Racial/Ethnic Disparities in Diabetes Care and Outcomes: A Mixed Methods Study

Limited research has examined racial/ethnic differences in diabetes care and outcomes among primary care patients. This study examined racial/ethnic differences in diabetes care and outcomes among an ambulatory patient population and explored patient perceptions of the patient-provider relationship to inform strategies to improve care delivery. Using data from 62,149 adults with diabetes who received care within Atrium Health in 2013, regression models assessed associations between race/ethnicity and the following outcomes: glycated hemoglobin (HbA1c) tests, low density lipoprotein (LDL) and blood pressure (BP) screening, foot and eye exams, and HbA1c, LDL, and BP control. Eleven patients with diabetes and uncontrolled hypertension participated in three focus groups about their perceptions of the patient-provider relationship. Compared to non-Hispanic Whites, non-Hispanic Blacks had 22% to 73% higher odds of receiving screenings (HbA1c, LDL, BP, foot and eye exams;

Proteomics of herpes simplex virus infected cell protein 27: association with translation initiation factors

AbstractThe herpes simplex virus (HSV) immediate early ICP27 protein plays an essential role in stimulating viral early and late gene expression. ICP27 appears to be multifunctional in that it has been reported to stimulate viral late gene transcription, polyadenylation site usage, and RNA export. We report here on proteomic studies involving immunoprecipitation of ICP27 and mass spectrometric identification of co-precipitated proteins. These studies show an association of ICP27 with the cellular translation initiation factors poly A binding protein (PABP), eukaryotic initiation factor 3 (eIF3), and eukaryotic initiation factor 4G (eIF4G) in infected cells. Immunoprecipitation-western blot studies confirmed these associations. Finally, purified MBP-tagged ICP27 (MBP-27) can interact with eIF3 subunits p47 and p116 in vitro. These results suggest that ICP27 may also play a role in stimulating translation of certain viral and host mRNAs and/or in inhibiting host mRNA translation

A century of anthropogenic environmental change in tropical Asia: multi-proxy palaeolimnological evidence from Singapore’s Central Catchment

The environmental ramifications of rapid development on the functioning of warm tropical freshwater ecosystems are poorly understood. Here, a multi-proxy palaeolimnological approach is used to examine the nature and degree of anthropogenic environmental change in a tropical lowland reservoir in Singapore. Singapore has undergone a dramatic transformation over the past century, transitioning from a country with a largely agrarian landscape to one that is highly urbanised. Two radiometrically dated sediment cores were retrieved from one of the country’s oldest reservoirs and analysed for spheroidal carbonaceous particles (SCPs), mercury (Hg), atomic carbon (C) and nitrogen (N), stable C and N isotopes (δ13C and δ15N), and diatoms. The sedimentary data show clear evidence of atmospheric pollution and nutrient enrichment as a result of human activities in Singapore and the region. During the early stages of Singapore’s development (1900s–1960s), the reservoir was minimally impacted and characterised by oligotrophic conditions. As the country began to industrialise and urbanise (1970s–1990s), the SCP data indicate increasing contamination by air pollutants derived from domestic sources of fossil fuel combustion, while the diatom, C/N, δ13C, and δ15N data suggest that the reservoir was becoming more productive, possibly from N depositions arising from an increase in electricity generation and a rapid expansion in transport infrastructure in Singapore. As the pace of development in Singapore slowed down (1990s–the present), the sedimentary data collectively indicate increasing depositions of atmospheric pollutants and nutrient enrichment mediated by a warming climate. A substantial component of increased atmospheric pollution is likely to be of distal, and thus transboundary, origin

De novo reefs: Fish habitat provision by oyster aquaculture varies with farming method

