MOESM1 of Does perfectionism in bipolar disorder pedigrees mediate associations between anxiety/stress and mood symptoms?

Additional file 1. Supplementary separate analyses for probands and non-affected family members

Framework domains, illustrative quotes, and recommendation statements.

<p>Framework domains, illustrative quotes, and recommendation statements.</p

Multi-staged methods.

<p>Multi-staged methods.</p

Engaged scholarship ethics framework.

<p>Note. Vision of just and equitable research is the overarching domain with three additional domains: relationship dynamics, accountability, and community-informed risks/benefits assessment. The outer circle represents the four cross-cutting considerations: capacity building, translation for improved health, power/hierarchy, and individual vs. group concerns: collective ethos.</p

Vibratome sections of E13.5 <i>TOPGAL</i>, <i>BATGAL</i> and <i>Axin2^LacZ</i> lungs.

<p><i>TOPGAL</i> is expressed in both epithelium and mesenchyme at the level of the bronchi (<b>A, B</b>) and restricted to the epithelium in the distal lung (<b>C</b>). <i>BATGAL</i> “salt and pepper” expression is found in a heterogeneous fashion in the lung (<b>D</b>). The expression is restricted to the mesenchyme adjacent to the bronchial epithelium (<b>E</b>). In the distal lung, <i>BATGAL</i> is sporadically expressed in the epithelium (<b>F</b>). <i>Axin2^LacZ</i> is found exclusively in the mesenchyme adjacent to the epithelium at the bronchial level (<b>G, H</b>). While in the distal lung, LacZ expression is found mainly in the epithelium and at lower level in the mesenchyme (<b>I</b>).</p

Corn oil and Naphthalene-treated whole mount left lungs cleared with Benzyl benzoate.

<p>In the adult lung, <i>TOPGAL</i> is strongly expressed in the epithelium (<b>A, B</b>) and decreases slightly after naphthalene injury (<b>C, D</b>). <i>BATGAL</i> expression is totally absent in the adult lung (<b>E, F</b>) and a discrete expression appears after injury (<b>G, H</b>). While a homogenous <i>Axin2^LacZ</i> expression is present throughout the adult lung (<b>I, J</b>), a marked increase surrounding the bronchial epithelium is observed after injury (<b>K, L</b>).</p

LacZ expression in whole embryos of <i>TOPGAL</i>, <i>BATGAL</i> and <i>Axin2^LacZ</i> mice.

<p>(<b>A</b>) E11.5 <i>TOPGAL</i> embryo shows staining in the forebrain, the nasal process, the inner ear, the apical ectodermal ridge (AER) of the limb, the epithelium of the mammary placode, the somites and the tip of the tail. (<b>B</b>) E12.5 <i>TOPGAL</i> embryo shows LacZ expression in ectodermal appendages, the whisker placodes in the nasal region as well as the AER, the mammary buds in between the limbs and discrete mesenchymal condensation within the limbs. (<b>C</b>) E11.5 <i>BATGAL</i> embryo shows expression throughout the embryo with a “salt and pepper” pattern with higher signals in ectodermal domains such as the nasal process, the forebrain, the AER and the tip of the tail. (<b>D</b>) At E12.5, the <i>BATGAL</i> embryo still shows “salt and pepper” expression throughout the embryo. LacZ expression is found in the mammary buds but significant staining was also found in the surrounding tissue. (<b>E</b>) E11.5 <i>Axin2^LacZ</i> embryo shows homogenous LacZ expression throughout the embryo with higher expression in the AER and the developing mammary placode. (<b>F</b>) At E12.5, individualized whisker placodes as well as mammary buds and the AER are clearly positive for <i>Axin2^LacZ</i>. In addition, strong LacZ expression is found in the developing ear and in the somites.</p

CC10 and LacZ staining in the proximal lungs after naphthalene injury.

<p>CC10 and LacZ co-staining in <i>TOPGAL</i> control lungs at low (<b>A</b>) and high (<b>B</b>) magnification show strong expression of <i>TOPGAL</i> in the CC10-positive cells. A decrease in the Clara cells after naphthalene injury is shown in the proximal airways (<b>C, D</b>). <i>BATGAL</i> expression is not detected in the airways (<b>E, F</b>) but at discrete spots in the bronchial epithelium after injury (<b>G, H</b>). <i>Axin2^LacZ</i> sections showed very low level staining throughout the lung in the conducting airways of the control adult lungs (<b>I, J</b>), and an increased expression after injury (<b>K, L</b>). Scale bars are 100 µm.</p

SMA and LacZ staining in the proximal lung compartment after naphthalene injury.

<p>Co-staining of SMA and LacZ at low (<b>A</b>) and high (<b>B</b>) magnifications showed co-localization in the PBSMCs of control and naphthalene-treated lungs (<b>C, D</b>). <i>BATGAL</i> expression is not detected in the proximal PBSMCs in the control lung (<b>E, F</b>) and naphthalene-treated lungs (<b>G, H</b>). <i>Axin2^LacZ</i> sections showed low level staining in the PBMSCs of the control adult lungs (<b>I, J</b>) whereas LacZ expression is drastically upregulated after injury as pointed out with the arrows (<b>K, L</b>). Dotted lines show the basal membrane separating the epithelium from the PSMC layer. Scale bars are 100 µm.</p

CC10 and LacZ staining in the distal lung compartment after naphthalene injury.

<p>CC10 and LacZ co-staining in <i>TOPGAL</i> control lungs at low (<b>A</b>) and high (<b>B</b>) magnification show strong expression of <i>TOPGAL</i> in the CC10-positive cells. A decrease in the Clara cells after naphthalene injury is shown in the distal airways (<b>C, D</b>). <i>BATGAL</i> expression is not detected in the distal airways (<b>E, F</b>) of adult control lungs, and still absent from the epithelium (<b>G, H</b>) in the distal compartment of the naphthalene-treated lungs. <i>Axin2^LacZ</i> sections showed very low level staining in the distal airways of the control adult lungs (<b>I, J</b>), and an increased expression after injury (<b>K, L</b>). Scale bars are 100 µm.</p