Use of a Cholera Rapid Diagnostic Test during a Mass Vaccination Campaign in Response to an Epidemic in Guinea, 2012

During the 2012 cholera outbreak in the Republic of Guinea, the Ministry of Health, supported by Médecins Sans Frontières - Operational Center Geneva, used the oral cholera vaccine Shanchol as a part of the emergency response. The rapid diagnostic test (RDT) Crystal VC, widely used during outbreaks, detects lipopolysaccharide antigens of Vibrio cholerae O1 and O139, both included in Shanchol. In the context of reactive use of a whole-cell cholera vaccine in a region where cholera cases have been reported, it is essential to know what proportion of vaccinated individuals would be reactive to the RDT and for how long after vaccination

Rapid diagnostic test results in vaccinated participants by day of follow-up, Kabak, 2012.

1<p>The total is the sum of A+B+C+D.</p>2<p>The proportion is the result of the formula (a.1/(A+B))*100.</p>3<p>97.5% Confidence Interval, one-sided.</p

Rapid diagnostic test results performed in vaccine and bacterial suspension dilutions, Pasteur Institute, 2012.

<p>Intensity of the positive line: (+) very weak positive; (++) weak positive; (+++) positive.</p><p>Negative result: (−).</p

Linear regression model of time to become negative by age and sex, Kabak, 2012.

1<p>The coefficient shows the increase in days in the time to become negative per year of age.</p>2<p>The coefficient shows the increase in days in the time to become negative for males compared to females.</p

Study participants, exclusions and follow-up results, Kabak, 2012.

<p>Study participants, exclusions and follow-up results, Kabak, 2012.</p