4 research outputs found
Performance of different illegal transaction contract detection models.
Performance of different illegal transaction contract detection models.</p
Represents the result of clustering by Kmeans after removing the full concatenation layer of the last layer in Tranmulti-View Net, and the best result is displayed in bold.
Represents the result of clustering by Kmeans after removing the full concatenation layer of the last layer in Tranmulti-View Net, and the best result is displayed in bold.</p
Vascularization in Engineered Tissue Construct by Assembly of Cellular Patterned Micromodules and Degradable Microspheres
Tissue engineering aims to generate
functional tissue constructs in which proper extracellular matrix
(ECM) for cell survival and establishment of a vascular network are
necessary. A modular approach via the assembly of modules mimicking
the complex tissues’ microarchitectural features and establishing
a vascular network represents a promising strategy for fabricating
larger and more complex tissue constructs. Herein, as a model for
this modular tissue engineering, engineered bone-like constructs were
developed by self-assembly of osteon-like modules and fast degradable
gelatin microspheres. The collagen microspheres acting as osteon-like
modules were developed by seeding human umbilical vein endothelial
cells (HUVECs) onto collagen microspheres laden with human osteoblast-like
cells (MG63) and collagenase. Both HUVECs and MG63 cells were well
spatially patterned in the modules, and collagen as ECM well supported
cell adhesion, spreading, and functional expression due to its native
RGD domains and enzymatic degradation activity. The patterned modules
facilitated both the cellular function expression of osteogenic MG63
cells and vasculogenic HUVECs; that is, the osteon-like units were
successfully achieved. The assembly of the osteon-like modules and
fast degradable gelatin microspheres promoted the vascularization,
thus facilitating the osteogenic function expression. The study provides
a highly efficient approach to engineering complex 3D tissues with
micropatterned cell types and interconnected channels