Molecular mechanisms in idiopathic inflammatory myopathies

Background: Myositis is a group of rare autoimmune diseases. Muscle weakness and fatigue are the dominant symptoms and inflammation with T cells and macrophages is a characteristic finding in muscle tissue. Currently high-dose and long-term glucocorticoids is still the most important treatment but with limited efficacy and worrisome side effects. Therefore, investigations regarding inflammatory mediators and their roles in myositis pathogenesis are important in order to develop new therapies. Methods: In order to investigate the roles of different inflammatory mediators in patients with myositis, biological samples were investigated from clinically well-characterized patients in different phases of disease. Several techniques were employed: immunohistochemical staining on muscle tissue and cultured skeletal muscle cells; flow cytometry, ELISA and chemiluminescence immunoassay on blood samples. Hypotheses regarding molecular mechanisms for muscle weakness and fatigue were tested in animal models, where we mainly used enzymatically dissociated muscle fibers and mechanically dissected muscle fibers to measure the force and calcium release from the sarcoplasmic reticulum under various defined molecular conditions. Results: Based on previous observations on IL-1 expression in muscle tissue we first tested the role of IL-1 by using IL-1 blockade, anakinra, for 12 months in patients with refractory myositis. Eight of 15 patients had a clinical response which correlated to some response in biomarkers in blood, but muscle tissue inflammation persisted. Therefore, we searched for a new immune modulating target and found IL-15 to be expressed in muscle tissue of patients with myositis, and higher IL-15 expression was associated with more muscle dysfunction. Another approach was to test for the role of inflammatory molecules in early phases of muscle inflammation. In this context the extra nuclear presence of the alarmin high-mobility group box 1 (HMGB1) in the muscle fibers of patients with myositis without detectable inflammatory infiltrates is of interest. By in vitro experiments we showed that HMGB1 can influence muscle function by accelerating muscle fatigue and inducing MHC-class I expression via TLR4. Another receptor for HMGB1, TLR2, was also found in muscle tissue of patients with myositis and our animal study demonstrated that by knocking out TLR2 the skeletal muscle fibers became more muscle fatigue resistant. Conclusion: Collectively, the investigations in my thesis suggest that inflammatory mediators play important roles in the pathogenesis of myositis and different molecules may contribute in different phases of disease. Hereby, HMGB1 might induce muscle dysfunction at an early stage of the disease via TLR4 (or/and TLR2). IL-15 could be involved in developing muscle dysfunction via maintaining T cell homeostasis in the muscles. IL-1 may be important in a subset of patients by driving the adaptive immune system. However, more studies are needed for a comprehensive understanding of their roles in order to develop new therapies

Characterization of the Three-Dimensional Fivefold Translative Tiles

This paper proves the following statement: If a convex body can form a fivefold translative tiling in $\mathbb{E}^3$, it must be a parallelotope, a hexagonal prism, a rhombic dodecahedron, an elongated dodecahedron, a truncated octahedron, a cylinder over a particular octagon, or a cylinder over a particular decagon, where the octagon and the decagon are fivefold translative tiles in $\mathbb{E}^2$. Furthermore, it presents an example of multiple tiles in $\mathbb{E}^3$ with multiplicity at most 10 which is neither a parallelohedron nor a cylinder

Optimized Heating Rate and Soot-catalyst Ratio for Soot Oxidation over MoO3 Catalyst

MoO3 is now utilized as a promising catalyst due to its high activity and favorable mobility at low temperature. Its spectral data and surface microstructures were characterized by Fourier transform infrared spectra (FT-IR) and Field emission scanning electron microscope (FESEM). Thermo-analysis of the carbon black was performed over nano-MoO3 catalyst in a thermogravimetric analyzer (TGA) at various heating rates and soot-catalyst ratios. Through the analysis of kinetic parameters, we found that the heat transfer effect and diffusion effect can be removed by setting lower heating rates and soot-catalyst ratios. Therefore, a strategy for selecting proper thermogravimetric parameters were established, which can contribute to the better understanding of thermo-analytical process.

Optimized Heating Rate and Soot-catalyst Ratio for Soot Oxidation over MoO3 Catalyst

MoO3 is now utilized as a promising catalyst due to its high activity and favorable mobility at low temperature. Its spectral data and surface microstructures were characterized by Fourier transform infrared spectra (FT-IR) and Field emission scanning electron microscope (FESEM). Thermo-analysis of the carbon black was performed over nano-MoO3 catalyst in a thermogravimetric analyzer (TGA) at various heating rates and soot-catalyst ratios. Through the analysis of kinetic parameters, we found that the heat transfer effect and diffusion effect can be removed by setting lower heating rates and soot-catalyst ratios. Therefore, a strategy for selecting proper thermogravimetric parameters were established, which can contribute to the better understanding of thermo-analytical process. Copyright © 2017 BCREC Group. All rights reserved Received: 4th December 2016; Revised: 13rd June 2017; Accepted: 9th April 2017; Available online: 27th October 2017; Published regularly: December 2017 How to Cite: Mei, C., Mei, D., Yue, S, Chen, Z., Yuan, Y. (2017). Optimized Heating Rate and Soot-catalyst Ratio for Soot Oxidation over MoO3 Catalyst. Bulletin of Chemical Reaction Engineering & Catalysis, 12 (3): 408-414 (doi:10.9767/bcrec.12.3.845.408-41