Aquaculture industries have the capacity to produce positive ecosystem service benefits, such as the provision of habitat to wild animals. Oyster cultivation is the oldest and largest aquaculture industry in south-eastern Australia. Oyster spat are grown to marketable size in rack-and-rail (‘racks’) or longline-and-basket (‘baskets’) configurations, which add structure to estuarine waters. This study assessed: (1) how the fish communities associated with oyster farms vary with production method; (2) how communities of fish utilise oyster infrastructure, as compared to adjacent natural habitats; and (3) whether oyster infrastructure can serve as de facto oyster reefs by supporting similar fish communities. Remote underwater video surveys, conducted during summer and winter of 2 study years, revealed that fish observations and species richness were generally greater for rack than basket cultivation. Both types of oyster farms supported at least as many species of fish as adjacent natural habitats, including oyster reef, seagrass, mangrove and bare sediment. Fish communities were, in general, most similar between racks and baskets and most dissimilar between racks and bare sediments. Oyster farms supported species of fish otherwise limited to habitats with wild oysters, and unique harvested fish species were observed more frequently at racks. Fish use of oyster-growing infrastructure for foraging and shelter mirrored use of natural biogenic habitats. Overall, this study suggests that the oyster aquaculture infrastructure can support fish communities with species composition similar to those of natural biogenic habitats, although this service is dependent on farming method. Ecosystem services provided by aquaculture should be considered in estuarine habitat enhancement, conservation and restoration

Invited Perspective: Building sustainable and resilient communities – Recommended actions for natural hazard scientists

Reducing disaster risk is critical to securing the ambitions of the Sustainable Development Goals (SDGs), and natural hazard scientists make a key contribution to achieving this aim. Understanding Earth processes and dynamics underpins hazard analysis, which (alongside analysis of other disaster risk drivers) informs the actions required to manage and reduce disaster risk. Here we suggest how natural hazard research scientists can better contribute to the planning and development of sustainable and resilient communities through improved engagement in disaster risk reduction (DRR). Building on existing good practice, this perspective piece aims to provoke discussion in the natural hazard science community about how we can strengthen our engagement in DRR. We set out seven recommendations for enhancing the integration of natural hazard science into DRR: (i) characterise multi-hazard environments, (ii) prioritise effective, positive, long-term partnerships, (iii) understand and listen to your stakeholders, (iv) embed cultural understanding into natural hazards research, (v) ensure improved and equitable access to hazards information, (vi) champion people-centred DRR (leaving no one behind), and (vii) improve links between DRR and sustainable development. We then proceed to synthesise key actions that natural hazards scientists and research funders should consider taking to improve education, training, and research design, and to strengthen institutional, financial and policy actions. We suggest that these actions should help to strengthen the effective application of natural hazards science to reduce disaster risk. By recognising and taking steps to address the issues raised in these recommendations, we propose that the natural hazard science community can more effectively contribute to the inter/transdisciplinary, integrated work required to improve DR

Ancient expansion of the Hox cluster in Lepidoptera generated four homeobox genes implicated in extra-embryonic tissue formation

Gene duplications within the conserved Hox cluster are rare in animal evolution, but in Lepidoptera an array of divergent Hox-related genes (Shx genes) has been reported between pb and zen. Here, we use genome sequencing of five lepidopteran species (Polygonia c-album, Pararge aegeria, Callimorpha dominula, Cameraria ohridella, Hepialus sylvina) plus a caddisfly outgroup (Glyphotaelius pellucidus) to trace the evolution of the lepidopteran Shx genes. We demonstrate that Shx genes originated by tandem duplication of zen early in the evolution of large clade Ditrysia; Shx are not found in a caddisfly and a member of the basally diverging Hepialidae (swift moths). Four distinct Shx genes were generated early in ditrysian evolution, and were stably retained in all descendent Lepidoptera except the silkmoth which has additional duplications. Despite extensive sequence divergence, molecular modelling indicates that all four Shx genes have the potential to encode stable homeodomains. The four Shx genes have distinct spatiotemporal expression patterns in early development of the Speckled Wood butterfly (Pararge aegeria), with ShxC demarcating the future sites of extraembryonic tissue formation via strikingly localised maternal RNA in the oocyte. All four genes are also expressed in presumptive serosal cells, prior to the onset of zen expression. Lepidopteran Shx genes represent an unusual example of Hox cluster expansion and integration of novel genes into ancient developmental regulatory networks

Metalanguage in L1 English-speaking 12-year-olds: which aspects of writing do they talk about?

Traditional psycholinguistic approaches to metalinguistic awareness in L1 learners elicit responses containing metalanguage that demonstrates metalinguistic awareness of pre-determined aspects of language knowledge. This paper, which takes a more ethnographic approach, demonstrates how pupils are able to engage their own focus of metalanguage when reflecting on their everyday learning activities involving written language. What is equally significant is what their metalanguage choices reveal about their understanding and application of written language concepts