Determination of macro and trace element levels of serum, tears, saliva, and hair samples in kilis goats with icp-ms

This study is focused on the evaluation of macro and trace elements concentration including sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), copper (Cu), zinc (Zn), selenium (Se), nickel (Ni), titanium (Ti), manganese (Mn), cobalt (Co), and chromium (Cr) of the serum, saliva, tears, and hair samples in Kilis goats. The study involved 33 goats without clinical signs of disease. Individual serum, saliva, tears, and hair samples from each goat were collected for element quantifications through the inductively coupled plasma mass spectrometry (ICP-MS) method after acid digestion in a microwave system. Element concentrations varied and depended on the sample type. The most predominant element in serum and saliva samples was Na (3559±55.2 and 3265±53.6 mg/L, respectively), followed by K>Ca>Mg. Potassium was the most abundant element analyzed in tears and hair samples (3506±305 and 4664±100.5 mg/L, respectively). The major trace element was Fe in all sample types except hair samples. Nickel was detected only in serum samples (83.9±6.35 μg/L) and Mn was detected only in hair samples (12903±3142 μg/L). In all samples, Co was below the detection limit. There was a significant correlation of some elements between serum and hair (Na, K, Mg, Cu), tears and hair (K, Mg, Fe), serum and tears (K) samples. Trace elements concentration did not pose a risk for deficiency or excess. It was concluded that tears and saliva are potential alternative materials for element analysis and simultaneous tears, saliva, and hair along with serum could be useful in predicting the inorganic metabolic status of goats

Serum biochemical values of Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turkey

Serum biochemical values were determined in clinically healty 12 adult (>12 month) female Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turkey. In the present study, we determined the concentrations of glucose (72.4 ± 24.9 mg/dL), total bilirubin (0.31 ± 0.1 mg/dL), blood urea nitrogen (15.6 ± 4.39 mg/dL), creatinine (0.9 ± 0.07 mg/dL), uric acid (0.7 ± 0.33mg/dL), total protein (7.16 ± 0.33 g/dL), albumin (3.27 ± 0.47 g/dL), cholesterol (52.2 ± 12.72 mg/dL), triglyceride (51.24 ± 17.05 mg/dL) sodium (152.1 ± 4.79 mEq/L), potassium (5.08 ± 0.39 mEq/L), calcium (9.1 ± 0.84 mg/dL), phosphorous (6.6 ± 0.54 mg/dL), chloride (110 ± 3.0 mEq/L), magnesium (1.98 ± 0.08 mg/dL). Furthermore the activity of enzymes AST (189.8 ± 103.7 IU/L), ALT (12.20 ± 9.60 IU/L), ALP (103.8 ± 119.2 IU/L), GGT (50.8 ± 52.5 IU/L), LDH (520.6 ± 179.8 IU/L), CK (194.6 ± 113.8 IU/L), amylase (75.2 ± 32.8 IU/L) were measured. In conclusion we determined reference biochemical values and health status’ of Gazella subgutturosa in Ceylanpınar, Şanlıurfa, TurkeSerum biochemical values were determined in clinically healty 12 adult (>12 month) female Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turkey. In the present study, we determined the concentrations of glucose (72.4 ± 24.9 mg/dL), total bilirubin (0.31 ± 0.1 mg/dL), blood urea nitrogen (15.6 ± 4.39 mg/dL), creatinine (0.9 ± 0.07 mg/dL), uric acid (0.7 ± 0.33mg/dL), total protein (7.16 ± 0.33 g/dL), albumin (3.27 ± 0.47 g/dL), cholesterol (52.2 ± 12.72 mg/dL), triglyceride (51.24 ± 17.05 mg/dL) sodium (152.1 ± 4.79 mEq/L), potassium (5.08 ± 0.39 mEq/L), calcium (9.1 ± 0.84 mg/dL), phosphorous (6.6 ± 0.54 mg/dL), chloride (110 ± 3.0 mEq/L), magnesium (1.98 ± 0.08 mg/dL). Furthermore the activity of enzymes AST (189.8 ± 103.7 IU/L), ALT (12.20 ± 9.60 IU/L), ALP (103.8 ± 119.2 IU/L), GGT (50.8 ± 52.5 IU/L), LDH (520.6 ± 179.8 IU/L), CK (194.6 ± 113.8 IU/L), amylase (75.2 ± 32.8 IU/L) were measured. In conclusion we determined reference biochemical values and health status’ of Gazella subgutturosa in Ceylanpınar, Şanlıurfa, Turk

Some haematological values in Awassi sheep breeding in Şanlıurfa area

Bu araştırma Şanlıurfa yöresinde yetiştirilen Ivesi koyunlarının bazı kan değerlerini belirlemek amacıyla yapıldı. Materyal olarak 1-4 yaşlan arasında, 107 İvesi ırkı koyun kullanıldı. Hayvanlar yaş gruplarına ayrılarak kan alındı. Alınan kan örneklerinde alyuvar ve akyuvar sayısı, hemoglobin miktarı, hematokrit değer, sedimentasyon hızı ve akyuvar tiplerinin yüzde oranları saptandı. Ayrıca, alyuvar sayısı, hemoglobin miktarı ve hematokrit değer kullanılarak ortalama alyuvar hacmi (OAH), ortalama alyuvar hemoglobini (OAHb) ve ortalama alyuvar hemoglobin derişimleri (OAHbD) hesaplandı. Yaş grupları arasındaki istatistiksel farklılık One-Way ANOWA ve Duncan testi ile değerlendirildi. Ivesi koyunları için belirlenen kan değerlerinin, Hamdani, Tuj ve Morkaraman koyunlarının' kan değerlerinden bazılarına benzerlik gösterdiği tespit edildi. Ayrıca, bu ırkın erkeklerinde akyuvar sayısının yaşın ilerlemesi ile birlikte azaldığı gözlemlendi.This study was conducted to examine the some basic haematological parameters of Awassi sheep located in Şanlıurfa region. The investigation was carried out on 107 healthy Awassi sheep aged between 1-4 years old. Blood samples obtained from the animals, separated according to age groups, were analysed for leukocyte (WBC) and erythrocyte (RBC) counts, haemoglobin concentration, haematocrit values (PCV), sedimentation rates and percentages of leukocyte types. Mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were also calculated from the values of RBC, PCV and haemoglobin. Different age groups were assessed by analysis of variance (One-Way ANOWA) and Duncan's multiple range test. These results indicate that blood parameters of Awassi sheep are similar to the some of blood parameters of Hamdani, Tuj and Morkaraman sheep. Beside this, leukocyte numbers decreased in parallel with increased age of male Awassi sheep