TtOmp85, a β‑Barrel Assembly Protein, Functions by Barrel Augmentation

Outer membrane proteins are vital for Gram-negative bacteria and organisms that inherited organelles from them. Proteins from the Omp85/BamA family conduct the insertion of membrane proteins into the outer membrane. We show that an eight-stranded outer membrane β-barrel protein, TtoA, is inserted and folded into liposomes by an Omp85 homologue. Furthermore, we recorded the channel conductance of this Omp85 protein in black lipid membranes, alone and in the presence of peptides comprising the sequence of the two N-terminal and the two C-terminal β-strands of TtoA. Only with the latter could a long-living compound channel that exhibits conductance levels higher than those of the Omp85 protein alone be observed. These data support a model in which unfolded outer membrane protein after docking with its C-terminus penetrates into the transmembrane β-barrel of the Omp85 protein and augments its β-sheet at the first strand. Augmentation with successive β-strands leads to a compound, dilated barrel of both proteins

THE ORGANISATION OF THE INSTRUMENT PREPARATION OF PRODUCTION AT THE EARLY STAGES OF THE ITEM MAKING

The scientific principles of building of the organisation forms of the production preparation have been developed, theoretically justified has been the application of the parallel-consequence form of organisation of the instrument preparation of the production at the early stages of the item producing. The complex of methods, realizing the functions of organisation of the production instrument preparation, using the methods of optimization of the organisation decisions has been developed. This will allow to give the effective evaluation of the variants of equipment and to design its optimum volume. Decision of the problem of the optimum equipment selection, the design of the optimum volumes of the equipment at the early stages of the item make have been accepted for use in the Permsky Unit Design Bureau. The methodical developments on the organisation and economic aspects have been introduced in the study processAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio