500 años de la fundación del Primer Ayuntamiento Continental de las Américas

Entre el primer tercio y la mitad del año 2019, diversas dependencias y organismos públicos mexicanos celebraron el quincuagésimo aniversario de la fundación del ayuntamiento de la Villa Rica de la Vera Cruz. Con la afirmada trascendencia del suceso aludido, el Instituto Nacional de Administración Pública, fiel a su misión de difundir el conocimiento disciplinar, fue el primero en celebrar su quincuagésimo aniversario. El Seminario 500 años de la fundación del primer ayuntamiento continental de las Américas, realizado los días 9 y 10 de abril de 2019, propuso para los gobiernos municipales mexicanos una nueva visión para su organización y para su progreso. Se desarrolló en cinco mesas: 1) origen, 2) siglo XIX y Revolución Mexicana, 3) municipio urbano, 4) futuro del municipio 1, y 5) futuro del municipio 2. El primer capítulo de este libro reproduce las palabras del discurso inaugurar el seminario. Los restantes 18 capítulos corresponden a contribuciones escritas de aquellos participantes distinguidos del evento

A Molecular Characterization of the Allelic Expression of the BRCA1 Founder Δ9–12 Pathogenic Variant and Its Potential Clinical Relevance in Hereditary Cancer:International Journal of Molecular Sciences

Hereditary breast and ovarian cancer (HBOC) syndrome is a genetic condition that increases the risk of breast cancer by 80% and that of ovarian cancer by 40%. The most common pathogenic variants (PVs) causing HBOC occur in the BRCA1 gene, with more than 3850 reported mutations in the gene sequence. The prevalence of specific PVs in BRCA1 has increased across populations due to the effect of founder mutations. Therefore, when a founder mutation is identified, it becomes key to improving cancer risk characterization and effective screening protocols. The only founder mutation described in the Mexican population is the deletion of exons 9 to 12 of BRCA1 (BRCA1Δ9–12), and its description focuses on the gene sequence, but no transcription profiles have been generated for individuals who carry this gene. In this study, we describe the transcription profiles of cancer patients and healthy individuals who were heterozygous for PV BRCA1Δ9–12 by analyzing the differential expression of both alleles compared with the homozygous BRCA1 control group using RT–qPCR, and we describe the isoforms produced by the BRCA1 wild-type and BRCA1Δ9–12 alleles using nanopore long-sequencing. Using the Kruskal–Wallis test, our results showed a similar transcript expression of the wild-type allele between the healthy heterozygous group and the homozygous BRCA1 control group. An association between the recurrence and increased expression of both alleles in HBOC patients was also observed. An analysis of the sequences indicated four wild-type isoforms with diagnostic potential for discerning individuals who carry the PV BRCA1Δ9–12 and identifying which of them has developed cancer