Depression and family support in breast cancer patients

MTS, migration and invasion assays in DCIS.COM cells that were previously transduced with scrambled control (Control) or BCL9 KD shRNA. The control cells and BCL9 KD cells were re-transduced with empty vector (EV), BCL9 overexpression (BCL9-OE) and BCL9 KD. BCL9-OE was achieved by transduction using the PCDH-BCL9 (BCL9-OE) acquired from Dr. Carrasco [11]. A Western blot analysis was performed using anti-BCL9, anti-vimentin, anti-E-cadherin antibodies, and anti-β-actin as a loading control. B MTS assay on control cells transduced with EV (control + EV), or BCL9-OE (control + BCL9-OE), BCL9-KD transduced with EV (BCL9 KD + EV), and BCL9-KD transduced with BCL9-OE (BCL9 KD + BCL9-OE). Bar graphs represent mean absorbance at 490 nm normalized to control ± standard error of the mean (SEM) (n = 6). C, D Representative images of the migration and invasion assays. Bar graph represents percent area of cells migrated (left) and invaded (right) under the membrane after 24 h. Invasion and migration were determined by ImageJ analysis of microscopic images per sample, the data are mean values normalized to control ± SEM (n = 3). E TopFlash and FopFlash reporter activity in DCIS.COM transduced as above that were either treated with Wnt3A or control conditioned medium (CM). Data represent mean ± SEM (n = 3, letters indicate statistically significant difference). (PDF 964 kb

Rotala mexicana Cham. et Schltdl.

原著和名: ミヅマツバ科名: ミソハギ科 = Lythraceae採集地: 群馬県 館林市 多々良沼付近 (上野 館林市 多々良沼付近)採集日: 1986/10/10採集者: 萩庭丈壽整理番号: JH024411国立科学博物館整理番号: TNS-VS-97441

Additional file 1: Table S1. of Race-associated biological differences among luminal A and basal-like breast cancers in the Carolina Breast Cancer Study

Gene expression by participant and clinical characteristics from CBCS Phase 3, 2008–2013. Table S2. RNA counts overall and by race and estrogen receptor (ER) status from CBCS Phase 3, 2008–2013. Table S3. Gene expression and risk of recurrence among women with ER+/HER2– breast cancer from CBCS Phase 3, 2008–2013. (DOCX 81 kb

Additional file 1: Table S1. of Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet

Compositions of the diets. (PDF 118 kb

Additional file 3: Table S3. of Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet

Ontology analysis of short-latency tumors that cluster together. (PDF 38 kb

Additional file 5: Figure S2. of Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet

Effects of diet treatments on blood levels of glucose and insulin. BALB/c mice started on HFD or LFD at 3 weeks of age were switched to LFD or HFD, respectively, at 9 weeks of age. Blood levels of glucose (a, c) and insulin (b, d) were measured at 4 weeks post diet switches (a, b) or in tumor-bearing mice (c, d). The bars represent mean ± SEM for samples at 4 weeks after diet switches and from tumor-bearing mice (n = 5 for all groups). No significant differences were detected. (PDF 653 kb

Additional file 1: of Intratumoral heterogeneity as a source of discordance in breast cancer biomarker classification

ER and PR expression levels among cases with discordant biomarker status between cores, restricting to cases with negative (<1 %) ER and PR expression. Cases are ordered on the X-axis by case-level positivity status. Individual cores are represented by black crosses, and case-level positivity status is represented by red crosses. (TIF 452 kb

MOESM3 of Weight loss reduces basal-like breast cancer through kinome reprogramming

Additional file 3. Kinome profiling revealed significant regulation of pathways by HFD that were reversed with weight loss. A and b. Quantitative comparison of kinases in unaffected mammary tissues from mice using MIB/MS was conducted. Legend indicates three iTRAQ runs with 2–4 samples pooled per group per run. The graphs indicates quantitative changes in kinase activity as a ratio of mice fed 60 % (a) or 60–10 % (b) diet relative to mice fed 10 % diet group. Ratio <1 denotes decreased kinase activity and >1 increased kinase activity. Kinase families are indicated (AGC: Containing PKA, PKG, PKC families; CAMK: Calcium/calmodulin-dependent protein kinase; CK1: Casein kinase 1; CMGC: Containing CDK, MAPK, GSK3, CLK families; STE: Homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases; TK: Tyrosine kinase; TKL: Tyrosine kinase-like). c. Mean kinase activity is reported for mice fed 60 % diet (dark grey) or 60–10 % diet (light grey) compared to mice on 10 % diet group. Error bars are not indicated for clarity. Statistically significant comparisons are reported in Fig. 5d

Additional file 1: Table S1. of Relationship between crown-like structures and sex-steroid hormones in breast adipose tissue and serum among postmenopausal breast cancer patients

Select patient and clinical characteristics of women included in this study as well as the relationship between the number of CD68-positive macrophages and number of CLS (per unit area of fat) and hormones. (DOCX 25 kb

Additional file 1: of A survey of microRNA single nucleotide polymorphisms identifies novel breast cancer susceptibility loci in a case-control, population-based study of African-American women

Table S1. Association of the top seven miRNA SNPs from the full analyses with p < 5 × 10−6 in case versus control and case-only subtype analyses. (XLSX 72 kb