on treatment P3 (20/100 g feed) and P1 treatment (control).Treatment of P3 (20 gr/100 g feed) showed a daily weight growth rate (%) and growth rate of absolute weight (g) the highest perch is 4.05 % and 0.67 grams, while the length of daily growth rate (%) highof 8.36 % in the treatment of P3 (20 gr/100 g of feed) ,and the rate of growth in absolute length (cm) high of 0.58 cm in the treatment of P3 (20 gr/100 g feed) and P4 (30 gr/100 g of feed)

Semi-quantitative, duplexed qPCR assay for the detection of leishmania spp. using bisulphite conversion technology

© 2019 by the authors. Leishmaniasis is caused by the flagellated protozoan Leishmania, and is a neglected tropical disease (NTD), as defined by theWorld Health Organisation (WHO). Bisulphite conversion technology converts all genomic material to a simplified form during the lysis step of the nucleic acid extraction process, and increases the efficiency of multiplex quantitative polymerase chain reaction (qPCR) reactions. Through utilization of qPCR real-time probes, in conjunction with bisulphite conversion, a new duplex assay targeting the 18S rDNA gene region was designed to detect all Leishmania species. The assay was validated against previously extracted DNA, from seven quantitated DNA and cell standards for pan-Leishmania analytical sensitivity data, and 67 cutaneous clinical samples for cutaneous clinical sensitivity data. Specificity was evaluated by testing 76 negative clinical samples and 43 bacterial, viral, protozoan and fungal species. The assay was also trialed in a side-by-side experiment against a conventional PCR (cPCR), based on the Internal transcribed spacer region 1 (ITS1 region). Ninety-seven percent of specimens from patients that previously tested positive for Leishmania were positive for Leishmania spp. with the bisulphite conversion assay, and a limit of detection (LOD) of 10 copies per PCR was achieved, while the LOD of the ITS1 methodology was 10 cells/1000 genomic copies per PCR. This method of rapid, accurate and simple detection of Leishmania can lead to improved diagnosis, treatment and public health outcomes

The Effect of Blanching Methods and Extractions on Quality of Edamame Milk Product

This research was conducted with the aim to know the study the effect of blanching method and extraction on edamame milk characteristics. The design used in this study was a Randomized Block Design (RBD) with a factorial pattern consisting of two factors (blanching methods and extraction) and two replications. The analysis is done by objective and subjective. The actual analysis including of water content, pH, total soluble solids (TSS), protein content, ash content, and shelf-life. Individual observations include assessment of color, viscosity, flavor, and overall reception using the scoring method by SNI 3141.1: 2011. The result showed that the blanching method and extraction could maintain the characteristics up to 18 days of storage with features: water content 90.10%, pH 7.09, TSS, 15,90, protein content 1.25 (below standard quality), ash content 0.48, 5.93 colors, 5.60 flavor, 5.73 viscosity, 5.87 taste, and reception overall (rather like-like)

Reliable intraocular pressure measurement using automated radio-wave telemetry

Purpose To present an autonomous intraocular pressure (IOP) measurement technique using a wireless implantable transducer (WIT) and a motion sensor. Methods: The WIT optical aid was implanted within the ciliary sulcus of a normotensive rabbit eye after extracapsular clear lens extraction. An autonomous wireless data system (AWDS) comprising of a WIT and an external antenna aided by a motion sensor provided continuous IOP readings. The sensitivity of the technique was determined by the ability to detect IOP changes resulting from the administration of latanoprost 0.005% or dorzolamide 2%, while the reliability was determined by the agreement between baseline and vehicle (saline) IOP. Results: On average, 12 diurnal and 205 nocturnal IOP measurements were performed with latanoprost, and 26 diurnal and 205 nocturnal measurements with dorzolamide. No difference was found between mean baseline IOP (13.08±2.2 mmHg) and mean vehicle IOP (13.27±2.1 mmHg) (P=0.45), suggesting good measurement reliability. Both antiglaucoma medications caused significant IOP reduction compared to baseline; latanoprost reduced mean IOP by 10% (1.3±3.54 mmHg; P<0.001), and dorzolamide by 5% (0.62±2.22 mmHg; P<0.001). Use of latanoprost resulted in an overall twofold higher IOP reduction compared to dorzolamide (P<0.001). Repeatability was ±1.8 mmHg, assessed by the variability of consecutive IOP measurements performed in a short period of time (≤1 minute), during which the IOP is not expected to change. Conclusion: IOP measurements in conscious rabbits obtained without the need for human interactions using the AWDS are feasible and provide reproducible results