Application experience of artificial perfusion combined with interception basket in ureteral calculi lithotripsy

Objective To evaluate clinical efficacy of artificial perfusion combined with interception basket in ureteral calculi lithotripsy. Methods Clinical data of 151 patients with ureteral calculi who underwent surgical treatment were retrospectively analyzed. They were divided into the observation group（artificial perfusion combined with interception basket）and control group（conventional perfusion pump）. The operation time，intraoperative blood loss，proportion of stone escape，stone re-intervention and complications were observed in two groups. Results There were no significant differences in operation time，intraoperative blood loss and incidence of complications between two groups（all P > 0.05）. The proportion of stone escape and stone re-intervention in the observation group were significantly lower than those in the control group，and the differences were statistically significant（both P < 0.05）. Conclusion Artificial low-pressure perfusion combined with interception basket in ureteral stone lithotripsy can reduce the proportion of stone escape and residual stone re-intervention and improve the stone clearance efficiency

A NEW METHOD FOR PREDICTING FATIGUE LIFE UNDER TENSION-TORSIONAL LOADING

The established nonlinear fatigue damage model based on the theory of damage mechanics has been widely applied to multiaxial fatigue life prediction,however,this method does not consider the physical significance in the damage plane.This problem can be solved by combining with the critical plane method.Taking the thin-walled cylindrical specimen as the study object,a new damage parameter applicable to proportional and nonproportional multiaxial fatigue damage is proposed by means of von Mises rule based on the critical plane method with consideration of the influences of phase delay on the fatigue life are analyzed as well.The new defined damage parameter not only considers the maximum shear stain and normal strain on the critical plane,but also considers the effect of the additional cyclic hardening caused by the nonproportional loading on the fatigue life.The fatigue life predicted by the proposed damage model of three types of materials are proved to be in good agreement with experimental results.It is convenient for engineering application because the uniaxial fatigue data and material constants are considered only in this model

Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae

Abstract Microbial proteins are promising substitutes for animal- and plant-based proteins. S. cerevisiae, a generally recognized as safe (GRAS) microorganism, has been frequently employed to generate heterologous proteins. However, constructing a universal yeast chassis for efficient protein production is still a challenge due to the varying properties of different proteins. With progress in synthetic biology, a multitude of molecular biology tools and metabolic engineering strategies have been employed to alleviate these issues. This review first analyses the advantages of protein production by S. cerevisiae. The most recent advances in improving heterologous protein yield are summarized and discussed in terms of protein hyperexpression systems, protein secretion engineering, glycosylation pathway engineering and systems metabolic engineering. Furthermore, the prospects for efficient and sustainable heterologous protein production by S. cerevisiae are also provided

Identifying climatic factors and circulation indices related to apple yield variation in main production areas of China

Assessing the impacts of future climate change on crop yields requires basic research into both the major meteorological factors that affect crop yield and the influence that changes to global circulation patterns have on local meterological parameters. For example, the atmospheric circulation and crop “meteorological yield” and the response of meteorological yield to climate change are positively correlated. Using apple (Malus domestica) production as an example, the present work investigated the link between fruit yield and global circulation factors by using yield data from 28 apple-producing counties in Shaanxi province, China, from 1980 to 2015. We used four methods to isolate apple production and applied a Grey relational analysis of 88 meteorological factors and the associated sequential correlation. We extracted the major climate factors based on the high consistency of climatic factors and the variation trend of meteorological yield of apple, and analyzed the main meteorological factors for the meteorological apple yield of three major apple producing areas by analyzing high-impact atmospheric circulation indices. The climate factors included solar radiation, pan evaporation, accumulated temperature, precipitation, wind speed, maximum temperature, minimum temperature, mean temperature, heat injury over several days, number of frost days during flowering, number of freezing days during dormancy, and annual temperature range. The main meteorological factors affecting annual apple meteorological yield were total solar radiation from April to October, evaporation from April to September, precipitation in April and June to August, and minimum temperature in mid-April. The Southern Oscillation Index (SOI) was the circulation index most closely related to total summer solar radiation, pan evaporation, and precipitation. Correlation analysis showed that the polar vortex area index and sea surface temperature index were important circulation indices affecting climate yield in apple regions. The meteorological yields of apples in zones Ⅰ and Ⅲ were significantly correlated with the ENSO period index, mainly in the autumn and winter. Similar to the conclusions of previous studies, the uncertainty of meteorological yield in the Shaanxi fruit region is predicted to increase under future climate change. Keywords: Apple yield, Shaanxi province, Grey relational analysis, Climate factors, Circulation inde

A summary of genetic effects of <i>QTsg.osu-3A</i> on seed germination under various temperatures.

<p>A summary of genetic effects of <i>QTsg.osu-3A</i> on seed germination under various temperatures.</p

Genetic effect of <i>TaMFT-A1</i> on germination rate.

<p>The germination rate was averaged from each of the Jagger allele (A) or the 2174 allele (B) in the population (n = 96) that were characterized at high temperature (HT) and normal temperature (NT) in 2009 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073330#pone-0073330-g006" target="_blank">Fig. 6A</a>) and 2010 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073330#pone-0073330-g006" target="_blank">Fig. 6B</a>), when seed was harvested 15, 30, 45, or 70 days. Bar indicates standard error.</p

Chromosomal location and genetic effects of <i>QTsg.osu-3A</i> for seed germination.

<p>The QTLs were characterized at high temperature (HT) and normal temperature (NT) in years 2008, 2009, and 2010, when seed was harvested 15, 30, or 45 days. Germination rate was tested in the recombinant inbred lines (RILs) of the Jagger×2174 population. Molecular markers along the chromosome are placed as centimorgans on the horizontal axis. The horizontal dotted line represents a common threshold value of 2.5 LOD.</p

A PCR marker for <i>TaMFT-A1</i>.

<p>Primer MFT-A1F2 and MFT-A1R2 were used to amplify <i>TaMFT-A1</i> from Jagger (331 bp) and 2174 (319 bp). PCR products were directly run on a 1% agarose gel.</p