Results of DNA-DNA hybridization.

a<p>Because % similarities were shown as the degree of DNA-DNA reassociation calculated based on OD₄₀₅ values obtained as the result of enzyme reaction, they can be greater than 100%.</p>b<p><i>M. plutonius</i>-like isolates.</p>c<p><i>M. plutonius</i> isolates.</p>d<p><i>E. faecalis</i> type strain.</p

Formulas of culture media used in this study.

<p>Unit: g/L.</p><p>Medium 1 to 6 and carbohydrate test media were autoclaved at 115°C for 10 min. Other media were autoclaved at 121°C for 15 min.</p>a<p>The pH was adjusted to 6.6 with KOH.</p>b<p>The pH was adjusted to 6.6 with the solution, in which the mole ratio of KOH/NaOH was 1∶1.</p>c<p>The pH was adjusted to 6.6 with HCl.</p>d<p>The pH was adjusted to 6.6 with NaOH.</p>e<p>Potassium and sodium salts were added to the media to final concentrations of 0.033 M (Medium 4), 0.1 M (Medium 1, 3, 5 and 6) or 0.15 M (KSBHI and KBHI).</p>f<p>D-cellobiose, lactose, D-raffinose, or D-xylose</p>g<p>After the base medium was autoclaved, the carbohydrate was added aseptically.</p

Culture characteristics of <i>M. plutonius</i> and <i>M. plutonius</i>-like strain/isolates used in this study.

a<p>The growth of ATCC 35311 cultured on KSBHI agar under anaerobic conditions was scored as +. Compared to this growth, more vigorous and weaker growth was scored as +^v and +^w, respectively. No growth or only trace levels of growth was scored as −.</p

Dendrogram of SmaI-digested PFGE profiles of typical and atypical <i>M. plutonius</i> strain/isolates.

<p>Phenotypically distinct strain/isolates were also grouped separately into two distinct genetic clusters.</p

Biochemical characteristics of <i>M. plutonius</i> and <i>M. plutonius</i>-like strain/isolates used in this study.

a<p>Repeated tests showed slightly different results in ATCC 35311.</p>b<p>Mannitol, sucrose, maltose, D-xylose, glycerin, D-melezitose, D-raffinose, D-sorbitol, L-rhamnose, and D-trehalose.</p