Esophageal eosinophil level in an allergen-induced experimental eosinophil esophagitis model in miR-21^+/+ and miR-21^−/− mice.

<p>Eosinophil levels in the esophagus were determined by morphometric analysis following anti-MBP staining. N = 5–6 mice per group. Data are represented as mean ± S.E.M. Data are representative of 2 independent experiments.</p

Levels of apoptosis in the eosinophil progenitor culture from the miR-21^+/+ and miR-21^−/− mice.

<p>Levels of Annexin V and 7AAD staining during eosinophil differentiation culture as determined by FACS. The viable cells are AnnexinV⁻ 7AAD⁻. The early apoptotic cells are AnnexinV⁺ 7AAD⁻. The late apoptotic cells are AnnexinV⁺ 7AAD⁺. Data are representative of 3 independent experiments.</p

MiR-21 is induced during eosinophil differentiation.

<p>(A) Purity of cultured eosinophils at day 14. Eosinophils are identified as CCR3⁺Siglec-F⁺ cells. (B) Levels of miR-21 during the eosinophil differentiation culture determined by qPCR normalized to U6 small nuclear RNA. N = 3 per group; data are represented as mean ± S.E.M. Data are representative of 3 independent experiments.</p

Growth of eosinophil progenitor cells from miR-21^−/− mice and miR-21^+/+ controls during the ex vivo eosinophil culture.

<p>Total cell number of (A) eosinophil and (B) neutrophil cultures derived from miR-21^+/+ and miR-21^−/− mice is shown. N = 6 per group; data are represented as mean ± S.E.M. **: p<0.01. (C) Morphology of miR-21^+/+ and miR-21^−/− cultured eosinophils at day 12 determined by Diff-Quik staining. Data are representative of 3 independent experiments for panels A and C; data are representative of 2 independent experiments for panel B.</p