Evaluation of anxiolytic potential of Linum usitatissimum oil in wistar rats

The objective of the present study was to evaluate the anxiolytic potential of the Linum usitatissimum (Flax seed) oil (5ml and 2.5ml/kg) in wistar rats by using Elevated plus maze (EPM) model and 5ml and 10 ml/kg in Lithium induced head twitches model. Experiments were carried out on white inbred Wistar rats (180-200 g). The efficacy of the oil at both the animal models was compared with the standard anxiolytic drugs Diazepam (0.5 mg/kg). The result showed that the oil significantly increased the number of entries and time spent in the open arm in the elevated plus maze. Similarly in Lithium induced head twitches model administration of oil decreases the no. of head twitches. Present study confirms that the extract showed significant anxiolytic activity at both dose levels which is comparable with standard anxiolytic Diazepam

Evaluation of Hepatoprotective and In-Vivo Antioxidant Activity of Tamarindus indica L. Seeds Extracts in Streptozotocin Induced Diabetic Rats

Hydroalcoholic and aqueous extract of Tamarindus indica seeds was evaluated for hepatoprotective and antioxidant activity in Streptozotocin induced diabetic rats. Diabetes induced hepatic damage was evaluated by the serum markers such as SGOT, SGPT, ALP, Bilirubin. Further the effect of HAETIS and AETIS on oxidative stress was determined by the markers of oxidative stress: Lipid peroxidation (LPO), Determination of Super Oxide Dismutase (SOD), Determination of Catalase (CAT), glutathione (GSH) were estimated in liver tissue. HAETIS and AETIS at a dose level of  100 mg/kg and 200 mg/kg produce significant (P< 0.05) hepatoprotection by decreasing the activity of serum enzymes, biliribin and lipid perioxidation, while they significantly increase the level of Glutathione, Super Oxide Dismutase and catalase in a dose dependant manner. Histo-pathological studies supports the above finding results indicate that hydroalcoholic extracts of Tamarindus indica at 200 mg/kg showed more significant hepatic protection as compared to other extracts

Evaluation of Hepatoprotective and In-Vivo Antioxidant Activity of Tamarindus indica L. Seeds Extracts in Streptozotocin Induced Diabetic Rats

Hydroalcoholic and aqueous extract of Tamarindus indica seeds was evaluated for hepatoprotective and antioxidant activity in Streptozotocin induced diabetic rats. Diabetes induced hepatic damage was evaluated by the serum markers such as SGOT, SGPT, ALP, Bilirubin. Further the effect of HAETIS and AETIS on oxidative stress was determined by the markers of oxidative stress: Lipid peroxidation (LPO), Determination of Super Oxide Dismutase (SOD), Determination of Catalase (CAT), glutathione (GSH) were estimated in liver tissue. HAETIS and AETIS at a dose level of  100 mg/kg and 200 mg/kg produce significant (P< 0.05) hepatoprotection by decreasing the activity of serum enzymes, biliribin and lipid perioxidation, while they significantly increase the level of Glutathione, Super Oxide Dismutase and catalase in a dose dependant manner. Histo-pathological studies supports the above finding results indicate that hydroalcoholic extracts of Tamarindus indica at 200 mg/kg showed more significant hepatic protection as compared to other extracts

Antioxidant and Antimicrobial Properties of Tamarindus indica L.

In this study, in vitro antioxidant and antimicrobial activities of aqueous and Hydroalcoholic extracts of leaves Tamarindus indica were investigated. In-vitro antioxidant assay was performed by DPPH (1, 1 –diphenyl-2-picrylhydrazyl) assay, Hydroxyl radicals scavenging assay, Ferric reducing assay, Nitric oxide radical scavenging assay. Percentage of free radical scavenging potential was determined using ascorbic acid as a standard in each method. In Ferric reducing antioxidant power assay, antioxidant power was determined by measuring ferric reducing values.  It was observed that hydroalcoholic extracts were having more potential antioxidants. Hydroalcoholic extract at 500 µg/ml shown maximum FRAP value 0.76±0.08.   IC50 values were determined for each extracts. Lowest IC 50 value was exhibited by HAETIS in DPPH, Hydroxyl radical scavenging, Ferric reducing and NO scavenging methods are:   195.30µg/ml,182.02µg/ml,196.23µg/ml respectively. These results indicated that the anti oxidant capacities depends on concentration and nature of extracts. Antimicrobial activities were evaluated by determining inhibition zone diameter and calculated MIC values. Aqueous extracts of Tamarindus indica leaves shown maximum inhibition zone diameter against gram positive bacteria Staphylococcus aureus 17.33±1.5 mm and was exhibited by Ciprofloxacin used as standard 36.0±2.0mm. In gram negative bacteria Pseudomonas aeruginosa hydroalcoholic extracts of Tamarindus indica leaves shown 24.66±.51mm and Gentamicin used as standard 37.0±1.73. Aqueous extracts of leaves shown good antimicrobial activity against gram positive bacteria and hydroalcoholic extracts of leaves in gram negative bacteria. Minimum inhibitory concentration shown by different extracts were in the range of 8-20mg/ml. Hydroalcoholic extracts of leaves shown  lowest minimum Inhibitory concentration 8mg/ml against Pseudomonas aeruginosa