Detection of Favorable QTL Alleles and Candidate Genes for Lint Percentage by GWAS in Chinese Upland Cotton

Improving cotton yield is a major breeding goal for Chinese upland cotton. Lint percentage is an important yield component and a critical economic index for cotton cultivars, and raising the lint percentage has a close relationship to improving cotton lint yield. To investigate the genetic architecture of lint percentage, a diversity panel consisting of 355 upland cotton accessions was grown, and the lint percentage was measured in four different environments. Genotyping was performed with specific-locus amplified fragment sequencing (SLAF-seq). Twelve single-nucleotide polymorphisms (SNPs) associated with lint percentage were detected via a genome-wide association study (GWAS), in which five SNP loci distributed on chromosomes At3 (A02) and At4 (A08) and contained two major-effect QTLs, which were detected in the best linear unbiased predictions (BLUPs) and in more than three environments simultaneously. Furthermore, favorable haplotypes (FHs) of two major-effect QTLs and 47 putative candidate genes in the two linkage disequilibrium (LD) blocks of these associated loci were identified. The expression levels of these putative candidate genes were estimated using RNA-seq data from ten upland cotton tissues. We found that Gh_A02G1268 was very highly expressed during the early fiber development stage, whereas the gene was poorly expressed in the seed. These results implied that Gh_A02G1268 may determine the lint percentage by regulating seed and fiber development. The favorable QTL alleles and candidate genes for lint percentage identified in this study will have high potential for improving lint yield in future Chinese cotton breeding programs

Untersuchungen uber das Schicksal der in rohes und gekochtes Brunnenwasser gemischten pathogenen Bakterien

Der Verfasser hat Untersuchungen uber die Lebensdauer von pathogenen Darmbakterien, die in rohes und gekochtes Brunnenwasser gemischt wurden, durchgefuhrt. Er hat 5 Brunnenwasser untersucht mit B. typhosus, B. paratyphosus A u. B, B. dysenteriae Komagome A u. B, B. dysenteriae Shiga; die Stamme erhielt Verf. aus dem Staatlichen Institut fur Infektionskrankheiten und aus dem bakteriologischen Institut zu Chiba. Die Ergebnisse sind folgende: 1) war die Menge der verwandten Bakterien klein (ca. 1/3000 Ose in 10 cc.), lebten alle Bakterien in rohem Wasser langer als in gekochtem Wasser. 2) war die Bakterienmenge relativ gross (1 Ose in 10 cc.), lebten die Bakterien zwar langer als bei geringer Menge, verschwanden aber in rohem Wasser schneller als in gekochtem. 3) Unter beiden Versuchsanordnungen lebten die Bakterien im Brunnenwasser langer als in Aq. dest. oder in physiologischer Kochsalzlosung. 4) Die Tabellen zeigen kurz die Ergebnisse: (Autoreferat

Generation of ESTs for Flowering Gene Discovery and SSR Marker Development in Upland Cotton

BACKGROUND: Upland cotton, Gossypium hirsutum L., is one of the world's most important economic crops. In the absence of the entire genomic sequence, a large number of expressed sequence tag (EST) resources of upland cotton have been generated and used in several studies. However, information about the flower development of this species is rare. METHODOLOGY/PRINCIPAL FINDINGS: To clarify the molecular mechanism of flower development in upland cotton, 22,915 high-quality ESTs were generated and assembled into 14,373 unique sequences consisting of 4,563 contigs and 9,810 singletons from a normalized and full-length cDNA library constructed from pooled RNA isolated from shoot apexes, squares, and flowers. Comparative analysis indicated that 5,352 unique sequences had no high-degree matches to the cotton public database. Functional annotation showed that several upland cotton homologs with flowering-related genes were identified in our library. The majority of these genes were specifically expressed in flowering-related tissues. Three GhSEP (G. hirsutum L. SEPALLATA) genes determining floral organ development were cloned, and quantitative real-time PCR (qRT-PCR) revealed that these genes were expressed preferentially in squares or flowers. Furthermore, 670 new putative microsatellites with flanking sequences sufficient for primer design were identified from the 645 unigenes. Twenty-five EST-simple sequence repeats were randomly selected for validation and transferability testing in 17 Gossypium species. Of these, 23 were identified as true-to-type simple sequence repeat loci and were highly transferable among Gossypium species. CONCLUSIONS/SIGNIFICANCE: A high-quality, normalized, full-length cDNA library with a total of 14,373 unique ESTs was generated to provide sequence information for gene discovery and marker development related to upland cotton flower development. These EST resources form a valuable foundation for gene expression profiling analysis, functional analysis of newly discovered genes, genetic linkage, and quantitative trait loci analysis

Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1

A strain of Ralstonia sp. FD-1 capable of using 4-fluoroaniline (4-FA) as the sole carbon and nitrogen source was investigated for its ability to utilize 4-FA isomers (2-FA, 3-FA) and homologs (2,4-DFA, 3,4-DFA, and 2,3,4-TFA). Both 4-FA and 3-FA could be mineralized as the sole carbon and nitrogen source by FD-1. 2-FA, 2,4-DFA, 3,4-DFA, and 2,3,4-TFA could not be degraded by FD-1, respectively, and were selected as secondary substrates for cometabolism with 500 mg/L 4-FA as growth substrate. Bacterial growth (OD600), F− concentrations, and fluoroanilines contents were measured to determinate the degradation ability of 4-FA isomers and homologs by FD-1. FD-1 growth was inhibited by 2,4-DFA, 3,4-DFA, and 2,3,4-TFA at higher concentrations (400 mg/L), except for 2-FA. Complete fluoroanilines degradation was achieved while incomplete defluorination was characterized by the stoichiometric fluoride release indicating partial degradation but not total mineralization. When fluoroaniline was supplied to the resting cells of strain FD-1, a relatively effective removal was showed. Strain FD-1 had broadened application prospect of toxicity and low nutrition fluoroanilines wastewater

The Measurement and Temporal and Spatial Evolution of Tourism Poverty Alleviation Efficiency in the Liupan Mountain Area of Gansu Province, China

Poverty is a chronic problem all over the world. Since the 18th National Congress of the Communist Party of China was held in 2012, the Chinese government has continuously increased its poverty alleviation efforts. In addition, absolute poverty in China was eliminated by the end of 2020. Tourism has played an important role in poverty alleviation. To explore the role of the tourism industry in reducing poverty, this paper takes the Liupan Mountain area of Gansu Province in western China, as a case site. An input–output evaluation index system was constructed, and a Super-SBM model was used to evaluate the tourism poverty alleviation efficiency in 40 districts and counties in the Liupan Mountain area from 2009 to 2018. The results show that the overall starting point of tourism poverty alleviation efficiency in the study area was high, which needed to be further optimized. The difference in tourism poverty alleviation efficiency among districts and counties tended to decrease in the time series. The restrictive factors were the lack of technological progress and lack of innovation. The spatial distribution of tourism poverty alleviation efficiency in the study area presented a pattern of high in the east and low in the west, high in the north and low in the south. In the future, the tourism poverty alleviation efficiency in the concentrated and contiguous destitute areas of the Liupan Mountain area in Gansu Province could be improved by cultivating the tourism industry, strengthening technological innovation, cultivating core growth poles, and exerting radiation effects

The Measurement and Temporal and Spatial Evolution of Tourism Poverty Alleviation Efficiency in the Liupan Mountain Area of Gansu Province, China

Poverty is a chronic problem all over the world. Since the 18th National Congress of the Communist Party of China was held in 2012, the Chinese government has continuously increased its poverty alleviation efforts. In addition, absolute poverty in China was eliminated by the end of 2020. Tourism has played an important role in poverty alleviation. To explore the role of the tourism industry in reducing poverty, this paper takes the Liupan Mountain area of Gansu Province in western China, as a case site. An input–output evaluation index system was constructed, and a Super-SBM model was used to evaluate the tourism poverty alleviation efficiency in 40 districts and counties in the Liupan Mountain area from 2009 to 2018. The results show that the overall starting point of tourism poverty alleviation efficiency in the study area was high, which needed to be further optimized. The difference in tourism poverty alleviation efficiency among districts and counties tended to decrease in the time series. The restrictive factors were the lack of technological progress and lack of innovation. The spatial distribution of tourism poverty alleviation efficiency in the study area presented a pattern of high in the east and low in the west, high in the north and low in the south. In the future, the tourism poverty alleviation efficiency in the concentrated and contiguous destitute areas of the Liupan Mountain area in Gansu Province could be improved by cultivating the tourism industry, strengthening technological innovation, cultivating core growth poles, and exerting radiation effects